Stimulation of IgM Production in HB4C5 Cell Line and Mouse Splenocytes by Egg Yolk Extract from the Egg of Indonesian Native Chicken

Kampung-chicken, as a local wisdom, has always been considered superior in terms of health benefits compared to commercial chicken. Therefore, this study was designed to evaluate the immunostimulatory effects of Kampung-chicken eggs using in vitro approaches. The physical (interior and exterior qua...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	R. Mulyani, E. Harmayani, Nurliyani, K. Nishi, M. Ishida, T. Sugahara
Formato:	article
Lenguaje:	EN
Publicado:	IPB University 2021
Materias:	native-chicken egg yolk immunostimulatory activity HB4C5 cells immunoglobulin production mouse primary splenocytes Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/2880591797c24e2a9dcf03f49714f9ed
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2880591797c24e2a9dcf03f49714f9ed
record_format	dspace
spelling	oai:doaj.org-article:2880591797c24e2a9dcf03f49714f9ed2021-11-15T23:17:07ZStimulation of IgM Production in HB4C5 Cell Line and Mouse Splenocytes by Egg Yolk Extract from the Egg of Indonesian Native Chicken10.5398/tasj.2021.44.4.5112615-787X2615-790Xhttps://doaj.org/article/2880591797c24e2a9dcf03f49714f9ed2021-11-01T00:00:00Zhttps://journal.ipb.ac.id/index.php/tasj/article/view/33641https://doaj.org/toc/2615-787Xhttps://doaj.org/toc/2615-790X Kampung-chicken, as a local wisdom, has always been considered superior in terms of health benefits compared to commercial chicken. Therefore, this study was designed to evaluate the immunostimulatory effects of Kampung-chicken eggs using in vitro approaches. The physical (interior and exterior quality) and chemical characteristics of Kampung-chicken eggs were firstly analyzed; the commercial chicken egg was used as a comparison. The effects of water extract of Kampung-chicken egg yolk (KEYWE) and water extract of commercial-chicken egg yolk (CEYWE) on IgM production and the expression of IgM-gene on HB4C5 cells were analyzed. In addition, the stimulatory effects of both samples on IgA, IgG, and IgM production by mouse primary splenocytes were analyzed as well. To isolate the active substance, the water extract from egg yolk was partially purified by salting out using (NH4)2SO4. Data analysis of the comparison between Kampung-chicken egg and the commercial-chicken egg was performed by t-test; meanwhile, the results for in vitro studies were performed by one-way ANOVA, and Tukey’s multiple comparison test was used for posthoc analysis. The result showed that KEYWE increased IgM production in HB4C5 cells by 5.91 fold as compared with control. In addition, KEYWE increased IgA, IgG, and IgM productions in mouse primary splenocytes by 5.20, 2.54, and 3.1 fold, respectively. Moreover, the immunoglobulin (Ig) production-stimulating activities of KEYWE on HB4C5 cells and mouse primary splenocytes were higher than those of CEYWE. The active substance in Kampung-chicken egg yolk was precipitated at 80% saturation of (NH4)2SO4. Altogether, it is expected that the Kampung-chicken egg has more effective immunostimulatory activity than the commercial chicken egg. R. MulyaniE. HarmayaniNurliyaniK. NishiM. IshidaT. SugaharaIPB Universityarticlenative-chicken egg yolkimmunostimulatory activityHB4C5 cellsimmunoglobulin productionmouse primary splenocytesAnimal cultureSF1-1100ENTropical Animal Science Journal, Vol 44, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	native-chicken egg yolk immunostimulatory activity HB4C5 cells immunoglobulin production mouse primary splenocytes Animal culture SF1-1100
spellingShingle	native-chicken egg yolk immunostimulatory activity HB4C5 cells immunoglobulin production mouse primary splenocytes Animal culture SF1-1100 R. Mulyani E. Harmayani Nurliyani K. Nishi M. Ishida T. Sugahara Stimulation of IgM Production in HB4C5 Cell Line and Mouse Splenocytes by Egg Yolk Extract from the Egg of Indonesian Native Chicken
