Facile green biosynthesis of silver nanoparticles using Pisum sativum L. outer peel aqueous extract and its antidiabetic, cytotoxicity, antioxidant, and antibacterial activity

Jayanta Kumar Patra,1 Gitishree Das,1 Han-Seung Shin21Research Institute of Biotechnology and Medical Converged Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea; 2Department of Food Science & Biotechnology, Dongguk University-Seoul, Goyang 10326, Republic of KoreaCorrespond...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Patra JK, Das G, Shin HS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Antibacterial antidiabetic antioxidant cytotoxicity garden pea silver nanoparticles Pisum sativum Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/28190a2a204144f9ae0a4a3707ef8cb7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:28190a2a204144f9ae0a4a3707ef8cb7
record_format	dspace
spelling	oai:doaj.org-article:28190a2a204144f9ae0a4a3707ef8cb72021-12-02T09:08:48ZFacile green biosynthesis of silver nanoparticles using Pisum sativum L. outer peel aqueous extract and its antidiabetic, cytotoxicity, antioxidant, and antibacterial activity1178-2013https://doaj.org/article/28190a2a204144f9ae0a4a3707ef8cb72019-08-01T00:00:00Zhttps://www.dovepress.com/facile-green-biosynthesis-of-silver-nanoparticles-using-pisum-sativum--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jayanta Kumar Patra,1 Gitishree Das,1 Han-Seung Shin21Research Institute of Biotechnology and Medical Converged Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea; 2Department of Food Science & Biotechnology, Dongguk University-Seoul, Goyang 10326, Republic of KoreaCorrespondence: Han-Seung ShinDepartment of Food Science & Biotechnology, Dongguk University-Seoul, Goyangsi 10326, Republic of KoreaTel +82 31 961 5184Email spartan@dongguk.eduBackground: The synthesis of silver nanoparticles (AgNPs) using food waste materials and their biomedical applications have garnered considerable attention recently.Methods: Here, we investigated the synthesis of AgNPs using an aqueous extract of outer peel of Pisum sativum under different lighting conditions using standard procedures and explored their antidiabetic, cytotoxicity, antioxidant, and antibacterial potential.Results: Characterization of AgNPs was done by Ultra Violet (UV-VIS) spectroscopy that showed absorption maxima at 456 nm for the samples exposed to laboratory lighting and at 464 nm for the samples exposed to direct sunlight, by scanning electron microscopy and energy-dispersive X-ray analysis that showed the surface nature and their elemental composition with a strong peak at 3 keV that corresponded to Ag (61.85 wt%), by Fourier-transform infrared spectroscopy that predicted the functional groups involved, and by X-ray powder diffraction that showed the structural properties. The average diameter of the synthesized AgNPs was calculated to be in the range of 10–25 nm. AgNPs exhibited promising antidiabetic activity as determined by inhibition of α-glucosidase (95.29% inhibition at 10 μg/mL and IC50 value of 2.10 μg/mL) and cytotoxicity (IC50 value 4.0 μg/mL as calculated from the slope equation) against HepG2 cells. Furthermore, they also exhibited moderate antioxidant activity (50.17% reduction of 1,1-diphenyl-2-picrylhydrazyl at 100 μg/mL) and antibacterial activity against four human pathogenic bacteria (as indicated by 8.70–11.10 mm inhibition zones on agar plates).Conclusion: In conclusion, the results confirm that food waste can be used in the synthesis of AgNPs and that the latter have the potential for applications in various fields including diabetic and cancer treatments as well as in biomedicine for the manufacture of antibacterial coatings in medical devices and instruments.Keywords: antibacterial, antidiabetic, antioxidant, cytotoxicity, garden pea, silver nanoparticles, Pisum sativumPatra JKDas GShin HSDove Medical PressarticleAntibacterialantidiabeticantioxidantcytotoxicitygarden peasilver nanoparticlesPisum sativumMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 6679-6690 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Antibacterial antidiabetic antioxidant cytotoxicity garden pea silver nanoparticles Pisum sativum Medicine (General) R5-920
spellingShingle	Antibacterial antidiabetic antioxidant cytotoxicity garden pea silver nanoparticles Pisum sativum Medicine (General) R5-920 Patra JK Das G Shin HS Facile green biosynthesis of silver nanoparticles using Pisum sativum L. outer peel aqueous extract and its antidiabetic, cytotoxicity, antioxidant, and antibacterial activity
