Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants

Abstract Replacement orthopedic surgeries are among the most common surgeries worldwide, but clinically used passive implants cannot prevent failure rates and inherent revision arthroplasties. Optimized non-instrumented implants, resorting to preclinically tested bioactive coatings, improve initial...

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Autores principales:	Bárbara M. de Sousa, Clara R. Correia, Jorge A. F. Ferreira, João F. Mano, Edward P. Furlani, Marco P. Soares dos Santos, Sandra I. Vieira
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/281173fd7b014b119611c44e55c55dd1
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spelling	oai:doaj.org-article:281173fd7b014b119611c44e55c55dd12021-11-28T12:38:30ZCapacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants10.1038/s41536-021-00184-62057-3995https://doaj.org/article/281173fd7b014b119611c44e55c55dd12021-11-01T00:00:00Zhttps://doi.org/10.1038/s41536-021-00184-6https://doaj.org/toc/2057-3995Abstract Replacement orthopedic surgeries are among the most common surgeries worldwide, but clinically used passive implants cannot prevent failure rates and inherent revision arthroplasties. Optimized non-instrumented implants, resorting to preclinically tested bioactive coatings, improve initial osseointegration but lack long-term personalized actuation on the bone–implant interface. Novel bioelectronic devices comprising biophysical stimulators and sensing systems are thus emerging, aiming for long-term control of peri-implant bone growth through biointerface monitoring. These acting-sensing dual systems require high frequency (HF) operations able to stimulate osteoinduction/osteoconduction, including matrix maturation and mineralization. A sensing-compatible capacitive stimulator of thin interdigitated electrodes and delivering an electrical 60 kHz HF stimulation, 30 min/day, is here shown to promote osteoconduction in pre-osteoblasts and osteoinduction in human adipose-derived mesenchymal stem cells (hASCs). HF stimulation through this capacitive interdigitated system had significant effects on osteoblasts’ collagen-I synthesis, matrix, and mineral deposition. A proteomic analysis of microvesicles released from electrically-stimulated osteoblasts revealed regulation of osteodifferentiation and mineralization-related proteins (e.g. Tgfb3, Ttyh3, Itih1, Aldh1a1). Proteomics data are available via ProteomeXchange with the identifier PXD028551. Further, under HF stimulation, hASCs exhibited higher osteogenic commitment and enhanced hydroxyapatite deposition. These promising osteoinductive/conductive capacitive stimulators will integrate novel bioelectronic implants able to monitor the bone–implant interface and deliver personalized stimulation to peri-implant tissues.Bárbara M. de SousaClara R. CorreiaJorge A. F. FerreiraJoão F. ManoEdward P. FurlaniMarco P. Soares dos SantosSandra I. VieiraNature PortfolioarticleMedicineRENnpj Regenerative Medicine, Vol 6, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Bárbara M. de Sousa Clara R. Correia Jorge A. F. Ferreira João F. Mano Edward P. Furlani Marco P. Soares dos Santos Sandra I. Vieira Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants
description	Abstract Replacement orthopedic surgeries are among the most common surgeries worldwide, but clinically used passive implants cannot prevent failure rates and inherent revision arthroplasties. Optimized non-instrumented implants, resorting to preclinically tested bioactive coatings, improve initial osseointegration but lack long-term personalized actuation on the bone–implant interface. Novel bioelectronic devices comprising biophysical stimulators and sensing systems are thus emerging, aiming for long-term control of peri-implant bone growth through biointerface monitoring. These acting-sensing dual systems require high frequency (HF) operations able to stimulate osteoinduction/osteoconduction, including matrix maturation and mineralization. A sensing-compatible capacitive stimulator of thin interdigitated electrodes and delivering an electrical 60 kHz HF stimulation, 30 min/day, is here shown to promote osteoconduction in pre-osteoblasts and osteoinduction in human adipose-derived mesenchymal stem cells (hASCs). HF stimulation through this capacitive interdigitated system had significant effects on osteoblasts’ collagen-I synthesis, matrix, and mineral deposition. A proteomic analysis of microvesicles released from electrically-stimulated osteoblasts revealed regulation of osteodifferentiation and mineralization-related proteins (e.g. Tgfb3, Ttyh3, Itih1, Aldh1a1). Proteomics data are available via ProteomeXchange with the identifier PXD028551. Further, under HF stimulation, hASCs exhibited higher osteogenic commitment and enhanced hydroxyapatite deposition. These promising osteoinductive/conductive capacitive stimulators will integrate novel bioelectronic implants able to monitor the bone–implant interface and deliver personalized stimulation to peri-implant tissues.
format	article
author	Bárbara M. de Sousa Clara R. Correia Jorge A. F. Ferreira João F. Mano Edward P. Furlani Marco P. Soares dos Santos Sandra I. Vieira
author_facet	Bárbara M. de Sousa Clara R. Correia Jorge A. F. Ferreira João F. Mano Edward P. Furlani Marco P. Soares dos Santos Sandra I. Vieira
author_sort	Bárbara M. de Sousa
title	Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants
title_short	Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants
title_full	Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants
title_fullStr	Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants
title_full_unstemmed	Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants
title_sort	capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/281173fd7b014b119611c44e55c55dd1
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Capacitive interdigitated system of high osteoinductive/conductive performance for personalized acting-sensing implants

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