description	Kampung-chicken, as a local wisdom, has always been considered superior in terms of health benefits compared to commercial chicken. Therefore, this study was designed to evaluate the immunostimulatory effects of Kampung-chicken eggs using in vitro approaches. The physical (interior and exterior quality) and chemical characteristics of Kampung-chicken eggs were firstly analyzed; the commercial chicken egg was used as a comparison. The effects of water extract of Kampung-chicken egg yolk (KEYWE) and water extract of commercial-chicken egg yolk (CEYWE) on IgM production and the expression of IgM-gene on HB4C5 cells were analyzed. In addition, the stimulatory effects of both samples on IgA, IgG, and IgM production by mouse primary splenocytes were analyzed as well. To isolate the active substance, the water extract from egg yolk was partially purified by salting out using (NH4)2SO4. Data analysis of the comparison between Kampung-chicken egg and the commercial-chicken egg was performed by t-test; meanwhile, the results for in vitro studies were performed by one-way ANOVA, and Tukey’s multiple comparison test was used for posthoc analysis. The result showed that KEYWE increased IgM production in HB4C5 cells by 5.91 fold as compared with control. In addition, KEYWE increased IgA, IgG, and IgM productions in mouse primary splenocytes by 5.20, 2.54, and 3.1 fold, respectively. Moreover, the immunoglobulin (Ig) production-stimulating activities of KEYWE on HB4C5 cells and mouse primary splenocytes were higher than those of CEYWE. The active substance in Kampung-chicken egg yolk was precipitated at 80% saturation of (NH4)2SO4. Altogether, it is expected that the Kampung-chicken egg has more effective immunostimulatory activity than the commercial chicken egg.
format	article
author	R. Mulyani E. Harmayani Nurliyani K. Nishi M. Ishida T. Sugahara
author_facet	R. Mulyani E. Harmayani Nurliyani K. Nishi M. Ishida T. Sugahara
author_sort	R. Mulyani
title	Stimulation of IgM Production in HB4C5 Cell Line and Mouse Splenocytes by Egg Yolk Extract from the Egg of Indonesian Native Chicken
title_short	Stimulation of IgM Production in HB4C5 Cell Line and Mouse Splenocytes by Egg Yolk Extract from the Egg of Indonesian Native Chicken
title_full	Stimulation of IgM Production in HB4C5 Cell Line and Mouse Splenocytes by Egg Yolk Extract from the Egg of Indonesian Native Chicken
title_fullStr	Stimulation of IgM Production in HB4C5 Cell Line and Mouse Splenocytes by Egg Yolk Extract from the Egg of Indonesian Native Chicken
title_full_unstemmed	Stimulation of IgM Production in HB4C5 Cell Line and Mouse Splenocytes by Egg Yolk Extract from the Egg of Indonesian Native Chicken
title_sort	stimulation of igm production in hb4c5 cell line and mouse splenocytes by egg yolk extract from the egg of indonesian native chicken
publisher	IPB University
publishDate	2021
url	https://doaj.org/article/2880591797c24e2a9dcf03f49714f9ed
work_keys_str_mv	AT rmulyani stimulationofigmproductioninhb4c5celllineandmousesplenocytesbyeggyolkextractfromtheeggofindonesiannativechicken AT eharmayani stimulationofigmproductioninhb4c5celllineandmousesplenocytesbyeggyolkextractfromtheeggofindonesiannativechicken AT nurliyani stimulationofigmproductioninhb4c5celllineandmousesplenocytesbyeggyolkextractfromtheeggofindonesiannativechicken AT knishi stimulationofigmproductioninhb4c5celllineandmousesplenocytesbyeggyolkextractfromtheeggofindonesiannativechicken AT mishida stimulationofigmproductioninhb4c5celllineandmousesplenocytesbyeggyolkextractfromtheeggofindonesiannativechicken AT tsugahara stimulationofigmproductioninhb4c5celllineandmousesplenocytesbyeggyolkextractfromtheeggofindonesiannativechicken
_version_	1718426772149633024

Stimulation of IgM Production in HB4C5 Cell Line and Mouse Splenocytes by Egg Yolk Extract from the Egg of Indonesian Native Chicken

Ejemplares similares