Chemoenzymatic Synthesis and Antibody Binding of HIV-1 V1/V2 Glycopeptide-Bacteriophage Q<i>β</i> Conjugates as a Vaccine Candidate

The broadly neutralizing antibody PG9 recognizes a unique glycopeptide epitope in the V1V2 domain of HIV-1 gp120 envelope glycoprotein. The present study describes the design, synthesis, and antibody-binding analysis of HIV-1 V1V2 glycopeptide-Q<i>β</i> conjugates as a mimic of the propo...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Guanghui Zong, Christian Toonstra, Qiang Yang, Roushu Zhang, Lai-Xi Wang
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
Acceso en línea:https://doaj.org/article/282b58a8d3e447fe8c113391fbbbb5e3
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:282b58a8d3e447fe8c113391fbbbb5e3
record_format dspace
spelling oai:doaj.org-article:282b58a8d3e447fe8c113391fbbbb5e32021-11-25T17:57:37ZChemoenzymatic Synthesis and Antibody Binding of HIV-1 V1/V2 Glycopeptide-Bacteriophage Q<i>β</i> Conjugates as a Vaccine Candidate10.3390/ijms2222125381422-00671661-6596https://doaj.org/article/282b58a8d3e447fe8c113391fbbbb5e32021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12538https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The broadly neutralizing antibody PG9 recognizes a unique glycopeptide epitope in the V1V2 domain of HIV-1 gp120 envelope glycoprotein. The present study describes the design, synthesis, and antibody-binding analysis of HIV-1 V1V2 glycopeptide-Q<i>β</i> conjugates as a mimic of the proposed neutralizing epitope of PG9. The glycopeptides were synthesized using a highly efficient chemoenzymatic method. The alkyne-tagged glycopeptides were then conjugated to the recombinant bacteriophage (Q<i>β</i>), a virus-like nanoparticle, through a click reaction. Antibody-binding analysis indicated that the synthetic glycoconjugates showed significantly enhanced affinity for antibody PG9 compared with the monomeric glycopeptides. It was also shown that the affinity of the Q<i>β</i>-conjugates for antibody PG9 was dependent on the density of the glycopeptide antigen display. The glycopeptide-Q<i>β</i> conjugates synthesized represent a promising candidate of HIV-1 vaccine.Guanghui ZongChristian ToonstraQiang YangRoushu ZhangLai-Xi WangMDPI AGarticleglycopeptideglycoconjugateneutralizing epitopebacteriophage Q<i>β</i>neutralizing antibodyHIV vaccineBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12538, p 12538 (2021)
institution DOAJ
collection DOAJ
language EN
topic glycopeptide
glycoconjugate
neutralizing epitope
bacteriophage Q<i>β</i>
neutralizing antibody
HIV vaccine
Biology (General)
QH301-705.5
Chemistry
QD1-999
spellingShingle glycopeptide
glycoconjugate
neutralizing epitope
bacteriophage Q<i>β</i>
neutralizing antibody
HIV vaccine
Biology (General)
QH301-705.5
Chemistry
QD1-999
Guanghui Zong
Christian Toonstra
Qiang Yang
Roushu Zhang
Lai-Xi Wang
Chemoenzymatic Synthesis and Antibody Binding of HIV-1 V1/V2 Glycopeptide-Bacteriophage Q<i>β</i> Conjugates as a Vaccine Candidate
description The broadly neutralizing antibody PG9 recognizes a unique glycopeptide epitope in the V1V2 domain of HIV-1 gp120 envelope glycoprotein. The present study describes the design, synthesis, and antibody-binding analysis of HIV-1 V1V2 glycopeptide-Q<i>β</i> conjugates as a mimic of the proposed neutralizing epitope of PG9. The glycopeptides were synthesized using a highly efficient chemoenzymatic method. The alkyne-tagged glycopeptides were then conjugated to the recombinant bacteriophage (Q<i>β</i>), a virus-like nanoparticle, through a click reaction. Antibody-binding analysis indicated that the synthetic glycoconjugates showed significantly enhanced affinity for antibody PG9 compared with the monomeric glycopeptides. It was also shown that the affinity of the Q<i>β</i>-conjugates for antibody PG9 was dependent on the density of the glycopeptide antigen display. The glycopeptide-Q<i>β</i> conjugates synthesized represent a promising candidate of HIV-1 vaccine.
format article
author Guanghui Zong
Christian Toonstra
Qiang Yang
Roushu Zhang
Lai-Xi Wang
author_facet Guanghui Zong
Christian Toonstra
Qiang Yang
Roushu Zhang
Lai-Xi Wang
author_sort Guanghui Zong
title Chemoenzymatic Synthesis and Antibody Binding of HIV-1 V1/V2 Glycopeptide-Bacteriophage Q<i>β</i> Conjugates as a Vaccine Candidate
title_short Chemoenzymatic Synthesis and Antibody Binding of HIV-1 V1/V2 Glycopeptide-Bacteriophage Q<i>β</i> Conjugates as a Vaccine Candidate
title_full Chemoenzymatic Synthesis and Antibody Binding of HIV-1 V1/V2 Glycopeptide-Bacteriophage Q<i>β</i> Conjugates as a Vaccine Candidate
title_fullStr Chemoenzymatic Synthesis and Antibody Binding of HIV-1 V1/V2 Glycopeptide-Bacteriophage Q<i>β</i> Conjugates as a Vaccine Candidate
title_full_unstemmed Chemoenzymatic Synthesis and Antibody Binding of HIV-1 V1/V2 Glycopeptide-Bacteriophage Q<i>β</i> Conjugates as a Vaccine Candidate
title_sort chemoenzymatic synthesis and antibody binding of hiv-1 v1/v2 glycopeptide-bacteriophage q<i>β</i> conjugates as a vaccine candidate
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/282b58a8d3e447fe8c113391fbbbb5e3
work_keys_str_mv AT guanghuizong chemoenzymaticsynthesisandantibodybindingofhiv1v1v2glycopeptidebacteriophageqibiconjugatesasavaccinecandidate
AT christiantoonstra chemoenzymaticsynthesisandantibodybindingofhiv1v1v2glycopeptidebacteriophageqibiconjugatesasavaccinecandidate
AT qiangyang chemoenzymaticsynthesisandantibodybindingofhiv1v1v2glycopeptidebacteriophageqibiconjugatesasavaccinecandidate
AT roushuzhang chemoenzymaticsynthesisandantibodybindingofhiv1v1v2glycopeptidebacteriophageqibiconjugatesasavaccinecandidate
AT laixiwang chemoenzymaticsynthesisandantibodybindingofhiv1v1v2glycopeptidebacteriophageqibiconjugatesasavaccinecandidate
_version_ 1718411790961868800