Sublethal pesticide exposure induces larval removal behavior in honeybees through chemical cues

We were intrigued by reported observations of reduced brood production and a high number of empty brood cells in bee colonies exposed to sublethal pesticide doses, which could suggest an active removal of larvae. Higher numbers of oenocytes, insect cells responsible for lipid processing and detoxifi...

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Autores principales:	Wolfgang Schuehly, Ulrike Riessberger-Gallé, Javier Hernández López
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Chemical communication Cuticular hydrocarbons Honeybee larvae Hygienic behavior Sublethal pesticide doses Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/280c45cf0ff145738852b83a769d5c23
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spelling	oai:doaj.org-article:280c45cf0ff145738852b83a769d5c232021-12-02T04:58:55ZSublethal pesticide exposure induces larval removal behavior in honeybees through chemical cues0147-651310.1016/j.ecoenv.2021.113020https://doaj.org/article/280c45cf0ff145738852b83a769d5c232021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321011325https://doaj.org/toc/0147-6513We were intrigued by reported observations of reduced brood production and a high number of empty brood cells in bee colonies exposed to sublethal pesticide doses, which could suggest an active removal of larvae. Higher numbers of oenocytes, insect cells responsible for lipid processing and detoxification, were also found in pesticide-exposed larvae. Oenocytes are involved in hydrocarbon metabolism and chemical communication, and we hypothesized that these larvae could display altered cuticular hydrocarbon (CHC) profiles when exposed to pesticides as compared to control larvae. In addition, we proposed that these chemical cues could trigger specific behavioral responses in colony nurses. To test these hypotheses, we analyzed the CHC profiles of artificially reared larvae that had been fed sublethal doses of either dimethoate or clothianidin or fed on lipopolysaccharide (LPS) using gas chromatography-mass spectrometry. We found significant differences in the CHC profiles of these differently treated larvae. In a subsequent behavioral experiment, we transferred clothianidin-treated or LPS-treated larvae into the brood combs of surrogate colonies. Larvae that had been fed either the pesticide or LPS were removed at a significantly higher rate than control larvae. Our results demonstrate that larvae exposed to clothianidin possess altered CHC profiles, are detected in the colony by nurse bees via chemical cues and are actively removed.Wolfgang SchuehlyUlrike Riessberger-GalléJavier Hernández LópezElsevierarticleChemical communicationCuticular hydrocarbonsHoneybee larvaeHygienic behaviorSublethal pesticide dosesEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 228, Iss , Pp 113020- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Chemical communication Cuticular hydrocarbons Honeybee larvae Hygienic behavior Sublethal pesticide doses Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Chemical communication Cuticular hydrocarbons Honeybee larvae Hygienic behavior Sublethal pesticide doses Environmental pollution TD172-193.5 Environmental sciences GE1-350 Wolfgang Schuehly Ulrike Riessberger-Gallé Javier Hernández López Sublethal pesticide exposure induces larval removal behavior in honeybees through chemical cues
description	We were intrigued by reported observations of reduced brood production and a high number of empty brood cells in bee colonies exposed to sublethal pesticide doses, which could suggest an active removal of larvae. Higher numbers of oenocytes, insect cells responsible for lipid processing and detoxification, were also found in pesticide-exposed larvae. Oenocytes are involved in hydrocarbon metabolism and chemical communication, and we hypothesized that these larvae could display altered cuticular hydrocarbon (CHC) profiles when exposed to pesticides as compared to control larvae. In addition, we proposed that these chemical cues could trigger specific behavioral responses in colony nurses. To test these hypotheses, we analyzed the CHC profiles of artificially reared larvae that had been fed sublethal doses of either dimethoate or clothianidin or fed on lipopolysaccharide (LPS) using gas chromatography-mass spectrometry. We found significant differences in the CHC profiles of these differently treated larvae. In a subsequent behavioral experiment, we transferred clothianidin-treated or LPS-treated larvae into the brood combs of surrogate colonies. Larvae that had been fed either the pesticide or LPS were removed at a significantly higher rate than control larvae. Our results demonstrate that larvae exposed to clothianidin possess altered CHC profiles, are detected in the colony by nurse bees via chemical cues and are actively removed.
format	article
author	Wolfgang Schuehly Ulrike Riessberger-Gallé Javier Hernández López
author_facet	Wolfgang Schuehly Ulrike Riessberger-Gallé Javier Hernández López
author_sort	Wolfgang Schuehly
title	Sublethal pesticide exposure induces larval removal behavior in honeybees through chemical cues
title_short	Sublethal pesticide exposure induces larval removal behavior in honeybees through chemical cues
title_full	Sublethal pesticide exposure induces larval removal behavior in honeybees through chemical cues
title_fullStr	Sublethal pesticide exposure induces larval removal behavior in honeybees through chemical cues
title_full_unstemmed	Sublethal pesticide exposure induces larval removal behavior in honeybees through chemical cues
title_sort	sublethal pesticide exposure induces larval removal behavior in honeybees through chemical cues
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/280c45cf0ff145738852b83a769d5c23
work_keys_str_mv	AT wolfgangschuehly sublethalpesticideexposureinduceslarvalremovalbehaviorinhoneybeesthroughchemicalcues AT ulrikeriessbergergalle sublethalpesticideexposureinduceslarvalremovalbehaviorinhoneybeesthroughchemicalcues AT javierhernandezlopez sublethalpesticideexposureinduceslarvalremovalbehaviorinhoneybeesthroughchemicalcues
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Sublethal pesticide exposure induces larval removal behavior in honeybees through chemical cues

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