uvCLAP is a fast and non-radioactive method to identify in vivo targets of RNA-binding proteins

RNA-binding proteins have important roles in gene expression and regulation. Here the authors develop uvCLAP to purify proteins and determine their binding sites without the use of radioactivity.

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Detalles Bibliográficos
Autores principales: Daniel Maticzka, Ibrahim Avsar Ilik, Tugce Aktas, Rolf Backofen, Asifa Akhtar
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/28437366da674ac9985b0fd4e479430d
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Descripción
Sumario:RNA-binding proteins have important roles in gene expression and regulation. Here the authors develop uvCLAP to purify proteins and determine their binding sites without the use of radioactivity.