Saturation transfer MRI is sensitive to neurochemical changes in the rat brain due to chronic unpredictable mild stress

Abstract Chemical exchange saturation transfer (CEST) MRI was performed for the evaluation of cerebral metabolic changes in a rat model of depressive-like disease induced by chronic unpredictable mild stress (CUMS). CEST Z-spectra were acquired on a 7 T MRI with two saturation B1 amplitudes (0.5 and...

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Autores principales:	Anna Pankowska, Agata Chudzik, Tymoteusz Słowik, Artur Łazorczyk, Katarzyna Kochalska, Marta Andres-Mach, Wilfred W. Lam, Radosław Pietura, Radosław Rola, Greg J. Stanisz, Anna Orzyłowska
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2843abc3fa2d439987292d7819922fdb
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spelling	oai:doaj.org-article:2843abc3fa2d439987292d7819922fdb2021-12-02T18:14:08ZSaturation transfer MRI is sensitive to neurochemical changes in the rat brain due to chronic unpredictable mild stress10.1038/s41598-021-97991-02045-2322https://doaj.org/article/2843abc3fa2d439987292d7819922fdb2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97991-0https://doaj.org/toc/2045-2322Abstract Chemical exchange saturation transfer (CEST) MRI was performed for the evaluation of cerebral metabolic changes in a rat model of depressive-like disease induced by chronic unpredictable mild stress (CUMS). CEST Z-spectra were acquired on a 7 T MRI with two saturation B1 amplitudes (0.5 and 0.75 µT) to measure the magnetization transfer ratio (MTR), CEST and relayed nuclear Overhauser effect (rNOE). Cerebral cortex and hippocampus were examined in two groups of animals: healthy control (n = 10) and stressed (n = 14), the latter of which was exposed to eight weeks of the CUMS protocol. The stressed group Z-spectrum parameters, primarily MTRs, were significantly lower than in controls, at all selected frequency offsets (3.5, 3.0, 2.0, − 3.2, − 3.6 ppm) in the cortex (the largest difference of ~ 3.5% at − 3.6 ppm, p = 0.0005) and the hippocampus (MTRs measured with a B1 = 0.5 µT). The hippocampal rNOE contributions decreased significantly in the stressed brains. Glutamate concentration (assessed using ELISA) and MTR at 3 ppm correlated positively in both brain regions. GABA concentration also correlated positively with CEST contributions in both cerebral areas, while such correlation with MTR was positive in hippocampus, and nonsignificant in cortex. Results indicate that CEST is sensitive to neurometabolic changes following chronic stress exposure.Anna PankowskaAgata ChudzikTymoteusz SłowikArtur ŁazorczykKatarzyna KochalskaMarta Andres-MachWilfred W. LamRadosław PieturaRadosław RolaGreg J. StaniszAnna OrzyłowskaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna Pankowska Agata Chudzik Tymoteusz Słowik Artur Łazorczyk Katarzyna Kochalska Marta Andres-Mach Wilfred W. Lam Radosław Pietura Radosław Rola Greg J. Stanisz Anna Orzyłowska Saturation transfer MRI is sensitive to neurochemical changes in the rat brain due to chronic unpredictable mild stress
description	Abstract Chemical exchange saturation transfer (CEST) MRI was performed for the evaluation of cerebral metabolic changes in a rat model of depressive-like disease induced by chronic unpredictable mild stress (CUMS). CEST Z-spectra were acquired on a 7 T MRI with two saturation B1 amplitudes (0.5 and 0.75 µT) to measure the magnetization transfer ratio (MTR), CEST and relayed nuclear Overhauser effect (rNOE). Cerebral cortex and hippocampus were examined in two groups of animals: healthy control (n = 10) and stressed (n = 14), the latter of which was exposed to eight weeks of the CUMS protocol. The stressed group Z-spectrum parameters, primarily MTRs, were significantly lower than in controls, at all selected frequency offsets (3.5, 3.0, 2.0, − 3.2, − 3.6 ppm) in the cortex (the largest difference of ~ 3.5% at − 3.6 ppm, p = 0.0005) and the hippocampus (MTRs measured with a B1 = 0.5 µT). The hippocampal rNOE contributions decreased significantly in the stressed brains. Glutamate concentration (assessed using ELISA) and MTR at 3 ppm correlated positively in both brain regions. GABA concentration also correlated positively with CEST contributions in both cerebral areas, while such correlation with MTR was positive in hippocampus, and nonsignificant in cortex. Results indicate that CEST is sensitive to neurometabolic changes following chronic stress exposure.
format	article
author	Anna Pankowska Agata Chudzik Tymoteusz Słowik Artur Łazorczyk Katarzyna Kochalska Marta Andres-Mach Wilfred W. Lam Radosław Pietura Radosław Rola Greg J. Stanisz Anna Orzyłowska
author_facet	Anna Pankowska Agata Chudzik Tymoteusz Słowik Artur Łazorczyk Katarzyna Kochalska Marta Andres-Mach Wilfred W. Lam Radosław Pietura Radosław Rola Greg J. Stanisz Anna Orzyłowska
author_sort	Anna Pankowska
title	Saturation transfer MRI is sensitive to neurochemical changes in the rat brain due to chronic unpredictable mild stress
title_short	Saturation transfer MRI is sensitive to neurochemical changes in the rat brain due to chronic unpredictable mild stress
title_full	Saturation transfer MRI is sensitive to neurochemical changes in the rat brain due to chronic unpredictable mild stress
title_fullStr	Saturation transfer MRI is sensitive to neurochemical changes in the rat brain due to chronic unpredictable mild stress
title_full_unstemmed	Saturation transfer MRI is sensitive to neurochemical changes in the rat brain due to chronic unpredictable mild stress
title_sort	saturation transfer mri is sensitive to neurochemical changes in the rat brain due to chronic unpredictable mild stress
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2843abc3fa2d439987292d7819922fdb
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Saturation transfer MRI is sensitive to neurochemical changes in the rat brain due to chronic unpredictable mild stress

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