Highly parallel and efficient single cell mRNA sequencing with paired picoliter chambers

Highly parallel and efficient single cell mRNA sequencing with paired picoliter chambers

Single-cell RNA-seq can reveal accurate and precise cell types and states. Here the authors present an scRNA-seq platform, Paired-seq, which uses differential flow resistance to achieve 95% cell utilisation efficiency for improved cell-free RNA removal and gene detection.

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Bibliographic Details
Main Authors: Mingxia Zhang, Yuan Zou, Xing Xu, Xuebing Zhang, Mingxuan Gao, Jia Song, Peifeng Huang, Qin Chen, Zhi Zhu, Wei Lin, Richard N. Zare, Chaoyong Yang
Format: article
Language:EN
Published: Nature Portfolio 2020
Subjects:
Science
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Online Access:https://doaj.org/article/2803c5a6e28e40b48cbe7529ec370858
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Summary:Single-cell RNA-seq can reveal accurate and precise cell types and states. Here the authors present an scRNA-seq platform, Paired-seq, which uses differential flow resistance to achieve 95% cell utilisation efficiency for improved cell-free RNA removal and gene detection.

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