Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale

Combining improved sample preparation, data-independent acquisition mass spectrometry and deep learning, the authors develop a workflow for more robust and precise quantitative ubiquitinome profiling. They use this method to characterize targets of the deubiquitinase USP7 and effects of USP7 inhibit...

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Bibliographic Details
Main Authors: Martin Steger, Vadim Demichev, Mattias Backman, Uli Ohmayer, Phillip Ihmor, Stefan Müller, Markus Ralser, Henrik Daub
Format: article
Language:EN
Published: Nature Portfolio 2021
Subjects:
Q
Online Access:https://doaj.org/article/284693f5e9d14df9b7b8bbbd91efb49e
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