Spider Toxin Peptide-Induced NIR Gold Nanocluster Fabrication for GSH-Responsive Cancer Cell Imaging and Nuclei Translocation

Goldnanoclusters (GNCs) have become a promising nanomaterial for bioimaging because of their unique optical properties and biocompatibility. In this study, lycosin-I peptide, which possesses a highly selective anticancer activity by affecting the permeability of cancer cell membrane, was firstly mod...

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Autores principales: Huaxin Tan, Sisi Liu, Yaolin He, Guofeng Cheng, Yu Zhang, Xiaojie Wei, Lidan Hu
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Lenguaje:EN
Publicado: Frontiers Media S.A. 2021
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Acceso en línea:https://doaj.org/article/284a539f86ae48f19b40739a522ab656
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spelling oai:doaj.org-article:284a539f86ae48f19b40739a522ab6562021-11-16T07:33:54ZSpider Toxin Peptide-Induced NIR Gold Nanocluster Fabrication for GSH-Responsive Cancer Cell Imaging and Nuclei Translocation2296-418510.3389/fbioe.2021.780223https://doaj.org/article/284a539f86ae48f19b40739a522ab6562021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.780223/fullhttps://doaj.org/toc/2296-4185Goldnanoclusters (GNCs) have become a promising nanomaterial for bioimaging because of their unique optical properties and biocompatibility. In this study, lycosin-I peptide, which possesses a highly selective anticancer activity by affecting the permeability of cancer cell membrane, was firstly modified for constructing fluorescent GNCs (LGNCs) for bioimaging of tumor cells. The obtained LGNCs exhibited strong near-infrared (NIR) fluorescence, which can be further enhanced by the peptide-induced aggregation and selectively stained three cancerous cell lines over normal cell lines with low intrinsic toxicity. After uptake by tumor cells, LGNC aggregates can be depolymerized into ultrasmall nanoclusters by high-level glutathione (GSH) and realize the nuclear targeting translocation. Collectively, our work suggests the potential of natural active biomolecules in designing NIR fluorescent GNCs for bioimaging.Huaxin TanSisi LiuYaolin HeGuofeng ChengYu ZhangXiaojie WeiLidan HuFrontiers Media S.A.articleNIR gold nanoclustersspider toxin peptidecancer cell imagingnuclei translocationGSH-responsiveBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution DOAJ
collection DOAJ
language EN
topic NIR gold nanoclusters
spider toxin peptide
cancer cell imaging
nuclei translocation
GSH-responsive
Biotechnology
TP248.13-248.65
spellingShingle NIR gold nanoclusters
spider toxin peptide
cancer cell imaging
nuclei translocation
GSH-responsive
Biotechnology
TP248.13-248.65
Huaxin Tan
Sisi Liu
Yaolin He
Guofeng Cheng
Yu Zhang
Xiaojie Wei
Lidan Hu
Spider Toxin Peptide-Induced NIR Gold Nanocluster Fabrication for GSH-Responsive Cancer Cell Imaging and Nuclei Translocation
description Goldnanoclusters (GNCs) have become a promising nanomaterial for bioimaging because of their unique optical properties and biocompatibility. In this study, lycosin-I peptide, which possesses a highly selective anticancer activity by affecting the permeability of cancer cell membrane, was firstly modified for constructing fluorescent GNCs (LGNCs) for bioimaging of tumor cells. The obtained LGNCs exhibited strong near-infrared (NIR) fluorescence, which can be further enhanced by the peptide-induced aggregation and selectively stained three cancerous cell lines over normal cell lines with low intrinsic toxicity. After uptake by tumor cells, LGNC aggregates can be depolymerized into ultrasmall nanoclusters by high-level glutathione (GSH) and realize the nuclear targeting translocation. Collectively, our work suggests the potential of natural active biomolecules in designing NIR fluorescent GNCs for bioimaging.
format article
author Huaxin Tan
Sisi Liu
Yaolin He
Guofeng Cheng
Yu Zhang
Xiaojie Wei
Lidan Hu
author_facet Huaxin Tan
Sisi Liu
Yaolin He
Guofeng Cheng
Yu Zhang
Xiaojie Wei
Lidan Hu
author_sort Huaxin Tan
title Spider Toxin Peptide-Induced NIR Gold Nanocluster Fabrication for GSH-Responsive Cancer Cell Imaging and Nuclei Translocation
title_short Spider Toxin Peptide-Induced NIR Gold Nanocluster Fabrication for GSH-Responsive Cancer Cell Imaging and Nuclei Translocation
title_full Spider Toxin Peptide-Induced NIR Gold Nanocluster Fabrication for GSH-Responsive Cancer Cell Imaging and Nuclei Translocation
title_fullStr Spider Toxin Peptide-Induced NIR Gold Nanocluster Fabrication for GSH-Responsive Cancer Cell Imaging and Nuclei Translocation
title_full_unstemmed Spider Toxin Peptide-Induced NIR Gold Nanocluster Fabrication for GSH-Responsive Cancer Cell Imaging and Nuclei Translocation
title_sort spider toxin peptide-induced nir gold nanocluster fabrication for gsh-responsive cancer cell imaging and nuclei translocation
publisher Frontiers Media S.A.
publishDate 2021
url https://doaj.org/article/284a539f86ae48f19b40739a522ab656
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