Modulation of fat metabolism and gut microbiota by resveratrol on high-fat diet-induced obese mice

C Linda Campbell,1,2,* Renqiang Yu,3,4 Fengzhi Li,1 Qin Zhou,3 Daozhen Chen,4 Ce Qi,1,2 Yongxiang Yin,5 Jin Sun1,2,* 1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; 2School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; 3Depart...

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Autores principales:	Campbell CL, Yu R, Li F, Zhou Q, Chen D, Qi C, Yin Y, Sun J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Resveratrol High fat diet obesity oxidative stress gut microbiota Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/285274a79f564c88a4b5426b51f72236
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spelling	oai:doaj.org-article:285274a79f564c88a4b5426b51f722362021-12-02T04:00:45ZModulation of fat metabolism and gut microbiota by resveratrol on high-fat diet-induced obese mice1178-7007https://doaj.org/article/285274a79f564c88a4b5426b51f722362019-01-01T00:00:00Zhttps://www.dovepress.com/modulation-of-fat-metabolism-and-gut-microbiota-by-resveratrol-on-high-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007C Linda Campbell,1,2,* Renqiang Yu,3,4 Fengzhi Li,1 Qin Zhou,3 Daozhen Chen,4 Ce Qi,1,2 Yongxiang Yin,5 Jin Sun1,2,* 1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; 2School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; 3Department of Neonatology, The Affiliated Wuxi Maternity and Child Health Care Hospital of Nanjing Medical University, Wuxi 214002, China; 4Department of Central Lab, The Affiliated Wuxi Maternity and Child Health Care Hospital of Nanjing Medical University, Wuxi 214002, China; 5Department of Pathology, The Affiliated Wuxi Maternity and Child Health Care Hospital of Nanjing Medical University, Wuxi 214002, China *These authors contributed equally to this work Purpose: The antioxidant resveratrol (RSV) has low bioavailability and can reach the colon to access the gut microbial ecosystem. RSV administration together with high-fat diet prevented abnormal changes of intestinal microbiota. However, whether or not RSV can reshape the intestinal microbiota of obese mice and alleviate obesity-related diseases remains to be studied. This study aimed to explore the role of RSV in alleviating high-fat-induced obesity and its relationship with oxidative stress and gut microbiota.Methods: Male C57BL/6 mice were divided into five groups and administered for 16 weeks with: standard diet (CON), high-fat diet (60% energy for lard, HFD), and HFD with low, medium, and high dose of RSV, 50, 75, and 100 mg/kg body weight administered daily via drinking water, respectively.Results: Medium and high RSV treatment significantly prevented body weight gain, decreased relative weight of liver and adipose tissue compared with HFD (P<0.05). All doses significantly prevented HFD-induced increase of serum triglyceride, low density lipoprotein cholesterol, glucose, and endotoxemia (P<0.05). Medium and high dose also prevented chronic inflammation by decreasing serum interleukin-1 and tumor necrosis factor-alpha (P<0.05), and oxidative stress in liver and brain indicated by increase in superoxide dismutase, catalase, glutathione peroxidase activity (P<0.05). Formation of malondialdehyde was prevented by all doses compared with HFD (P<0.05). Both medium and high doses of RES increased alpha diversity of gut microbiota according to the Chao1 and Shannon indices (P<0.05). Medium dose induced obvious shift in gut microbiota composition according to principal component analysis. High dose of RSV effectively prevented HFD-induced increase of Coriobacteriaceae and Desulfovibrionaceae (P<0.05), which show a significant correlation with body weight (r>0.8 P<0.00).Conclusion: RSV prevented HFD-induced endotoxemia, oxidative stress, and gut microbiota change. Keywords: resveratrol, high fat diet, obesity, oxidative stress, gut microbiotaCampbell CLYu RLi FZhou QChen DQi CYin YSun JDove Medical PressarticleResveratrolHigh fat dietobesityoxidative stressgut microbiotaSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 12, Pp 97-107 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Resveratrol High fat diet obesity oxidative stress gut microbiota Specialties of internal medicine RC581-951
