Preparation, characterization, and in vitro targeted delivery of folate-decorated paclitaxel-loaded bovine serum albumin nanoparticles

Dongmei Zhao, Xiuhua Zhao, Yuangang Zu, Jialei Li, Yu Zhang, Ru Jiang, Zhonghua ZhangKey Laboratory of Forest Plant Ecology, Northeast Forestry University, Ministry of Education, Harbin, Heilongjiang, ChinaAbstract: Paclitaxel (Taxol&reg;) is an important anticancer drug in clinical use for...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dongmei Zhao, Xiuhua Zhao, Yuangang Zu, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/286e24c875df401288721a73bc15d903
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:286e24c875df401288721a73bc15d903
record_format	dspace
spelling	oai:doaj.org-article:286e24c875df401288721a73bc15d9032021-12-02T05:02:17ZPreparation, characterization, and in vitro targeted delivery of folate-decorated paclitaxel-loaded bovine serum albumin nanoparticles1176-91141178-2013https://doaj.org/article/286e24c875df401288721a73bc15d9032010-09-01T00:00:00Zhttp://www.dovepress.com/preparation-characterization-and-in-vitro-targeted-delivery-of-folate--a5264https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Dongmei Zhao, Xiuhua Zhao, Yuangang Zu, Jialei Li, Yu Zhang, Ru Jiang, Zhonghua ZhangKey Laboratory of Forest Plant Ecology, Northeast Forestry University, Ministry of Education, Harbin, Heilongjiang, ChinaAbstract: Paclitaxel (Taxol&reg;) is an important anticancer drug in clinical use for treatment of a variety of cancers. Because of its low solubility, it is formulated in high concentration in Cremophor EL&reg; which induces hypersensitivity reactions. In this study, targeted delivery of paclitaxel-loaded nanoparticles was prepared by a desolvation procedure, crosslinked on the wall material of bovine serum albumin, and subsequently decorated by folic acid. The characteristics of the nanoparticles, such as amount of folate conjugation, surface morphology, drug entrapment efficiency, drug loading efficiency, and release kinetics were investigated in vitro. The targeting effect was investigated in vitro by cancer cell uptake of fluorescein isothiocyanate-labeled nanoparticles. The spherical nanoparticles obtained were negatively charged with a zeta potential of about -30 mV, and characterized around 210 nm with a narrow size distribution. Drug entrapment efficiency and drug loading efficiency were approximately 95.3% and 27.2%, respectively. The amount of folate conjugation was 9.22 &micro;g/mg of bovine serum albumin. The folate-decorated nanoparticles targeted a human prostate cancer cell line effectively.Keywords: paclitaxel, bovine serum albumin, folate, nanoparticles, target delivery Dongmei ZhaoXiuhua ZhaoYuangang Zuet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 669-677 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Dongmei Zhao Xiuhua Zhao Yuangang Zu et al Preparation, characterization, and in vitro targeted delivery of folate-decorated paclitaxel-loaded bovine serum albumin nanoparticles
description	Dongmei Zhao, Xiuhua Zhao, Yuangang Zu, Jialei Li, Yu Zhang, Ru Jiang, Zhonghua ZhangKey Laboratory of Forest Plant Ecology, Northeast Forestry University, Ministry of Education, Harbin, Heilongjiang, ChinaAbstract: Paclitaxel (Taxol&reg;) is an important anticancer drug in clinical use for treatment of a variety of cancers. Because of its low solubility, it is formulated in high concentration in Cremophor EL&reg; which induces hypersensitivity reactions. In this study, targeted delivery of paclitaxel-loaded nanoparticles was prepared by a desolvation procedure, crosslinked on the wall material of bovine serum albumin, and subsequently decorated by folic acid. The characteristics of the nanoparticles, such as amount of folate conjugation, surface morphology, drug entrapment efficiency, drug loading efficiency, and release kinetics were investigated in vitro. The targeting effect was investigated in vitro by cancer cell uptake of fluorescein isothiocyanate-labeled nanoparticles. The spherical nanoparticles obtained were negatively charged with a zeta potential of about -30 mV, and characterized around 210 nm with a narrow size distribution. Drug entrapment efficiency and drug loading efficiency were approximately 95.3% and 27.2%, respectively. The amount of folate conjugation was 9.22 &micro;g/mg of bovine serum albumin. The folate-decorated nanoparticles targeted a human prostate cancer cell line effectively.Keywords: paclitaxel, bovine serum albumin, folate, nanoparticles, target delivery
format	article
author	Dongmei Zhao Xiuhua Zhao Yuangang Zu et al
author_facet	Dongmei Zhao Xiuhua Zhao Yuangang Zu et al
author_sort	Dongmei Zhao
title	Preparation, characterization, and in vitro targeted delivery of folate-decorated paclitaxel-loaded bovine serum albumin nanoparticles
title_short	Preparation, characterization, and in vitro targeted delivery of folate-decorated paclitaxel-loaded bovine serum albumin nanoparticles
title_full	Preparation, characterization, and in vitro targeted delivery of folate-decorated paclitaxel-loaded bovine serum albumin nanoparticles
title_fullStr	Preparation, characterization, and in vitro targeted delivery of folate-decorated paclitaxel-loaded bovine serum albumin nanoparticles
title_full_unstemmed	Preparation, characterization, and in vitro targeted delivery of folate-decorated paclitaxel-loaded bovine serum albumin nanoparticles
title_sort	preparation, characterization, and in vitro targeted delivery of folate-decorated paclitaxel-loaded bovine serum albumin nanoparticles
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/286e24c875df401288721a73bc15d903
work_keys_str_mv	AT dongmeizhao preparationcharacterizationandinvitrotargeteddeliveryoffolatedecoratedpaclitaxelloadedbovineserumalbuminnanoparticles AT xiuhuazhao preparationcharacterizationandinvitrotargeteddeliveryoffolatedecoratedpaclitaxelloadedbovineserumalbuminnanoparticles AT yuangangzu preparationcharacterizationandinvitrotargeteddeliveryoffolatedecoratedpaclitaxelloadedbovineserumalbuminnanoparticles AT etal preparationcharacterizationandinvitrotargeteddeliveryoffolatedecoratedpaclitaxelloadedbovineserumalbuminnanoparticles
_version_	1718400758874898432

Preparation, characterization, and in vitro targeted delivery of folate-decorated paclitaxel-loaded bovine serum albumin nanoparticles

Ejemplares similares