Autonomous lab-on-a-chip generic architecture for disposables with integrated actuation

Abstract The integration of actuators within disposable lab-on-a-chip devices is a demanding goal that requires reliable mechanisms, systematic fabrication procedures and marginal costs compatible with single-use devices. In this work an affordable 3D printed prototype that offers a compact and modu...

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Autores principales:	Anke Suska, Daniel Filippini
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/288252da4ae548f4ae4884bce52dce38
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spelling	oai:doaj.org-article:288252da4ae548f4ae4884bce52dce382021-12-02T15:12:22ZAutonomous lab-on-a-chip generic architecture for disposables with integrated actuation10.1038/s41598-019-55111-z2045-2322https://doaj.org/article/288252da4ae548f4ae4884bce52dce382019-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-55111-zhttps://doaj.org/toc/2045-2322Abstract The integration of actuators within disposable lab-on-a-chip devices is a demanding goal that requires reliable mechanisms, systematic fabrication procedures and marginal costs compatible with single-use devices. In this work an affordable 3D printed prototype that offers a compact and modular configuration to integrate actuation in autonomous lab-on-a-chip devices is demonstrated. The proposed concept can handle multiple step preparation protocols, such as the enzyme-linked immunosorbent assay (ELISA) configuration, by integrating reagents, volume metering capabilities with performance comparable to pipettes (e.g. 2.68% error for 5 μL volume), arbitrary dilution ratio support, effective mixing and active control of the sample injection. The chosen architecture is a manifold served by multiple injectors ending in unidirectional valves, which exchange a null dead volume when idle, thus isolating reagents until they are used. Functionalization is modularly provided by a plug-in element, which together with the selection of reagents can easily repurpose the platform to diverse targets, and this work demonstrates the systematic fabrication of 6 injectors/device at a development cost of USD$ 0.55/device. The concept was tested with a commercial ELISA kit for tumor necrosis factor (TNF), a marker for infectious, inflammatory and autoimmune disorders, and its performance satisfactorily compared with the classical microplate implementation.Anke SuskaDaniel FilippiniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anke Suska Daniel Filippini Autonomous lab-on-a-chip generic architecture for disposables with integrated actuation
description	Abstract The integration of actuators within disposable lab-on-a-chip devices is a demanding goal that requires reliable mechanisms, systematic fabrication procedures and marginal costs compatible with single-use devices. In this work an affordable 3D printed prototype that offers a compact and modular configuration to integrate actuation in autonomous lab-on-a-chip devices is demonstrated. The proposed concept can handle multiple step preparation protocols, such as the enzyme-linked immunosorbent assay (ELISA) configuration, by integrating reagents, volume metering capabilities with performance comparable to pipettes (e.g. 2.68% error for 5 μL volume), arbitrary dilution ratio support, effective mixing and active control of the sample injection. The chosen architecture is a manifold served by multiple injectors ending in unidirectional valves, which exchange a null dead volume when idle, thus isolating reagents until they are used. Functionalization is modularly provided by a plug-in element, which together with the selection of reagents can easily repurpose the platform to diverse targets, and this work demonstrates the systematic fabrication of 6 injectors/device at a development cost of USD$ 0.55/device. The concept was tested with a commercial ELISA kit for tumor necrosis factor (TNF), a marker for infectious, inflammatory and autoimmune disorders, and its performance satisfactorily compared with the classical microplate implementation.
format	article
author	Anke Suska Daniel Filippini
author_facet	Anke Suska Daniel Filippini
author_sort	Anke Suska
title	Autonomous lab-on-a-chip generic architecture for disposables with integrated actuation
title_short	Autonomous lab-on-a-chip generic architecture for disposables with integrated actuation
title_full	Autonomous lab-on-a-chip generic architecture for disposables with integrated actuation
title_fullStr	Autonomous lab-on-a-chip generic architecture for disposables with integrated actuation
title_full_unstemmed	Autonomous lab-on-a-chip generic architecture for disposables with integrated actuation
title_sort	autonomous lab-on-a-chip generic architecture for disposables with integrated actuation
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/288252da4ae548f4ae4884bce52dce38
work_keys_str_mv	AT ankesuska autonomouslabonachipgenericarchitecturefordisposableswithintegratedactuation AT danielfilippini autonomouslabonachipgenericarchitecturefordisposableswithintegratedactuation
_version_	1718387619051601920

Autonomous lab-on-a-chip generic architecture for disposables with integrated actuation

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