A multidimensional phasor approach reveals LAURDAN photophysics in NIH-3T3 cell membranes

Abstract Mammalian cell membranes have different phospholipid composition and cholesterol content, displaying a profile of fluidity that depends on their intracellular location. Among the dyes used in membrane studies, LAURDAN has the advantage to be sensitive to the lipid composition as well as to...

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Autores principales:	Leonel Malacrida, David M. Jameson, Enrico Gratton
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/28801a7bcf3d47ab8ff403554ee8bd79
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spelling	oai:doaj.org-article:28801a7bcf3d47ab8ff403554ee8bd792021-12-02T12:32:16ZA multidimensional phasor approach reveals LAURDAN photophysics in NIH-3T3 cell membranes10.1038/s41598-017-08564-z2045-2322https://doaj.org/article/28801a7bcf3d47ab8ff403554ee8bd792017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08564-zhttps://doaj.org/toc/2045-2322Abstract Mammalian cell membranes have different phospholipid composition and cholesterol content, displaying a profile of fluidity that depends on their intracellular location. Among the dyes used in membrane studies, LAURDAN has the advantage to be sensitive to the lipid composition as well as to membrane fluidity. The LAURDAN spectrum is sensitive to the lipid composition and dipolar relaxation arising from water penetration, but disentangling lipid composition from membrane fluidity can be obtained if time resolved spectra could be measured at each cell location. Here we describe a method in which spectral and lifetime information obtained in different measurements at the same plane in a cell are used in the phasor plot providing a solution to analyze multiple lifetime or spectral data through a common visualization approach. We exploit a property of phasor plots based on the reciprocal role of the phasor plot and the image. In the phasor analysis each pixel of the image is associated with a phasor and each phasor maps to pixels and features in the image. In this paper the lifetime and spectral fluorescence data are used simultaneously to determine the contribution of polarity and dipolar relaxations of LAURDAN in each pixel of an image.Leonel MalacridaDavid M. JamesonEnrico GrattonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Leonel Malacrida David M. Jameson Enrico Gratton A multidimensional phasor approach reveals LAURDAN photophysics in NIH-3T3 cell membranes
description	Abstract Mammalian cell membranes have different phospholipid composition and cholesterol content, displaying a profile of fluidity that depends on their intracellular location. Among the dyes used in membrane studies, LAURDAN has the advantage to be sensitive to the lipid composition as well as to membrane fluidity. The LAURDAN spectrum is sensitive to the lipid composition and dipolar relaxation arising from water penetration, but disentangling lipid composition from membrane fluidity can be obtained if time resolved spectra could be measured at each cell location. Here we describe a method in which spectral and lifetime information obtained in different measurements at the same plane in a cell are used in the phasor plot providing a solution to analyze multiple lifetime or spectral data through a common visualization approach. We exploit a property of phasor plots based on the reciprocal role of the phasor plot and the image. In the phasor analysis each pixel of the image is associated with a phasor and each phasor maps to pixels and features in the image. In this paper the lifetime and spectral fluorescence data are used simultaneously to determine the contribution of polarity and dipolar relaxations of LAURDAN in each pixel of an image.
format	article
author	Leonel Malacrida David M. Jameson Enrico Gratton
author_facet	Leonel Malacrida David M. Jameson Enrico Gratton
author_sort	Leonel Malacrida
title	A multidimensional phasor approach reveals LAURDAN photophysics in NIH-3T3 cell membranes
title_short	A multidimensional phasor approach reveals LAURDAN photophysics in NIH-3T3 cell membranes
title_full	A multidimensional phasor approach reveals LAURDAN photophysics in NIH-3T3 cell membranes
title_fullStr	A multidimensional phasor approach reveals LAURDAN photophysics in NIH-3T3 cell membranes
title_full_unstemmed	A multidimensional phasor approach reveals LAURDAN photophysics in NIH-3T3 cell membranes
title_sort	multidimensional phasor approach reveals laurdan photophysics in nih-3t3 cell membranes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/28801a7bcf3d47ab8ff403554ee8bd79
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A multidimensional phasor approach reveals LAURDAN photophysics in NIH-3T3 cell membranes

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