uvCLAP is a fast and non-radioactive method to identify in vivo targets of RNA-binding proteins

RNA-binding proteins have important roles in gene expression and regulation. Here the authors develop uvCLAP to purify proteins and determine their binding sites without the use of radioactivity.

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Autores principales: Daniel Maticzka, Ibrahim Avsar Ilik, Tugce Aktas, Rolf Backofen, Asifa Akhtar
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/28437366da674ac9985b0fd4e479430d
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spelling oai:doaj.org-article:28437366da674ac9985b0fd4e479430d2021-12-02T15:34:04ZuvCLAP is a fast and non-radioactive method to identify in vivo targets of RNA-binding proteins10.1038/s41467-018-03575-42041-1723https://doaj.org/article/28437366da674ac9985b0fd4e479430d2018-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-03575-4https://doaj.org/toc/2041-1723RNA-binding proteins have important roles in gene expression and regulation. Here the authors develop uvCLAP to purify proteins and determine their binding sites without the use of radioactivity.Daniel MaticzkaIbrahim Avsar IlikTugce AktasRolf BackofenAsifa AkhtarNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-13 (2018)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Daniel Maticzka
Ibrahim Avsar Ilik
Tugce Aktas
Rolf Backofen
Asifa Akhtar
uvCLAP is a fast and non-radioactive method to identify in vivo targets of RNA-binding proteins
description RNA-binding proteins have important roles in gene expression and regulation. Here the authors develop uvCLAP to purify proteins and determine their binding sites without the use of radioactivity.
format article
author Daniel Maticzka
Ibrahim Avsar Ilik
Tugce Aktas
Rolf Backofen
Asifa Akhtar
author_facet Daniel Maticzka
Ibrahim Avsar Ilik
Tugce Aktas
Rolf Backofen
Asifa Akhtar
author_sort Daniel Maticzka
title uvCLAP is a fast and non-radioactive method to identify in vivo targets of RNA-binding proteins
title_short uvCLAP is a fast and non-radioactive method to identify in vivo targets of RNA-binding proteins
title_full uvCLAP is a fast and non-radioactive method to identify in vivo targets of RNA-binding proteins
title_fullStr uvCLAP is a fast and non-radioactive method to identify in vivo targets of RNA-binding proteins
title_full_unstemmed uvCLAP is a fast and non-radioactive method to identify in vivo targets of RNA-binding proteins
title_sort uvclap is a fast and non-radioactive method to identify in vivo targets of rna-binding proteins
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/28437366da674ac9985b0fd4e479430d
work_keys_str_mv AT danielmaticzka uvclapisafastandnonradioactivemethodtoidentifyinvivotargetsofrnabindingproteins
AT ibrahimavsarilik uvclapisafastandnonradioactivemethodtoidentifyinvivotargetsofrnabindingproteins
AT tugceaktas uvclapisafastandnonradioactivemethodtoidentifyinvivotargetsofrnabindingproteins
AT rolfbackofen uvclapisafastandnonradioactivemethodtoidentifyinvivotargetsofrnabindingproteins
AT asifaakhtar uvclapisafastandnonradioactivemethodtoidentifyinvivotargetsofrnabindingproteins
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