A novel mouse line with epididymal initial segment-specific expression of Cre recombinase driven by the endogenous Lcn9 promoter.

Spermatozoa released from testes undergo a maturation process and acquire the capacity to fertilize ova through epididymal transit. The epididymis is divided into four regions, each with unique morphology, gene profile, luminal microenvironment and distinct function. To study the functions of releva...

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Autores principales:	Qian-Qian Gong, Xiao Wang, Zhi-Lin Dou, Ke-Yi Zhang, Xiang-Guo Liu, Jian-Gang Gao, Xiao-Yang Sun
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2847a762b6b0438f933b7d1aabe9c16a
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spelling	oai:doaj.org-article:2847a762b6b0438f933b7d1aabe9c16a2021-12-02T20:04:50ZA novel mouse line with epididymal initial segment-specific expression of Cre recombinase driven by the endogenous Lcn9 promoter.1932-620310.1371/journal.pone.0254802https://doaj.org/article/2847a762b6b0438f933b7d1aabe9c16a2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254802https://doaj.org/toc/1932-6203Spermatozoa released from testes undergo a maturation process and acquire the capacity to fertilize ova through epididymal transit. The epididymis is divided into four regions, each with unique morphology, gene profile, luminal microenvironment and distinct function. To study the functions of relevant genes in the epididymal initial segment (IS), a novel IS-specific mouse model, Lcn9-Cre knock-in (KI) mouse line was generated via CRISPR/Cas9 technology. The TAG stop codon was replaced by a 2A-NLS-Cre cassette, resulting in the co-expression of Lcn9 and Cre recombinase. IS-specific Cre expression was first observed from postnatal day 17. Using the Rosa26tdTomato reporter mice, the Cre-mediated DNA recombination was detected exclusively in principal cells. The epididymal IS-specific Cre activity in vivo was further confirmed using Lcn9-Cre mice crossed with a mouse strain carrying Tsc1 floxed alleles (Tsc1flox/+). Cre expression did not affect either normal development or male fecundity. Different from any epididymis-specific Cre mice reported previously, the novel Lcn9-Cre mouse line can be used to introduce entire IS-specific conditional gene editing for gene functional study.Qian-Qian GongQian-Qian GongXiao WangZhi-Lin DouKe-Yi ZhangXiang-Guo LiuJian-Gang GaoXiao-Yang SunPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254802 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qian-Qian Gong Qian-Qian Gong Xiao Wang Zhi-Lin Dou Ke-Yi Zhang Xiang-Guo Liu Jian-Gang Gao Xiao-Yang Sun A novel mouse line with epididymal initial segment-specific expression of Cre recombinase driven by the endogenous Lcn9 promoter.
description	Spermatozoa released from testes undergo a maturation process and acquire the capacity to fertilize ova through epididymal transit. The epididymis is divided into four regions, each with unique morphology, gene profile, luminal microenvironment and distinct function. To study the functions of relevant genes in the epididymal initial segment (IS), a novel IS-specific mouse model, Lcn9-Cre knock-in (KI) mouse line was generated via CRISPR/Cas9 technology. The TAG stop codon was replaced by a 2A-NLS-Cre cassette, resulting in the co-expression of Lcn9 and Cre recombinase. IS-specific Cre expression was first observed from postnatal day 17. Using the Rosa26tdTomato reporter mice, the Cre-mediated DNA recombination was detected exclusively in principal cells. The epididymal IS-specific Cre activity in vivo was further confirmed using Lcn9-Cre mice crossed with a mouse strain carrying Tsc1 floxed alleles (Tsc1flox/+). Cre expression did not affect either normal development or male fecundity. Different from any epididymis-specific Cre mice reported previously, the novel Lcn9-Cre mouse line can be used to introduce entire IS-specific conditional gene editing for gene functional study.
format	article
author	Qian-Qian Gong Qian-Qian Gong Xiao Wang Zhi-Lin Dou Ke-Yi Zhang Xiang-Guo Liu Jian-Gang Gao Xiao-Yang Sun
author_facet	Qian-Qian Gong Qian-Qian Gong Xiao Wang Zhi-Lin Dou Ke-Yi Zhang Xiang-Guo Liu Jian-Gang Gao Xiao-Yang Sun
author_sort	Qian-Qian Gong
title	A novel mouse line with epididymal initial segment-specific expression of Cre recombinase driven by the endogenous Lcn9 promoter.
title_short	A novel mouse line with epididymal initial segment-specific expression of Cre recombinase driven by the endogenous Lcn9 promoter.
title_full	A novel mouse line with epididymal initial segment-specific expression of Cre recombinase driven by the endogenous Lcn9 promoter.
title_fullStr	A novel mouse line with epididymal initial segment-specific expression of Cre recombinase driven by the endogenous Lcn9 promoter.
title_full_unstemmed	A novel mouse line with epididymal initial segment-specific expression of Cre recombinase driven by the endogenous Lcn9 promoter.
title_sort	novel mouse line with epididymal initial segment-specific expression of cre recombinase driven by the endogenous lcn9 promoter.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/2847a762b6b0438f933b7d1aabe9c16a
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A novel mouse line with epididymal initial segment-specific expression of Cre recombinase driven by the endogenous Lcn9 promoter.

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