Increased Frequency of CTLA-4 and PD-1 Expressing Regulatory T Cells and Basophils With an Activating Profile in Infants With Moderate-to-Severe Atopic Dermatitis Hypersensitized to Food Allergens

Background: Infants with severe atopic dermatitis (AD) may be sensitized to foods that have not been introduced into their diet, posing a risk for developing an immediate hypersensitivity reaction on the first exposure to the food to which they are sensitized. The aim of this work was to perform an...

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Autores principales:	Agurtzane Bilbao, Raquel Pérez-Garay, Idoia Rius, Alex Irurzun, Iñigo Terrén, Ane Orrantia, Gabirel Astarloa-Pando, Francisco Borrego, Olatz Zenarruzabeitia
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	atopic dermatitis food sensitization specific IgE regulatory T cells basophils CD300 receptors Pediatrics RJ1-570
Acceso en línea:	https://doaj.org/article/2876b71c30334d21a42ab173441b6175
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spelling	oai:doaj.org-article:2876b71c30334d21a42ab173441b61752021-12-01T13:53:27ZIncreased Frequency of CTLA-4 and PD-1 Expressing Regulatory T Cells and Basophils With an Activating Profile in Infants With Moderate-to-Severe Atopic Dermatitis Hypersensitized to Food Allergens2296-236010.3389/fped.2021.734645https://doaj.org/article/2876b71c30334d21a42ab173441b61752021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fped.2021.734645/fullhttps://doaj.org/toc/2296-2360Background: Infants with severe atopic dermatitis (AD) may be sensitized to foods that have not been introduced into their diet, posing a risk for developing an immediate hypersensitivity reaction on the first exposure to the food to which they are sensitized. The aim of this work was to perform an analysis of the sensitization profile in infants with moderate-to-severe AD and to identify cellular and molecular markers for food allergy (FA).Methods: Blood samples from healthy donors and children with moderate-to-severe AD were studied. Specific IgE to several allergens were determined using ImmunoCAP FEIA system and ISAC technology. Furthermore, using flow cytometry-based studies, basophils and regulatory T (Treg) cells were phenotypically characterized.Results: 90% of children with AD were sensitized to food antigens before introducing them into the diet, and 100% developed FA. Phenotypic analysis showed a significantly higher percentage of CTLA-4 and PD-1 expressing Treg cells in AD patients than in healthy controls. Basophils from patients exhibited a marked reduction in the expression of CD300a, higher expression of FcεRI and CXCR4, and to some extent higher expression of CD63 and CD300c.Conclusions: Infants with moderate-to-severe AD are at high risk of being sensitized to food allergens. Therefore, to avoid allergic reactions, broad-spectrum sensitization studies are necessary before introducing complementary diet. Increased expression of CTLA-4 and PD-1 suggests greater suppressive potential of Treg cells in infants with AD than healthy controls. Furthermore, our results suggest a role for CD300 molecules on circulating basophils as possible biomarkers for FA susceptibility.Agurtzane BilbaoAgurtzane BilbaoRaquel Pérez-GarayRaquel Pérez-GarayIdoia RiusAlex IrurzunIñigo TerrénAne OrrantiaGabirel Astarloa-PandoFrancisco BorregoFrancisco BorregoOlatz ZenarruzabeitiaFrontiers Media S.A.articleatopic dermatitisfood sensitizationspecific IgEregulatory T cellsbasophilsCD300 receptorsPediatricsRJ1-570ENFrontiers in Pediatrics, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	atopic dermatitis food sensitization specific IgE regulatory T cells basophils CD300 receptors Pediatrics RJ1-570
spellingShingle	atopic dermatitis food sensitization specific IgE regulatory T cells basophils CD300 receptors Pediatrics RJ1-570 Agurtzane Bilbao Agurtzane Bilbao Raquel Pérez-Garay Raquel Pérez-Garay Idoia Rius Alex Irurzun Iñigo Terrén Ane Orrantia Gabirel Astarloa-Pando Francisco Borrego Francisco Borrego Olatz Zenarruzabeitia Increased Frequency of CTLA-4 and PD-1 Expressing Regulatory T Cells and Basophils With an Activating Profile in Infants With Moderate-to-Severe Atopic Dermatitis Hypersensitized to Food Allergens
