Short Communication: Preliminary phylogenetic analysis of bacteria producing laccase isolated from Gunung Pancar, Bogor, Indonesia

Abstract. Mar WW, Rohman A, Muwafiqi NH, Laras GA, Agustina D, Asmarani O, Puspaningsih NNT. 2020. Short Communication: Preliminary phylogenetic analysis of bacteria producing laccase isolated from Gunung Pancar, Bogor, Indonesia. Biodiversitas 21: 2113-2118. Interpretation of phylogenetic trees is...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	WIN WIN MAR, ALI ROHMAN, NUR H. MUWAFIQI, GALIH AYHUSTA LARAS, DYAH AGUSTINA, ONE ASMARANI, NI NYOMAN TRI PUSPANINGSIH
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	geobacillus kaustophilus tp-02, laccase, neighbor-joining method, phylogenetic tree Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2880e952bcb74c31adb0306c6a773ac4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2880e952bcb74c31adb0306c6a773ac4
record_format	dspace
spelling	oai:doaj.org-article:2880e952bcb74c31adb0306c6a773ac42021-11-22T00:35:33ZShort Communication: Preliminary phylogenetic analysis of bacteria producing laccase isolated from Gunung Pancar, Bogor, Indonesia1412-033X2085-472210.13057/biodiv/d210539https://doaj.org/article/2880e952bcb74c31adb0306c6a773ac42020-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5184https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Mar WW, Rohman A, Muwafiqi NH, Laras GA, Agustina D, Asmarani O, Puspaningsih NNT. 2020. Short Communication: Preliminary phylogenetic analysis of bacteria producing laccase isolated from Gunung Pancar, Bogor, Indonesia. Biodiversitas 21: 2113-2118. Interpretation of phylogenetic trees is essential in understanding relationships between organisms, as well as their characteristics, bionetwork, and even their genomic and developmental ecology. The aim of this research is to analyze the phylogenetic bacteria producing laccase isolated from Gunung Pancar Bogor, Indonesia. Phylogenetic analysis of Geobacillus kaustophilus TP-02 producing laccase was performed using alignments with all Bacillus clusters. A phylogenetic tree was constructed by the neighbor-joining (NJ) method, using the maximum likelihood parameter. Laccase was purified using ammonium sulfate precipitation to 2.67-fold. The activity of crude laccase was determined to be 93.39 U/ml using 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as the substrate. Moreover, Geobacillus related to counting some Geobacillus genus such as Geobacillus thermocatenulatus, Geobacillus sp. MAS1, Geobacillus thermoleovorans, and Geobacillus zalihae, having similarity under 91% with comparison to the Geobacillus kaustophilius sequence in GenBank. Therefore, results show that G. kaustophilus TP-02 does not have any closeness with other Geobacillus genera, even though these species have the same ancestor.WIN WIN MARALI ROHMANNUR H. MUWAFIQIGALIH AYHUSTA LARASDYAH AGUSTINAONE ASMARANINI NYOMAN TRI PUSPANINGSIHMBI & UNS Soloarticlegeobacillus kaustophilus tp-02, laccase, neighbor-joining method, phylogenetic treeBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 5 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	geobacillus kaustophilus tp-02, laccase, neighbor-joining method, phylogenetic tree Biology (General) QH301-705.5
spellingShingle	geobacillus kaustophilus tp-02, laccase, neighbor-joining method, phylogenetic tree Biology (General) QH301-705.5 WIN WIN MAR ALI ROHMAN NUR H. MUWAFIQI GALIH AYHUSTA LARAS DYAH AGUSTINA ONE ASMARANI NI NYOMAN TRI PUSPANINGSIH Short Communication: Preliminary phylogenetic analysis of bacteria producing laccase isolated from Gunung Pancar, Bogor, Indonesia
