Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes

Abstract Mastitis is a common and costly disease on dairy farms, commonly caused by Staphylococcus spp. though the various species are associated with different clinical outcomes. In the current study, we performed genomic analyses to determine the prevalence of adhesion, biofilm, and related regula...

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Autores principales:	Lucas José Luduverio Pizauro, Camila Chioda de Almeida, Saura Rodrigues Silva, Janet I. MacInnes, Andrew M. Kropinski, Luiz Francisco Zafalon, Fernando Antônio de Avila, Alessandro de Mello Varani
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2861cccde8f844dd893abfaf7b26b5a1
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spelling	oai:doaj.org-article:2861cccde8f844dd893abfaf7b26b5a12021-12-02T19:09:20ZGenomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes10.1038/s41598-021-96842-22045-2322https://doaj.org/article/2861cccde8f844dd893abfaf7b26b5a12021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96842-2https://doaj.org/toc/2045-2322Abstract Mastitis is a common and costly disease on dairy farms, commonly caused by Staphylococcus spp. though the various species are associated with different clinical outcomes. In the current study, we performed genomic analyses to determine the prevalence of adhesion, biofilm, and related regulatory genes in 478 staphylococcal species isolated from clinical and subclinical mastitis cases deposited in public databases. The most prevalent adhesin genes (ebpS, atl, pls, sasH and sasF) were found in both clinical and subclinical isolates. However, the ebpS gene was absent in subclinical isolates of Staphylococcus arlettae, S. succinus, S. sciuri, S. equorun, S. galinarum, and S. saprophyticus. In contrast, the coa, eap, emp, efb, and vWbp genes were present more frequently in clinical (vs. subclincal) mastitis isolates and were highly correlated with the presence of the biofim operon (icaABCD) and its transcriptional regulator, icaR. Co-phylogenetic analyses suggested that many of these adhesins, biofilm, and associated regulatory genes could have been horizontally disseminated between clinical and subclinical isolates. Our results further suggest that several adhesins, biofilm, and related regulatory genes, which have been overlooked in previous studies, may be of use for virulence profiling of mastitis-related Staphylococcus strains or as potential targets for vaccine development.Lucas José Luduverio PizauroCamila Chioda de AlmeidaSaura Rodrigues SilvaJanet I. MacInnesAndrew M. KropinskiLuiz Francisco ZafalonFernando Antônio de AvilaAlessandro de Mello VaraniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lucas José Luduverio Pizauro Camila Chioda de Almeida Saura Rodrigues Silva Janet I. MacInnes Andrew M. Kropinski Luiz Francisco Zafalon Fernando Antônio de Avila Alessandro de Mello Varani Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes
description	Abstract Mastitis is a common and costly disease on dairy farms, commonly caused by Staphylococcus spp. though the various species are associated with different clinical outcomes. In the current study, we performed genomic analyses to determine the prevalence of adhesion, biofilm, and related regulatory genes in 478 staphylococcal species isolated from clinical and subclinical mastitis cases deposited in public databases. The most prevalent adhesin genes (ebpS, atl, pls, sasH and sasF) were found in both clinical and subclinical isolates. However, the ebpS gene was absent in subclinical isolates of Staphylococcus arlettae, S. succinus, S. sciuri, S. equorun, S. galinarum, and S. saprophyticus. In contrast, the coa, eap, emp, efb, and vWbp genes were present more frequently in clinical (vs. subclincal) mastitis isolates and were highly correlated with the presence of the biofim operon (icaABCD) and its transcriptional regulator, icaR. Co-phylogenetic analyses suggested that many of these adhesins, biofilm, and associated regulatory genes could have been horizontally disseminated between clinical and subclinical isolates. Our results further suggest that several adhesins, biofilm, and related regulatory genes, which have been overlooked in previous studies, may be of use for virulence profiling of mastitis-related Staphylococcus strains or as potential targets for vaccine development.
format	article
author	Lucas José Luduverio Pizauro Camila Chioda de Almeida Saura Rodrigues Silva Janet I. MacInnes Andrew M. Kropinski Luiz Francisco Zafalon Fernando Antônio de Avila Alessandro de Mello Varani
author_facet	Lucas José Luduverio Pizauro Camila Chioda de Almeida Saura Rodrigues Silva Janet I. MacInnes Andrew M. Kropinski Luiz Francisco Zafalon Fernando Antônio de Avila Alessandro de Mello Varani
author_sort	Lucas José Luduverio Pizauro
title	Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes
title_short	Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes
title_full	Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes
title_fullStr	Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes
title_full_unstemmed	Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes
title_sort	genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2861cccde8f844dd893abfaf7b26b5a1
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Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes

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