Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-β-synthase domain

Trypanosoma brucei guanosine 5′-monophosphate reductase (TbGMPR) catalyses the conversion of GMP to inosine 5′-monophosphate and contains a cystathionine-β-synthase (CBS) domain. Here the authors combine X-ray crystallography and kinetic measurements and present the GMP-, GTP-bound and nucleotide-fr...

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Autores principales:	Akira Imamura, Tetsuya Okada, Hikaru Mase, Takuya Otani, Tomoka Kobayashi, Manatsu Tamura, Bruno Kilunga Kubata, Katsuaki Inoue, Robert P. Rambo, Susumu Uchiyama, Kentaro Ishii, Shigenori Nishimura, Takashi Inui
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/285564165cb4499dbcb3b1543a57c253
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spelling	oai:doaj.org-article:285564165cb4499dbcb3b1543a57c2532021-12-02T15:39:24ZAllosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-β-synthase domain10.1038/s41467-020-15611-32041-1723https://doaj.org/article/285564165cb4499dbcb3b1543a57c2532020-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15611-3https://doaj.org/toc/2041-1723Trypanosoma brucei guanosine 5′-monophosphate reductase (TbGMPR) catalyses the conversion of GMP to inosine 5′-monophosphate and contains a cystathionine-β-synthase (CBS) domain. Here the authors combine X-ray crystallography and kinetic measurements and present the GMP-, GTP-bound and nucleotide-free TbGMPR structures and show that guanine and adenine nucleotides are allosteric regulators of TbGMPR that bind to the CBS domain.Akira ImamuraTetsuya OkadaHikaru MaseTakuya OtaniTomoka KobayashiManatsu TamuraBruno Kilunga KubataKatsuaki InoueRobert P. RamboSusumu UchiyamaKentaro IshiiShigenori NishimuraTakashi InuiNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Akira Imamura Tetsuya Okada Hikaru Mase Takuya Otani Tomoka Kobayashi Manatsu Tamura Bruno Kilunga Kubata Katsuaki Inoue Robert P. Rambo Susumu Uchiyama Kentaro Ishii Shigenori Nishimura Takashi Inui Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-β-synthase domain
description	Trypanosoma brucei guanosine 5′-monophosphate reductase (TbGMPR) catalyses the conversion of GMP to inosine 5′-monophosphate and contains a cystathionine-β-synthase (CBS) domain. Here the authors combine X-ray crystallography and kinetic measurements and present the GMP-, GTP-bound and nucleotide-free TbGMPR structures and show that guanine and adenine nucleotides are allosteric regulators of TbGMPR that bind to the CBS domain.
format	article
author	Akira Imamura Tetsuya Okada Hikaru Mase Takuya Otani Tomoka Kobayashi Manatsu Tamura Bruno Kilunga Kubata Katsuaki Inoue Robert P. Rambo Susumu Uchiyama Kentaro Ishii Shigenori Nishimura Takashi Inui
author_facet	Akira Imamura Tetsuya Okada Hikaru Mase Takuya Otani Tomoka Kobayashi Manatsu Tamura Bruno Kilunga Kubata Katsuaki Inoue Robert P. Rambo Susumu Uchiyama Kentaro Ishii Shigenori Nishimura Takashi Inui
author_sort	Akira Imamura
title	Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-β-synthase domain
title_short	Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-β-synthase domain
title_full	Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-β-synthase domain
title_fullStr	Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-β-synthase domain
title_full_unstemmed	Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-β-synthase domain
title_sort	allosteric regulation accompanied by oligomeric state changes of trypanosoma brucei gmp reductase through cystathionine-β-synthase domain
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/285564165cb4499dbcb3b1543a57c253
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Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-β-synthase domain

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