Highly parallel and efficient single cell mRNA sequencing with paired picoliter chambers

Single-cell RNA-seq can reveal accurate and precise cell types and states. Here the authors present an scRNA-seq platform, Paired-seq, which uses differential flow resistance to achieve 95% cell utilisation efficiency for improved cell-free RNA removal and gene detection.

Guardado en:

Detalles Bibliográficos
Autores principales:	Mingxia Zhang, Yuan Zou, Xing Xu, Xuebing Zhang, Mingxuan Gao, Jia Song, Peifeng Huang, Qin Chen, Zhi Zhu, Wei Lin, Richard N. Zare, Chaoyong Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/2803c5a6e28e40b48cbe7529ec370858
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2803c5a6e28e40b48cbe7529ec370858
record_format	dspace
spelling	oai:doaj.org-article:2803c5a6e28e40b48cbe7529ec3708582021-12-02T17:31:07ZHighly parallel and efficient single cell mRNA sequencing with paired picoliter chambers10.1038/s41467-020-15765-02041-1723https://doaj.org/article/2803c5a6e28e40b48cbe7529ec3708582020-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15765-0https://doaj.org/toc/2041-1723Single-cell RNA-seq can reveal accurate and precise cell types and states. Here the authors present an scRNA-seq platform, Paired-seq, which uses differential flow resistance to achieve 95% cell utilisation efficiency for improved cell-free RNA removal and gene detection.Mingxia ZhangYuan ZouXing XuXuebing ZhangMingxuan GaoJia SongPeifeng HuangQin ChenZhi ZhuWei LinRichard N. ZareChaoyong YangNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Mingxia Zhang Yuan Zou Xing Xu Xuebing Zhang Mingxuan Gao Jia Song Peifeng Huang Qin Chen Zhi Zhu Wei Lin Richard N. Zare Chaoyong Yang Highly parallel and efficient single cell mRNA sequencing with paired picoliter chambers
description	Single-cell RNA-seq can reveal accurate and precise cell types and states. Here the authors present an scRNA-seq platform, Paired-seq, which uses differential flow resistance to achieve 95% cell utilisation efficiency for improved cell-free RNA removal and gene detection.
format	article
author	Mingxia Zhang Yuan Zou Xing Xu Xuebing Zhang Mingxuan Gao Jia Song Peifeng Huang Qin Chen Zhi Zhu Wei Lin Richard N. Zare Chaoyong Yang
author_facet	Mingxia Zhang Yuan Zou Xing Xu Xuebing Zhang Mingxuan Gao Jia Song Peifeng Huang Qin Chen Zhi Zhu Wei Lin Richard N. Zare Chaoyong Yang
author_sort	Mingxia Zhang
title	Highly parallel and efficient single cell mRNA sequencing with paired picoliter chambers
title_short	Highly parallel and efficient single cell mRNA sequencing with paired picoliter chambers
title_full	Highly parallel and efficient single cell mRNA sequencing with paired picoliter chambers
title_fullStr	Highly parallel and efficient single cell mRNA sequencing with paired picoliter chambers
title_full_unstemmed	Highly parallel and efficient single cell mRNA sequencing with paired picoliter chambers
title_sort	highly parallel and efficient single cell mrna sequencing with paired picoliter chambers
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/2803c5a6e28e40b48cbe7529ec370858
work_keys_str_mv	AT mingxiazhang highlyparallelandefficientsinglecellmrnasequencingwithpairedpicoliterchambers AT yuanzou highlyparallelandefficientsinglecellmrnasequencingwithpairedpicoliterchambers AT xingxu highlyparallelandefficientsinglecellmrnasequencingwithpairedpicoliterchambers AT xuebingzhang highlyparallelandefficientsinglecellmrnasequencingwithpairedpicoliterchambers AT mingxuangao highlyparallelandefficientsinglecellmrnasequencingwithpairedpicoliterchambers AT jiasong highlyparallelandefficientsinglecellmrnasequencingwithpairedpicoliterchambers AT peifenghuang highlyparallelandefficientsinglecellmrnasequencingwithpairedpicoliterchambers AT qinchen highlyparallelandefficientsinglecellmrnasequencingwithpairedpicoliterchambers AT zhizhu highlyparallelandefficientsinglecellmrnasequencingwithpairedpicoliterchambers AT weilin highlyparallelandefficientsinglecellmrnasequencingwithpairedpicoliterchambers AT richardnzare highlyparallelandefficientsinglecellmrnasequencingwithpairedpicoliterchambers AT chaoyongyang highlyparallelandefficientsinglecellmrnasequencingwithpairedpicoliterchambers
_version_	1718380661570535424

Highly parallel and efficient single cell mRNA sequencing with paired picoliter chambers

Ejemplares similares