Quantitative X-ray tomographic analysis reveals calcium precipitation in cataractogenesis

Abstract Cataracts, named for pathological light scattering in the lens, are known to be associated with increased large protein aggregates, disrupted protein phase separation, and/or osmotic imbalances in lens cells. We have applied synchrotron phase contrast X-ray micro-computed tomography to dire...

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Autores principales:	Yuxing Li, Dilworth Y. Parkinson, Jun Feng, Chun-hong Xia, Xiaohua Gong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/28788108e6464434a3ef5b28e8b7c220
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spelling	oai:doaj.org-article:28788108e6464434a3ef5b28e8b7c2202021-12-02T19:10:21ZQuantitative X-ray tomographic analysis reveals calcium precipitation in cataractogenesis10.1038/s41598-021-96867-72045-2322https://doaj.org/article/28788108e6464434a3ef5b28e8b7c2202021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96867-7https://doaj.org/toc/2045-2322Abstract Cataracts, named for pathological light scattering in the lens, are known to be associated with increased large protein aggregates, disrupted protein phase separation, and/or osmotic imbalances in lens cells. We have applied synchrotron phase contrast X-ray micro-computed tomography to directly examine an age-related nuclear cataract model in Cx46 knockout (Cx46KO) mice. High-resolution 3D X-ray tomographic images reveal amorphous spots and strip-like dense matter precipitates in lens cores of all examined Cx46KO mice at different ages. The precipitates are predominantly accumulated in the anterior suture regions of lens cores, and they become longer and dense as mice age. Alizarin red staining data confirms the presence of calcium precipitates in lens cores of all Cx46KO mice. This study indicates that the spatial and temporal calcium precipitation is an age-related event associated with age-related nuclear cataract formation in Cx46KO mice, and further suggests that the loss of Cx46 promotes calcium precipitates in the lens core, which is a new mechanism that likely contributes to the pathological light scattering in this age-related cataract model.Yuxing LiDilworth Y. ParkinsonJun FengChun-hong XiaXiaohua GongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuxing Li Dilworth Y. Parkinson Jun Feng Chun-hong Xia Xiaohua Gong Quantitative X-ray tomographic analysis reveals calcium precipitation in cataractogenesis
description	Abstract Cataracts, named for pathological light scattering in the lens, are known to be associated with increased large protein aggregates, disrupted protein phase separation, and/or osmotic imbalances in lens cells. We have applied synchrotron phase contrast X-ray micro-computed tomography to directly examine an age-related nuclear cataract model in Cx46 knockout (Cx46KO) mice. High-resolution 3D X-ray tomographic images reveal amorphous spots and strip-like dense matter precipitates in lens cores of all examined Cx46KO mice at different ages. The precipitates are predominantly accumulated in the anterior suture regions of lens cores, and they become longer and dense as mice age. Alizarin red staining data confirms the presence of calcium precipitates in lens cores of all Cx46KO mice. This study indicates that the spatial and temporal calcium precipitation is an age-related event associated with age-related nuclear cataract formation in Cx46KO mice, and further suggests that the loss of Cx46 promotes calcium precipitates in the lens core, which is a new mechanism that likely contributes to the pathological light scattering in this age-related cataract model.
format	article
author	Yuxing Li Dilworth Y. Parkinson Jun Feng Chun-hong Xia Xiaohua Gong
author_facet	Yuxing Li Dilworth Y. Parkinson Jun Feng Chun-hong Xia Xiaohua Gong
author_sort	Yuxing Li
title	Quantitative X-ray tomographic analysis reveals calcium precipitation in cataractogenesis
title_short	Quantitative X-ray tomographic analysis reveals calcium precipitation in cataractogenesis
title_full	Quantitative X-ray tomographic analysis reveals calcium precipitation in cataractogenesis
title_fullStr	Quantitative X-ray tomographic analysis reveals calcium precipitation in cataractogenesis
title_full_unstemmed	Quantitative X-ray tomographic analysis reveals calcium precipitation in cataractogenesis
title_sort	quantitative x-ray tomographic analysis reveals calcium precipitation in cataractogenesis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/28788108e6464434a3ef5b28e8b7c220
work_keys_str_mv	AT yuxingli quantitativexraytomographicanalysisrevealscalciumprecipitationincataractogenesis AT dilworthyparkinson quantitativexraytomographicanalysisrevealscalciumprecipitationincataractogenesis AT junfeng quantitativexraytomographicanalysisrevealscalciumprecipitationincataractogenesis AT chunhongxia quantitativexraytomographicanalysisrevealscalciumprecipitationincataractogenesis AT xiaohuagong quantitativexraytomographicanalysisrevealscalciumprecipitationincataractogenesis
_version_	1718377078471000064

Quantitative X-ray tomographic analysis reveals calcium precipitation in cataractogenesis

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