spellingShingle	Resveratrol High fat diet obesity oxidative stress gut microbiota Specialties of internal medicine RC581-951 Campbell CL Yu R Li F Zhou Q Chen D Qi C Yin Y Sun J Modulation of fat metabolism and gut microbiota by resveratrol on high-fat diet-induced obese mice
description	C Linda Campbell,1,2,* Renqiang Yu,3,4 Fengzhi Li,1 Qin Zhou,3 Daozhen Chen,4 Ce Qi,1,2 Yongxiang Yin,5 Jin Sun1,2,* 1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; 2School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; 3Department of Neonatology, The Affiliated Wuxi Maternity and Child Health Care Hospital of Nanjing Medical University, Wuxi 214002, China; 4Department of Central Lab, The Affiliated Wuxi Maternity and Child Health Care Hospital of Nanjing Medical University, Wuxi 214002, China; 5Department of Pathology, The Affiliated Wuxi Maternity and Child Health Care Hospital of Nanjing Medical University, Wuxi 214002, China *These authors contributed equally to this work Purpose: The antioxidant resveratrol (RSV) has low bioavailability and can reach the colon to access the gut microbial ecosystem. RSV administration together with high-fat diet prevented abnormal changes of intestinal microbiota. However, whether or not RSV can reshape the intestinal microbiota of obese mice and alleviate obesity-related diseases remains to be studied. This study aimed to explore the role of RSV in alleviating high-fat-induced obesity and its relationship with oxidative stress and gut microbiota.Methods: Male C57BL/6 mice were divided into five groups and administered for 16 weeks with: standard diet (CON), high-fat diet (60% energy for lard, HFD), and HFD with low, medium, and high dose of RSV, 50, 75, and 100 mg/kg body weight administered daily via drinking water, respectively.Results: Medium and high RSV treatment significantly prevented body weight gain, decreased relative weight of liver and adipose tissue compared with HFD (P<0.05). All doses significantly prevented HFD-induced increase of serum triglyceride, low density lipoprotein cholesterol, glucose, and endotoxemia (P<0.05). Medium and high dose also prevented chronic inflammation by decreasing serum interleukin-1 and tumor necrosis factor-alpha (P<0.05), and oxidative stress in liver and brain indicated by increase in superoxide dismutase, catalase, glutathione peroxidase activity (P<0.05). Formation of malondialdehyde was prevented by all doses compared with HFD (P<0.05). Both medium and high doses of RES increased alpha diversity of gut microbiota according to the Chao1 and Shannon indices (P<0.05). Medium dose induced obvious shift in gut microbiota composition according to principal component analysis. High dose of RSV effectively prevented HFD-induced increase of Coriobacteriaceae and Desulfovibrionaceae (P<0.05), which show a significant correlation with body weight (r>0.8 P<0.00).Conclusion: RSV prevented HFD-induced endotoxemia, oxidative stress, and gut microbiota change. Keywords: resveratrol, high fat diet, obesity, oxidative stress, gut microbiota
format	article
author	Campbell CL Yu R Li F Zhou Q Chen D Qi C Yin Y Sun J
author_facet	Campbell CL Yu R Li F Zhou Q Chen D Qi C Yin Y Sun J
author_sort	Campbell CL
title	Modulation of fat metabolism and gut microbiota by resveratrol on high-fat diet-induced obese mice
title_short	Modulation of fat metabolism and gut microbiota by resveratrol on high-fat diet-induced obese mice
title_full	Modulation of fat metabolism and gut microbiota by resveratrol on high-fat diet-induced obese mice
title_fullStr	Modulation of fat metabolism and gut microbiota by resveratrol on high-fat diet-induced obese mice
title_full_unstemmed	Modulation of fat metabolism and gut microbiota by resveratrol on high-fat diet-induced obese mice
title_sort	modulation of fat metabolism and gut microbiota by resveratrol on high-fat diet-induced obese mice
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/285274a79f564c88a4b5426b51f72236
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Modulation of fat metabolism and gut microbiota by resveratrol on high-fat diet-induced obese mice

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