description	Jayanta Kumar Patra,1 Gitishree Das,1 Han-Seung Shin21Research Institute of Biotechnology and Medical Converged Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea; 2Department of Food Science & Biotechnology, Dongguk University-Seoul, Goyang 10326, Republic of KoreaCorrespondence: Han-Seung ShinDepartment of Food Science & Biotechnology, Dongguk University-Seoul, Goyangsi 10326, Republic of KoreaTel +82 31 961 5184Email spartan@dongguk.eduBackground: The synthesis of silver nanoparticles (AgNPs) using food waste materials and their biomedical applications have garnered considerable attention recently.Methods: Here, we investigated the synthesis of AgNPs using an aqueous extract of outer peel of Pisum sativum under different lighting conditions using standard procedures and explored their antidiabetic, cytotoxicity, antioxidant, and antibacterial potential.Results: Characterization of AgNPs was done by Ultra Violet (UV-VIS) spectroscopy that showed absorption maxima at 456 nm for the samples exposed to laboratory lighting and at 464 nm for the samples exposed to direct sunlight, by scanning electron microscopy and energy-dispersive X-ray analysis that showed the surface nature and their elemental composition with a strong peak at 3 keV that corresponded to Ag (61.85 wt%), by Fourier-transform infrared spectroscopy that predicted the functional groups involved, and by X-ray powder diffraction that showed the structural properties. The average diameter of the synthesized AgNPs was calculated to be in the range of 10–25 nm. AgNPs exhibited promising antidiabetic activity as determined by inhibition of α-glucosidase (95.29% inhibition at 10 μg/mL and IC50 value of 2.10 μg/mL) and cytotoxicity (IC50 value 4.0 μg/mL as calculated from the slope equation) against HepG2 cells. Furthermore, they also exhibited moderate antioxidant activity (50.17% reduction of 1,1-diphenyl-2-picrylhydrazyl at 100 μg/mL) and antibacterial activity against four human pathogenic bacteria (as indicated by 8.70–11.10 mm inhibition zones on agar plates).Conclusion: In conclusion, the results confirm that food waste can be used in the synthesis of AgNPs and that the latter have the potential for applications in various fields including diabetic and cancer treatments as well as in biomedicine for the manufacture of antibacterial coatings in medical devices and instruments.Keywords: antibacterial, antidiabetic, antioxidant, cytotoxicity, garden pea, silver nanoparticles, Pisum sativum
format	article
author	Patra JK Das G Shin HS
author_facet	Patra JK Das G Shin HS
author_sort	Patra JK
title	Facile green biosynthesis of silver nanoparticles using Pisum sativum L. outer peel aqueous extract and its antidiabetic, cytotoxicity, antioxidant, and antibacterial activity
title_short	Facile green biosynthesis of silver nanoparticles using Pisum sativum L. outer peel aqueous extract and its antidiabetic, cytotoxicity, antioxidant, and antibacterial activity
title_full	Facile green biosynthesis of silver nanoparticles using Pisum sativum L. outer peel aqueous extract and its antidiabetic, cytotoxicity, antioxidant, and antibacterial activity
title_fullStr	Facile green biosynthesis of silver nanoparticles using Pisum sativum L. outer peel aqueous extract and its antidiabetic, cytotoxicity, antioxidant, and antibacterial activity
title_full_unstemmed	Facile green biosynthesis of silver nanoparticles using Pisum sativum L. outer peel aqueous extract and its antidiabetic, cytotoxicity, antioxidant, and antibacterial activity
title_sort	facile green biosynthesis of silver nanoparticles using pisum sativum l. outer peel aqueous extract and its antidiabetic, cytotoxicity, antioxidant, and antibacterial activity
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/28190a2a204144f9ae0a4a3707ef8cb7
work_keys_str_mv	AT patrajk facilegreenbiosynthesisofsilvernanoparticlesusingpisumsativumlouterpeelaqueousextractanditsantidiabeticcytotoxicityantioxidantandantibacterialactivity AT dasg facilegreenbiosynthesisofsilvernanoparticlesusingpisumsativumlouterpeelaqueousextractanditsantidiabeticcytotoxicityantioxidantandantibacterialactivity AT shinhs facilegreenbiosynthesisofsilvernanoparticlesusingpisumsativumlouterpeelaqueousextractanditsantidiabeticcytotoxicityantioxidantandantibacterialactivity
_version_	1718398233999310848

Facile green biosynthesis of silver nanoparticles using Pisum sativum L. outer peel aqueous extract and its antidiabetic, cytotoxicity, antioxidant, and antibacterial activity

Ejemplares similares