Matrix-Bound Zolzoledronate Enhances the Biofilm Colonization of Hydroxyapatite: Effects on Osteonecrosis

(1) Background: The aim of this study was to test whether matrix-bound zoledronate (zol) molecules enhanced the oral biofilm colonization of a mineralized matrix, rendering the alveolar bone more susceptible to medication-related osteonecrosis of the jaw (MRONJ) following invasive dental procedures....

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Autores principales:	Ranya Elsayed, Ahmed El-Awady, Christopher Cutler, Zoya Kurago, Mahmoud Elashiry, Christina Sun, Ryan Bloomquist, Mohamed M. Meghil, Mohammed E. Elsalanty
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	osteonecrosis oral bacteria biofilm MRONJ osteoporosis bisphosphonates Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/284035c876e84fce95d2ccc18161bbfa
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spelling	oai:doaj.org-article:284035c876e84fce95d2ccc18161bbfa2021-11-25T16:24:22ZMatrix-Bound Zolzoledronate Enhances the Biofilm Colonization of Hydroxyapatite: Effects on Osteonecrosis10.3390/antibiotics101113802079-6382https://doaj.org/article/284035c876e84fce95d2ccc18161bbfa2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1380https://doaj.org/toc/2079-6382(1) Background: The aim of this study was to test whether matrix-bound zoledronate (zol) molecules enhanced the oral biofilm colonization of a mineralized matrix, rendering the alveolar bone more susceptible to medication-related osteonecrosis of the jaw (MRONJ) following invasive dental procedures. (2) Methods: We tested the effect of matrix-bound zol on the growth and attachment of <i>Porphyromonas gingivalis</i> (Pg), <i>Fusobacterium nucleatum</i> (Fn) and <i>Actinomyces israelii</i> (Ai), and whether the nitrogen-containing component of zol contributed to such effect. The role of oral bacteria in the induction of osteonecrosis was then tested using an extra-oral bone defect model. (3) Results: The attachment of biofilm to hydroxyapatite discs increased when the discs were pre-treated with zol. Bacterial proliferation was not affected. Matrix-bound zol was more potent than non-nitrogen-containing etidronate in enhancing the colonization. Stimulation was dampened by pre-treating the bacteria with histidine. The delivery of oral biofilm to a tibial defect caused osteonecrosis in zol-treated rats. (4) Conclusions: We conclude that matrix-bound zol enhances the oral biofilm colonization of hydroxyapatite. This enhancement depended on the presence of the nitrogen-containing group. The oral biofilm rendered the extra-oral bone susceptible to medication-related osteonecrosis, suggesting that it has an important role in the induction of MRONJ.Ranya ElsayedAhmed El-AwadyChristopher CutlerZoya KuragoMahmoud ElashiryChristina SunRyan BloomquistMohamed M. MeghilMohammed E. ElsalantyMDPI AGarticleosteonecrosisoral bacteriabiofilmMRONJosteoporosisbisphosphonatesTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1380, p 1380 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	osteonecrosis oral bacteria biofilm MRONJ osteoporosis bisphosphonates Therapeutics. Pharmacology RM1-950
spellingShingle	osteonecrosis oral bacteria biofilm MRONJ osteoporosis bisphosphonates Therapeutics. Pharmacology RM1-950 Ranya Elsayed Ahmed El-Awady Christopher Cutler Zoya Kurago Mahmoud Elashiry Christina Sun Ryan Bloomquist Mohamed M. Meghil Mohammed E. Elsalanty Matrix-Bound Zolzoledronate Enhances the Biofilm Colonization of Hydroxyapatite: Effects on Osteonecrosis
description	(1) Background: The aim of this study was to test whether matrix-bound zoledronate (zol) molecules enhanced the oral biofilm colonization of a mineralized matrix, rendering the alveolar bone more susceptible to medication-related osteonecrosis of the jaw (MRONJ) following invasive dental procedures. (2) Methods: We tested the effect of matrix-bound zol on the growth and attachment of <i>Porphyromonas gingivalis</i> (Pg), <i>Fusobacterium nucleatum</i> (Fn) and <i>Actinomyces israelii</i> (Ai), and whether the nitrogen-containing component of zol contributed to such effect. The role of oral bacteria in the induction of osteonecrosis was then tested using an extra-oral bone defect model. (3) Results: The attachment of biofilm to hydroxyapatite discs increased when the discs were pre-treated with zol. Bacterial proliferation was not affected. Matrix-bound zol was more potent than non-nitrogen-containing etidronate in enhancing the colonization. Stimulation was dampened by pre-treating the bacteria with histidine. The delivery of oral biofilm to a tibial defect caused osteonecrosis in zol-treated rats. (4) Conclusions: We conclude that matrix-bound zol enhances the oral biofilm colonization of hydroxyapatite. This enhancement depended on the presence of the nitrogen-containing group. The oral biofilm rendered the extra-oral bone susceptible to medication-related osteonecrosis, suggesting that it has an important role in the induction of MRONJ.
format	article
author	Ranya Elsayed Ahmed El-Awady Christopher Cutler Zoya Kurago Mahmoud Elashiry Christina Sun Ryan Bloomquist Mohamed M. Meghil Mohammed E. Elsalanty
author_facet	Ranya Elsayed Ahmed El-Awady Christopher Cutler Zoya Kurago Mahmoud Elashiry Christina Sun Ryan Bloomquist Mohamed M. Meghil Mohammed E. Elsalanty
author_sort	Ranya Elsayed
title	Matrix-Bound Zolzoledronate Enhances the Biofilm Colonization of Hydroxyapatite: Effects on Osteonecrosis
title_short	Matrix-Bound Zolzoledronate Enhances the Biofilm Colonization of Hydroxyapatite: Effects on Osteonecrosis
title_full	Matrix-Bound Zolzoledronate Enhances the Biofilm Colonization of Hydroxyapatite: Effects on Osteonecrosis
title_fullStr	Matrix-Bound Zolzoledronate Enhances the Biofilm Colonization of Hydroxyapatite: Effects on Osteonecrosis
title_full_unstemmed	Matrix-Bound Zolzoledronate Enhances the Biofilm Colonization of Hydroxyapatite: Effects on Osteonecrosis
title_sort	matrix-bound zolzoledronate enhances the biofilm colonization of hydroxyapatite: effects on osteonecrosis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/284035c876e84fce95d2ccc18161bbfa
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Matrix-Bound Zolzoledronate Enhances the Biofilm Colonization of Hydroxyapatite: Effects on Osteonecrosis

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