description	Background: Infants with severe atopic dermatitis (AD) may be sensitized to foods that have not been introduced into their diet, posing a risk for developing an immediate hypersensitivity reaction on the first exposure to the food to which they are sensitized. The aim of this work was to perform an analysis of the sensitization profile in infants with moderate-to-severe AD and to identify cellular and molecular markers for food allergy (FA).Methods: Blood samples from healthy donors and children with moderate-to-severe AD were studied. Specific IgE to several allergens were determined using ImmunoCAP FEIA system and ISAC technology. Furthermore, using flow cytometry-based studies, basophils and regulatory T (Treg) cells were phenotypically characterized.Results: 90% of children with AD were sensitized to food antigens before introducing them into the diet, and 100% developed FA. Phenotypic analysis showed a significantly higher percentage of CTLA-4 and PD-1 expressing Treg cells in AD patients than in healthy controls. Basophils from patients exhibited a marked reduction in the expression of CD300a, higher expression of FcεRI and CXCR4, and to some extent higher expression of CD63 and CD300c.Conclusions: Infants with moderate-to-severe AD are at high risk of being sensitized to food allergens. Therefore, to avoid allergic reactions, broad-spectrum sensitization studies are necessary before introducing complementary diet. Increased expression of CTLA-4 and PD-1 suggests greater suppressive potential of Treg cells in infants with AD than healthy controls. Furthermore, our results suggest a role for CD300 molecules on circulating basophils as possible biomarkers for FA susceptibility.
format	article
author	Agurtzane Bilbao Agurtzane Bilbao Raquel Pérez-Garay Raquel Pérez-Garay Idoia Rius Alex Irurzun Iñigo Terrén Ane Orrantia Gabirel Astarloa-Pando Francisco Borrego Francisco Borrego Olatz Zenarruzabeitia
author_facet	Agurtzane Bilbao Agurtzane Bilbao Raquel Pérez-Garay Raquel Pérez-Garay Idoia Rius Alex Irurzun Iñigo Terrén Ane Orrantia Gabirel Astarloa-Pando Francisco Borrego Francisco Borrego Olatz Zenarruzabeitia
author_sort	Agurtzane Bilbao
title	Increased Frequency of CTLA-4 and PD-1 Expressing Regulatory T Cells and Basophils With an Activating Profile in Infants With Moderate-to-Severe Atopic Dermatitis Hypersensitized to Food Allergens
title_short	Increased Frequency of CTLA-4 and PD-1 Expressing Regulatory T Cells and Basophils With an Activating Profile in Infants With Moderate-to-Severe Atopic Dermatitis Hypersensitized to Food Allergens
title_full	Increased Frequency of CTLA-4 and PD-1 Expressing Regulatory T Cells and Basophils With an Activating Profile in Infants With Moderate-to-Severe Atopic Dermatitis Hypersensitized to Food Allergens
title_fullStr	Increased Frequency of CTLA-4 and PD-1 Expressing Regulatory T Cells and Basophils With an Activating Profile in Infants With Moderate-to-Severe Atopic Dermatitis Hypersensitized to Food Allergens
title_full_unstemmed	Increased Frequency of CTLA-4 and PD-1 Expressing Regulatory T Cells and Basophils With an Activating Profile in Infants With Moderate-to-Severe Atopic Dermatitis Hypersensitized to Food Allergens
title_sort	increased frequency of ctla-4 and pd-1 expressing regulatory t cells and basophils with an activating profile in infants with moderate-to-severe atopic dermatitis hypersensitized to food allergens
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2876b71c30334d21a42ab173441b6175
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Increased Frequency of CTLA-4 and PD-1 Expressing Regulatory T Cells and Basophils With an Activating Profile in Infants With Moderate-to-Severe Atopic Dermatitis Hypersensitized to Food Allergens

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