description	Abstract. Mar WW, Rohman A, Muwafiqi NH, Laras GA, Agustina D, Asmarani O, Puspaningsih NNT. 2020. Short Communication: Preliminary phylogenetic analysis of bacteria producing laccase isolated from Gunung Pancar, Bogor, Indonesia. Biodiversitas 21: 2113-2118. Interpretation of phylogenetic trees is essential in understanding relationships between organisms, as well as their characteristics, bionetwork, and even their genomic and developmental ecology. The aim of this research is to analyze the phylogenetic bacteria producing laccase isolated from Gunung Pancar Bogor, Indonesia. Phylogenetic analysis of Geobacillus kaustophilus TP-02 producing laccase was performed using alignments with all Bacillus clusters. A phylogenetic tree was constructed by the neighbor-joining (NJ) method, using the maximum likelihood parameter. Laccase was purified using ammonium sulfate precipitation to 2.67-fold. The activity of crude laccase was determined to be 93.39 U/ml using 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as the substrate. Moreover, Geobacillus related to counting some Geobacillus genus such as Geobacillus thermocatenulatus, Geobacillus sp. MAS1, Geobacillus thermoleovorans, and Geobacillus zalihae, having similarity under 91% with comparison to the Geobacillus kaustophilius sequence in GenBank. Therefore, results show that G. kaustophilus TP-02 does not have any closeness with other Geobacillus genera, even though these species have the same ancestor.
format	article
author	WIN WIN MAR ALI ROHMAN NUR H. MUWAFIQI GALIH AYHUSTA LARAS DYAH AGUSTINA ONE ASMARANI NI NYOMAN TRI PUSPANINGSIH
author_facet	WIN WIN MAR ALI ROHMAN NUR H. MUWAFIQI GALIH AYHUSTA LARAS DYAH AGUSTINA ONE ASMARANI NI NYOMAN TRI PUSPANINGSIH
author_sort	WIN WIN MAR
title	Short Communication: Preliminary phylogenetic analysis of bacteria producing laccase isolated from Gunung Pancar, Bogor, Indonesia
title_short	Short Communication: Preliminary phylogenetic analysis of bacteria producing laccase isolated from Gunung Pancar, Bogor, Indonesia
title_full	Short Communication: Preliminary phylogenetic analysis of bacteria producing laccase isolated from Gunung Pancar, Bogor, Indonesia
title_fullStr	Short Communication: Preliminary phylogenetic analysis of bacteria producing laccase isolated from Gunung Pancar, Bogor, Indonesia
title_full_unstemmed	Short Communication: Preliminary phylogenetic analysis of bacteria producing laccase isolated from Gunung Pancar, Bogor, Indonesia
title_sort	short communication: preliminary phylogenetic analysis of bacteria producing laccase isolated from gunung pancar, bogor, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/2880e952bcb74c31adb0306c6a773ac4
work_keys_str_mv	AT winwinmar shortcommunicationpreliminaryphylogeneticanalysisofbacteriaproducinglaccaseisolatedfromgunungpancarbogorindonesia AT alirohman shortcommunicationpreliminaryphylogeneticanalysisofbacteriaproducinglaccaseisolatedfromgunungpancarbogorindonesia AT nurhmuwafiqi shortcommunicationpreliminaryphylogeneticanalysisofbacteriaproducinglaccaseisolatedfromgunungpancarbogorindonesia AT galihayhustalaras shortcommunicationpreliminaryphylogeneticanalysisofbacteriaproducinglaccaseisolatedfromgunungpancarbogorindonesia AT dyahagustina shortcommunicationpreliminaryphylogeneticanalysisofbacteriaproducinglaccaseisolatedfromgunungpancarbogorindonesia AT oneasmarani shortcommunicationpreliminaryphylogeneticanalysisofbacteriaproducinglaccaseisolatedfromgunungpancarbogorindonesia AT ninyomantripuspaningsih shortcommunicationpreliminaryphylogeneticanalysisofbacteriaproducinglaccaseisolatedfromgunungpancarbogorindonesia
_version_	1718418510961442816

Short Communication: Preliminary phylogenetic analysis of bacteria producing laccase isolated from Gunung Pancar, Bogor, Indonesia

Ejemplares similares