Metabolomics analysis of grains of wheat infected and noninfected with Tilletia controversa Kühn

Abstract Dwarf bunt caused by the pathogen Tilletia controversa Kühn is one of the most serious quarantine diseases of winter wheat. Metabolomics studies provide detailed information about the biochemical changes at the cell and tissue levels of plants. In the present study, a liquid chromatography/...

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Autores principales:	Zhaoyu Ren, Mingke Fang, Ghulam Muhae-Ud-Din, Haifeng Gao, Yazhen Yang, Taiguo Liu, Wanquan Chen, Li Gao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/28394a5339f34b3aade0dca14cca64e0
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spelling	oai:doaj.org-article:28394a5339f34b3aade0dca14cca64e02021-12-02T18:48:02ZMetabolomics analysis of grains of wheat infected and noninfected with Tilletia controversa Kühn10.1038/s41598-021-98283-32045-2322https://doaj.org/article/28394a5339f34b3aade0dca14cca64e02021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98283-3https://doaj.org/toc/2045-2322Abstract Dwarf bunt caused by the pathogen Tilletia controversa Kühn is one of the most serious quarantine diseases of winter wheat. Metabolomics studies provide detailed information about the biochemical changes at the cell and tissue levels of plants. In the present study, a liquid chromatography/mass spectrometry (LC/MS) metabolomics approach was used to investigate the changes in the grain metabolomics of infected and noninfected with T. controversa samples. PCA suggested that T. controversa-infected and noninfected samples were separated during the interaction. LC/MS analysis showed that 62 different metabolites were recorded in the grains, among which a total of 34 metabolites were upregulated and 28 metabolites were downregulated. Prostaglandins (PGs) and 9-hydroxyoctadecadienoic acids (9-HODEs) are fungal toxin-related substances, and their expression significantly increased in T. controversa-infected grains. Additionally, the concentrations of cucurbic acid and octadecatrienoic acid changed significantly after pathogen infection, which play a large role in plant defense. The eight different metabolic pathways activated during T. controversa and wheat plant interactions included phenylalanine metabolism, isoquinoline alkaloid biosynthesis, starch and sucrose metabolism, tyrosine metabolism, sphingolipid metabolism, arginine and proline metabolism, alanine, aspartate, and glutamate metabolism, and tryptophan metabolism. In conclusion, we found differences in the metabolic profiles of wheat grains after T. controversa infection. To our knowledge, this is the first study to evaluate the metabolites in wheat grains after T. controversa infection.Zhaoyu RenMingke FangGhulam Muhae-Ud-DinHaifeng GaoYazhen YangTaiguo LiuWanquan ChenLi GaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhaoyu Ren Mingke Fang Ghulam Muhae-Ud-Din Haifeng Gao Yazhen Yang Taiguo Liu Wanquan Chen Li Gao Metabolomics analysis of grains of wheat infected and noninfected with Tilletia controversa Kühn
description	Abstract Dwarf bunt caused by the pathogen Tilletia controversa Kühn is one of the most serious quarantine diseases of winter wheat. Metabolomics studies provide detailed information about the biochemical changes at the cell and tissue levels of plants. In the present study, a liquid chromatography/mass spectrometry (LC/MS) metabolomics approach was used to investigate the changes in the grain metabolomics of infected and noninfected with T. controversa samples. PCA suggested that T. controversa-infected and noninfected samples were separated during the interaction. LC/MS analysis showed that 62 different metabolites were recorded in the grains, among which a total of 34 metabolites were upregulated and 28 metabolites were downregulated. Prostaglandins (PGs) and 9-hydroxyoctadecadienoic acids (9-HODEs) are fungal toxin-related substances, and their expression significantly increased in T. controversa-infected grains. Additionally, the concentrations of cucurbic acid and octadecatrienoic acid changed significantly after pathogen infection, which play a large role in plant defense. The eight different metabolic pathways activated during T. controversa and wheat plant interactions included phenylalanine metabolism, isoquinoline alkaloid biosynthesis, starch and sucrose metabolism, tyrosine metabolism, sphingolipid metabolism, arginine and proline metabolism, alanine, aspartate, and glutamate metabolism, and tryptophan metabolism. In conclusion, we found differences in the metabolic profiles of wheat grains after T. controversa infection. To our knowledge, this is the first study to evaluate the metabolites in wheat grains after T. controversa infection.
format	article
author	Zhaoyu Ren Mingke Fang Ghulam Muhae-Ud-Din Haifeng Gao Yazhen Yang Taiguo Liu Wanquan Chen Li Gao
author_facet	Zhaoyu Ren Mingke Fang Ghulam Muhae-Ud-Din Haifeng Gao Yazhen Yang Taiguo Liu Wanquan Chen Li Gao
author_sort	Zhaoyu Ren
title	Metabolomics analysis of grains of wheat infected and noninfected with Tilletia controversa Kühn
title_short	Metabolomics analysis of grains of wheat infected and noninfected with Tilletia controversa Kühn
title_full	Metabolomics analysis of grains of wheat infected and noninfected with Tilletia controversa Kühn
title_fullStr	Metabolomics analysis of grains of wheat infected and noninfected with Tilletia controversa Kühn
title_full_unstemmed	Metabolomics analysis of grains of wheat infected and noninfected with Tilletia controversa Kühn
title_sort	metabolomics analysis of grains of wheat infected and noninfected with tilletia controversa kühn
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/28394a5339f34b3aade0dca14cca64e0
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Metabolomics analysis of grains of wheat infected and noninfected with Tilletia controversa Kühn

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