In vitro evolution of an influenza broadly neutralizing antibody is modulated by hemagglutinin receptor specificity

Broadly neutralizing antibodies (bnAbs) against influenza hemagglutinin (HA) have yielded insights for antiviral development. Here, the authors employ saturated mutagenesis of the paratope region of a bnAb combined with yeast display screening using H1 and H3 HAs, and find that a tradeoff exists bet...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nicholas C. Wu, Geramie Grande, Hannah L. Turner, Andrew B. Ward, Jia Xie, Richard A. Lerner, Ian A. Wilson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/288a464640f848a88e3527b72a95a760
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:288a464640f848a88e3527b72a95a760
record_format	dspace
spelling	oai:doaj.org-article:288a464640f848a88e3527b72a95a7602021-12-02T15:38:33ZIn vitro evolution of an influenza broadly neutralizing antibody is modulated by hemagglutinin receptor specificity10.1038/ncomms153712041-1723https://doaj.org/article/288a464640f848a88e3527b72a95a7602017-05-01T00:00:00Zhttps://doi.org/10.1038/ncomms15371https://doaj.org/toc/2041-1723Broadly neutralizing antibodies (bnAbs) against influenza hemagglutinin (HA) have yielded insights for antiviral development. Here, the authors employ saturated mutagenesis of the paratope region of a bnAb combined with yeast display screening using H1 and H3 HAs, and find that a tradeoff exists between Ab affinity and breadth that influenced by disparate modes of receptor binding.Nicholas C. WuGeramie GrandeHannah L. TurnerAndrew B. WardJia XieRichard A. LernerIan A. WilsonNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Nicholas C. Wu Geramie Grande Hannah L. Turner Andrew B. Ward Jia Xie Richard A. Lerner Ian A. Wilson In vitro evolution of an influenza broadly neutralizing antibody is modulated by hemagglutinin receptor specificity
description	Broadly neutralizing antibodies (bnAbs) against influenza hemagglutinin (HA) have yielded insights for antiviral development. Here, the authors employ saturated mutagenesis of the paratope region of a bnAb combined with yeast display screening using H1 and H3 HAs, and find that a tradeoff exists between Ab affinity and breadth that influenced by disparate modes of receptor binding.
format	article
author	Nicholas C. Wu Geramie Grande Hannah L. Turner Andrew B. Ward Jia Xie Richard A. Lerner Ian A. Wilson
author_facet	Nicholas C. Wu Geramie Grande Hannah L. Turner Andrew B. Ward Jia Xie Richard A. Lerner Ian A. Wilson
author_sort	Nicholas C. Wu
title	In vitro evolution of an influenza broadly neutralizing antibody is modulated by hemagglutinin receptor specificity
title_short	In vitro evolution of an influenza broadly neutralizing antibody is modulated by hemagglutinin receptor specificity
title_full	In vitro evolution of an influenza broadly neutralizing antibody is modulated by hemagglutinin receptor specificity
title_fullStr	In vitro evolution of an influenza broadly neutralizing antibody is modulated by hemagglutinin receptor specificity
title_full_unstemmed	In vitro evolution of an influenza broadly neutralizing antibody is modulated by hemagglutinin receptor specificity
title_sort	in vitro evolution of an influenza broadly neutralizing antibody is modulated by hemagglutinin receptor specificity
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/288a464640f848a88e3527b72a95a760
work_keys_str_mv	AT nicholascwu invitroevolutionofaninfluenzabroadlyneutralizingantibodyismodulatedbyhemagglutininreceptorspecificity AT geramiegrande invitroevolutionofaninfluenzabroadlyneutralizingantibodyismodulatedbyhemagglutininreceptorspecificity AT hannahlturner invitroevolutionofaninfluenzabroadlyneutralizingantibodyismodulatedbyhemagglutininreceptorspecificity AT andrewbward invitroevolutionofaninfluenzabroadlyneutralizingantibodyismodulatedbyhemagglutininreceptorspecificity AT jiaxie invitroevolutionofaninfluenzabroadlyneutralizingantibodyismodulatedbyhemagglutininreceptorspecificity AT richardalerner invitroevolutionofaninfluenzabroadlyneutralizingantibodyismodulatedbyhemagglutininreceptorspecificity AT ianawilson invitroevolutionofaninfluenzabroadlyneutralizingantibodyismodulatedbyhemagglutininreceptorspecificity
_version_	1718386161651548160

In vitro evolution of an influenza broadly neutralizing antibody is modulated by hemagglutinin receptor specificity

Ejemplares similares