Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale

Combining improved sample preparation, data-independent acquisition mass spectrometry and deep learning, the authors develop a workflow for more robust and precise quantitative ubiquitinome profiling. They use this method to characterize targets of the deubiquitinase USP7 and effects of USP7 inhibit...

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Autores principales:	Martin Steger, Vadim Demichev, Mattias Backman, Uli Ohmayer, Phillip Ihmor, Stefan Müller, Markus Ralser, Henrik Daub
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/284693f5e9d14df9b7b8bbbd91efb49e
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spelling	oai:doaj.org-article:284693f5e9d14df9b7b8bbbd91efb49e2021-12-02T18:50:53ZTime-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale10.1038/s41467-021-25454-12041-1723https://doaj.org/article/284693f5e9d14df9b7b8bbbd91efb49e2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25454-1https://doaj.org/toc/2041-1723Combining improved sample preparation, data-independent acquisition mass spectrometry and deep learning, the authors develop a workflow for more robust and precise quantitative ubiquitinome profiling. They use this method to characterize targets of the deubiquitinase USP7 and effects of USP7 inhibitors.Martin StegerVadim DemichevMattias BackmanUli OhmayerPhillip IhmorStefan MüllerMarkus RalserHenrik DaubNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Martin Steger Vadim Demichev Mattias Backman Uli Ohmayer Phillip Ihmor Stefan Müller Markus Ralser Henrik Daub Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale
description	Combining improved sample preparation, data-independent acquisition mass spectrometry and deep learning, the authors develop a workflow for more robust and precise quantitative ubiquitinome profiling. They use this method to characterize targets of the deubiquitinase USP7 and effects of USP7 inhibitors.
format	article
author	Martin Steger Vadim Demichev Mattias Backman Uli Ohmayer Phillip Ihmor Stefan Müller Markus Ralser Henrik Daub
author_facet	Martin Steger Vadim Demichev Mattias Backman Uli Ohmayer Phillip Ihmor Stefan Müller Markus Ralser Henrik Daub
author_sort	Martin Steger
title	Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale
title_short	Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale
title_full	Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale
title_fullStr	Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale
title_full_unstemmed	Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale
title_sort	time-resolved in vivo ubiquitinome profiling by dia-ms reveals usp7 targets on a proteome-wide scale
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/284693f5e9d14df9b7b8bbbd91efb49e
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Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale

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