Design of a multi-epitope vaccine against cervical cancer using immunoinformatics approaches

Abstract Cervical cancer, caused by human papillomavirus (HPV), is the fourth most common type of cancer among women worldwide. While HPV prophylactic vaccines are available, they have no therapeutic effects and do not clear up existing infections. This study aims to design a therapeutic vaccine aga...

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Autores principales:	Samira Sanami, Fatemeh Azadegan-Dehkordi, Mahmoud Rafieian-Kopaei, Majid Salehi, Maryam Ghasemi-Dehnoo, Mehran Mahooti, Morteza Alizadeh, Nader Bagheri
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2872f49638ee41a9b6d96c22f9350fa5
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spelling	oai:doaj.org-article:2872f49638ee41a9b6d96c22f9350fa52021-12-02T17:47:15ZDesign of a multi-epitope vaccine against cervical cancer using immunoinformatics approaches10.1038/s41598-021-91997-42045-2322https://doaj.org/article/2872f49638ee41a9b6d96c22f9350fa52021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91997-4https://doaj.org/toc/2045-2322Abstract Cervical cancer, caused by human papillomavirus (HPV), is the fourth most common type of cancer among women worldwide. While HPV prophylactic vaccines are available, they have no therapeutic effects and do not clear up existing infections. This study aims to design a therapeutic vaccine against cervical cancer using reverse vaccinology. In this study, the E6 and E7 oncoproteins from HPV16 were chosen as the target antigens for epitope prediction. Cytotoxic T lymphocytes (CTL) and helper T lymphocytes (HTL) epitopes were predicted, and the best epitopes were selected based on antigenicity, allergenicity, and toxicity. The final vaccine construct was composed of the selected epitopes, along with the appropriate adjuvant and linkers. The multi-epitope vaccine was evaluated in terms of physicochemical properties, antigenicity, and allergenicity. The tertiary structure of the vaccine construct was predicted. Furthermore, several analyses were also carried out, including molecular docking, molecular dynamics (MD) simulation, and in silico cloning of the vaccine construct. The results showed that the final proposed vaccine could be considered an effective therapeutic vaccine for HPV; however, in vitro and in vivo experiments are required to validate the efficacy of this vaccine candidate.Samira SanamiFatemeh Azadegan-DehkordiMahmoud Rafieian-KopaeiMajid SalehiMaryam Ghasemi-DehnooMehran MahootiMorteza AlizadehNader BagheriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Samira Sanami Fatemeh Azadegan-Dehkordi Mahmoud Rafieian-Kopaei Majid Salehi Maryam Ghasemi-Dehnoo Mehran Mahooti Morteza Alizadeh Nader Bagheri Design of a multi-epitope vaccine against cervical cancer using immunoinformatics approaches
description	Abstract Cervical cancer, caused by human papillomavirus (HPV), is the fourth most common type of cancer among women worldwide. While HPV prophylactic vaccines are available, they have no therapeutic effects and do not clear up existing infections. This study aims to design a therapeutic vaccine against cervical cancer using reverse vaccinology. In this study, the E6 and E7 oncoproteins from HPV16 were chosen as the target antigens for epitope prediction. Cytotoxic T lymphocytes (CTL) and helper T lymphocytes (HTL) epitopes were predicted, and the best epitopes were selected based on antigenicity, allergenicity, and toxicity. The final vaccine construct was composed of the selected epitopes, along with the appropriate adjuvant and linkers. The multi-epitope vaccine was evaluated in terms of physicochemical properties, antigenicity, and allergenicity. The tertiary structure of the vaccine construct was predicted. Furthermore, several analyses were also carried out, including molecular docking, molecular dynamics (MD) simulation, and in silico cloning of the vaccine construct. The results showed that the final proposed vaccine could be considered an effective therapeutic vaccine for HPV; however, in vitro and in vivo experiments are required to validate the efficacy of this vaccine candidate.
format	article
author	Samira Sanami Fatemeh Azadegan-Dehkordi Mahmoud Rafieian-Kopaei Majid Salehi Maryam Ghasemi-Dehnoo Mehran Mahooti Morteza Alizadeh Nader Bagheri
author_facet	Samira Sanami Fatemeh Azadegan-Dehkordi Mahmoud Rafieian-Kopaei Majid Salehi Maryam Ghasemi-Dehnoo Mehran Mahooti Morteza Alizadeh Nader Bagheri
author_sort	Samira Sanami
title	Design of a multi-epitope vaccine against cervical cancer using immunoinformatics approaches
title_short	Design of a multi-epitope vaccine against cervical cancer using immunoinformatics approaches
title_full	Design of a multi-epitope vaccine against cervical cancer using immunoinformatics approaches
title_fullStr	Design of a multi-epitope vaccine against cervical cancer using immunoinformatics approaches
title_full_unstemmed	Design of a multi-epitope vaccine against cervical cancer using immunoinformatics approaches
title_sort	design of a multi-epitope vaccine against cervical cancer using immunoinformatics approaches
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2872f49638ee41a9b6d96c22f9350fa5
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Design of a multi-epitope vaccine against cervical cancer using immunoinformatics approaches

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