Garlic (Allium sativum)-derived SEVs inhibit cancer cell proliferation and induce caspase mediated apoptosis

Abstract As a key component of the cell-to-cell communication, small extracellular vesicles (SEVs) released from various sources are known to be affecting the physiological conditions of the target cells. Although it has been suggested that edible plant-derived nanoparticles contributes to the cross...

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Autores principales:	İrem Özkan, Polen Koçak, Merve Yıldırım, Naz Ünsal, Hazal Yılmaz, Dilek Telci, Fikrettin Şahin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2835cebee2144b41ab16037d6d4a4795
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spelling	oai:doaj.org-article:2835cebee2144b41ab16037d6d4a47952021-12-02T17:03:50ZGarlic (Allium sativum)-derived SEVs inhibit cancer cell proliferation and induce caspase mediated apoptosis10.1038/s41598-021-93876-42045-2322https://doaj.org/article/2835cebee2144b41ab16037d6d4a47952021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93876-4https://doaj.org/toc/2045-2322Abstract As a key component of the cell-to-cell communication, small extracellular vesicles (SEVs) released from various sources are known to be affecting the physiological conditions of the target cells. Although it has been suggested that edible plant-derived nanoparticles contributes to the cross kingdom communication with the mammalian cells, the effect of these particles on cancer cell progression still needs a further exploration. Here, we isolated and then characterized garlic derived SEVs by nanoparticle tracking analysis, electron microscopy and SEV surface antibodies. In order to investigate anti-cancer property of garlic SEVs A498 human kidney carcinoma, A549 human lung carcinoma were used as cell models along with the normal human dermal fibroblast cell lines. Annexin V/pI staining and analysis of apoptotic mRNA and protein expression levels suggested that garlic SEVs induced apoptosis through activation of intrinsic pathway. Furthermore, angiogenic VEGF protein expression levels significantly decreased in response to SEVs treatment in cancer cells. Our results support that garlic derived SEVs could cause apoptotic cell death among cancer cells while normal cells remain unaffected with the treatment. This study revealed for the first time that plant SEVs possess anti-cancer affects by inducing caspase mediated apoptosis and provided a new alternative for cancer treatment.İrem ÖzkanPolen KoçakMerve YıldırımNaz ÜnsalHazal YılmazDilek TelciFikrettin ŞahinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q İrem Özkan Polen Koçak Merve Yıldırım Naz Ünsal Hazal Yılmaz Dilek Telci Fikrettin Şahin Garlic (Allium sativum)-derived SEVs inhibit cancer cell proliferation and induce caspase mediated apoptosis
description	Abstract As a key component of the cell-to-cell communication, small extracellular vesicles (SEVs) released from various sources are known to be affecting the physiological conditions of the target cells. Although it has been suggested that edible plant-derived nanoparticles contributes to the cross kingdom communication with the mammalian cells, the effect of these particles on cancer cell progression still needs a further exploration. Here, we isolated and then characterized garlic derived SEVs by nanoparticle tracking analysis, electron microscopy and SEV surface antibodies. In order to investigate anti-cancer property of garlic SEVs A498 human kidney carcinoma, A549 human lung carcinoma were used as cell models along with the normal human dermal fibroblast cell lines. Annexin V/pI staining and analysis of apoptotic mRNA and protein expression levels suggested that garlic SEVs induced apoptosis through activation of intrinsic pathway. Furthermore, angiogenic VEGF protein expression levels significantly decreased in response to SEVs treatment in cancer cells. Our results support that garlic derived SEVs could cause apoptotic cell death among cancer cells while normal cells remain unaffected with the treatment. This study revealed for the first time that plant SEVs possess anti-cancer affects by inducing caspase mediated apoptosis and provided a new alternative for cancer treatment.
format	article
author	İrem Özkan Polen Koçak Merve Yıldırım Naz Ünsal Hazal Yılmaz Dilek Telci Fikrettin Şahin
author_facet	İrem Özkan Polen Koçak Merve Yıldırım Naz Ünsal Hazal Yılmaz Dilek Telci Fikrettin Şahin
author_sort	İrem Özkan
title	Garlic (Allium sativum)-derived SEVs inhibit cancer cell proliferation and induce caspase mediated apoptosis
title_short	Garlic (Allium sativum)-derived SEVs inhibit cancer cell proliferation and induce caspase mediated apoptosis
title_full	Garlic (Allium sativum)-derived SEVs inhibit cancer cell proliferation and induce caspase mediated apoptosis
title_fullStr	Garlic (Allium sativum)-derived SEVs inhibit cancer cell proliferation and induce caspase mediated apoptosis
title_full_unstemmed	Garlic (Allium sativum)-derived SEVs inhibit cancer cell proliferation and induce caspase mediated apoptosis
title_sort	garlic (allium sativum)-derived sevs inhibit cancer cell proliferation and induce caspase mediated apoptosis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2835cebee2144b41ab16037d6d4a4795
work_keys_str_mv	AT iremozkan garlicalliumsativumderivedsevsinhibitcancercellproliferationandinducecaspasemediatedapoptosis AT polenkocak garlicalliumsativumderivedsevsinhibitcancercellproliferationandinducecaspasemediatedapoptosis AT merveyıldırım garlicalliumsativumderivedsevsinhibitcancercellproliferationandinducecaspasemediatedapoptosis AT nazunsal garlicalliumsativumderivedsevsinhibitcancercellproliferationandinducecaspasemediatedapoptosis AT hazalyılmaz garlicalliumsativumderivedsevsinhibitcancercellproliferationandinducecaspasemediatedapoptosis AT dilektelci garlicalliumsativumderivedsevsinhibitcancercellproliferationandinducecaspasemediatedapoptosis AT fikrettinsahin garlicalliumsativumderivedsevsinhibitcancercellproliferationandinducecaspasemediatedapoptosis
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Garlic (Allium sativum)-derived SEVs inhibit cancer cell proliferation and induce caspase mediated apoptosis

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