Detection of red blood cell surface antigens by probe-triggered cell collision and flow retardation in an autonomous microfluidic system

Abstract Microfluidic devices exploit combined physical, chemical and biological phenomena that could be unique in the sub-millimeter dimensions. The current goal of development of Point-of-Care (POC) medical devices is to extract the biomedical information from the blood. We examined the characteri...

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Autores principales: Éva Sautner, Krisztián Papp, Eszter Holczer, Eszter L. Tóth, Rita Ungai-Salánki, Bálint Szabó, Péter Fürjes, József Prechl
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/2830640cbe9a4312afb7894c52235bdc
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spelling oai:doaj.org-article:2830640cbe9a4312afb7894c52235bdc2021-12-02T15:05:09ZDetection of red blood cell surface antigens by probe-triggered cell collision and flow retardation in an autonomous microfluidic system10.1038/s41598-017-01166-92045-2322https://doaj.org/article/2830640cbe9a4312afb7894c52235bdc2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01166-9https://doaj.org/toc/2045-2322Abstract Microfluidic devices exploit combined physical, chemical and biological phenomena that could be unique in the sub-millimeter dimensions. The current goal of development of Point-of-Care (POC) medical devices is to extract the biomedical information from the blood. We examined the characteristics of blood flow in autonomous microfluidic devices with the aim to realize sensitive detection of interactions between particulate elements of the blood and the appropriately modified surfaces of the system. As a model experiment we demonstrated the fast analysis of the AB0 blood group system. We observed that the accumulation of red blood cells immobilized on the capillary wall leads to increased lateral movement of the flowing cells, resulting in the overall selective deceleration of the red blood cell flow column compared to the plasma fraction. We showed that by monitoring the flow rate characteristics in capillaries coated with blood type reagents it is possible to identify red blood cell types. Analysis of hydrodynamic effects governing blood flow by Finite Element Method based modelling supported our observations. Our proof-of-concept results point to a novel direction in blood analysis in autonomous microfluidic systems and also provide the basis for the construction of a simple quantitative device for blood group determination.Éva SautnerKrisztián PappEszter HolczerEszter L. TóthRita Ungai-SalánkiBálint SzabóPéter FürjesJózsef PrechlNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Éva Sautner
Krisztián Papp
Eszter Holczer
Eszter L. Tóth
Rita Ungai-Salánki
Bálint Szabó
Péter Fürjes
József Prechl
Detection of red blood cell surface antigens by probe-triggered cell collision and flow retardation in an autonomous microfluidic system
description Abstract Microfluidic devices exploit combined physical, chemical and biological phenomena that could be unique in the sub-millimeter dimensions. The current goal of development of Point-of-Care (POC) medical devices is to extract the biomedical information from the blood. We examined the characteristics of blood flow in autonomous microfluidic devices with the aim to realize sensitive detection of interactions between particulate elements of the blood and the appropriately modified surfaces of the system. As a model experiment we demonstrated the fast analysis of the AB0 blood group system. We observed that the accumulation of red blood cells immobilized on the capillary wall leads to increased lateral movement of the flowing cells, resulting in the overall selective deceleration of the red blood cell flow column compared to the plasma fraction. We showed that by monitoring the flow rate characteristics in capillaries coated with blood type reagents it is possible to identify red blood cell types. Analysis of hydrodynamic effects governing blood flow by Finite Element Method based modelling supported our observations. Our proof-of-concept results point to a novel direction in blood analysis in autonomous microfluidic systems and also provide the basis for the construction of a simple quantitative device for blood group determination.
format article
author Éva Sautner
Krisztián Papp
Eszter Holczer
Eszter L. Tóth
Rita Ungai-Salánki
Bálint Szabó
Péter Fürjes
József Prechl
author_facet Éva Sautner
Krisztián Papp
Eszter Holczer
Eszter L. Tóth
Rita Ungai-Salánki
Bálint Szabó
Péter Fürjes
József Prechl
author_sort Éva Sautner
title Detection of red blood cell surface antigens by probe-triggered cell collision and flow retardation in an autonomous microfluidic system
title_short Detection of red blood cell surface antigens by probe-triggered cell collision and flow retardation in an autonomous microfluidic system
title_full Detection of red blood cell surface antigens by probe-triggered cell collision and flow retardation in an autonomous microfluidic system
title_fullStr Detection of red blood cell surface antigens by probe-triggered cell collision and flow retardation in an autonomous microfluidic system
title_full_unstemmed Detection of red blood cell surface antigens by probe-triggered cell collision and flow retardation in an autonomous microfluidic system
title_sort detection of red blood cell surface antigens by probe-triggered cell collision and flow retardation in an autonomous microfluidic system
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/2830640cbe9a4312afb7894c52235bdc
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