Venom proteomic analysis of medically important Nigerian viper Echis ocellatus and Bitis arietans snake species

Snakebite envenoming remains a neglected tropical disease which poses severe health hazard, especially for the rural inhabitants in Africa. In Nigeria, vipers are responsible for the highest number of deaths. Hydrophilic interaction liquid chromatography coupled with LC-MS/MS was used to analyze the...

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Autores principales:	Emeka John Dingwoke, Fatima Amin Adamude, Gadija Mohamed, Ashwil Klein, Aliyu Salihu, Mujitaba Suleiman Abubakar, Abdullahi Balarabe Sallau
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Snakebite Neglected tropical disease Venom-based antivenom Viperidae Bitis arietans Echis ocellatus Biology (General) QH301-705.5 Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/2894289f57094a33b754f895690cf29f
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spelling	oai:doaj.org-article:2894289f57094a33b754f895690cf29f2021-11-04T04:33:07ZVenom proteomic analysis of medically important Nigerian viper Echis ocellatus and Bitis arietans snake species2405-580810.1016/j.bbrep.2021.101164https://doaj.org/article/2894289f57094a33b754f895690cf29f2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405580821002582https://doaj.org/toc/2405-5808Snakebite envenoming remains a neglected tropical disease which poses severe health hazard, especially for the rural inhabitants in Africa. In Nigeria, vipers are responsible for the highest number of deaths. Hydrophilic interaction liquid chromatography coupled with LC-MS/MS was used to analyze the crude venoms of Echis ocellatus (Carpet viper) and Bitis arietans (Puff adder) in order to understand their venom proteomic identities. Results obtained revealed that gel-free proteomic analysis of the crude venoms led to the identification of 85 and 79 proteins, respectively. Seventy-eight (78) proteins were common between the two snake species with a 91.8% similarity score. The identified proteins belong to 18 protein families in E. ocellatus and 14 protein families in B. arietans. Serine proteases (22.31%) and metalloproteinases (21.06%) were the dominant proteins in the venom of B. arietans; while metalloproteinases (34.84%), phospholipase A2s (21.19%) and serine proteases (15.50%) represent the major toxins in the E. ocellatus venom. Other protein families such as three-finger toxins and cysteine-rich venom proteins were detected in low proportions. This study provides an insight into the venom proteomic analysis of the two Nigerian viper species, which could be useful in identifying the toxin families to be neutralized in case of envenomation.Emeka John DingwokeFatima Amin AdamudeGadija MohamedAshwil KleinAliyu SalihuMujitaba Suleiman AbubakarAbdullahi Balarabe SallauElsevierarticleSnakebiteNeglected tropical diseaseVenom-based antivenomViperidaeBitis arietansEchis ocellatusBiology (General)QH301-705.5BiochemistryQD415-436ENBiochemistry and Biophysics Reports, Vol 28, Iss , Pp 101164- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Snakebite Neglected tropical disease Venom-based antivenom Viperidae Bitis arietans Echis ocellatus Biology (General) QH301-705.5 Biochemistry QD415-436
spellingShingle	Snakebite Neglected tropical disease Venom-based antivenom Viperidae Bitis arietans Echis ocellatus Biology (General) QH301-705.5 Biochemistry QD415-436 Emeka John Dingwoke Fatima Amin Adamude Gadija Mohamed Ashwil Klein Aliyu Salihu Mujitaba Suleiman Abubakar Abdullahi Balarabe Sallau Venom proteomic analysis of medically important Nigerian viper Echis ocellatus and Bitis arietans snake species
description	Snakebite envenoming remains a neglected tropical disease which poses severe health hazard, especially for the rural inhabitants in Africa. In Nigeria, vipers are responsible for the highest number of deaths. Hydrophilic interaction liquid chromatography coupled with LC-MS/MS was used to analyze the crude venoms of Echis ocellatus (Carpet viper) and Bitis arietans (Puff adder) in order to understand their venom proteomic identities. Results obtained revealed that gel-free proteomic analysis of the crude venoms led to the identification of 85 and 79 proteins, respectively. Seventy-eight (78) proteins were common between the two snake species with a 91.8% similarity score. The identified proteins belong to 18 protein families in E. ocellatus and 14 protein families in B. arietans. Serine proteases (22.31%) and metalloproteinases (21.06%) were the dominant proteins in the venom of B. arietans; while metalloproteinases (34.84%), phospholipase A2s (21.19%) and serine proteases (15.50%) represent the major toxins in the E. ocellatus venom. Other protein families such as three-finger toxins and cysteine-rich venom proteins were detected in low proportions. This study provides an insight into the venom proteomic analysis of the two Nigerian viper species, which could be useful in identifying the toxin families to be neutralized in case of envenomation.
format	article
author	Emeka John Dingwoke Fatima Amin Adamude Gadija Mohamed Ashwil Klein Aliyu Salihu Mujitaba Suleiman Abubakar Abdullahi Balarabe Sallau
author_facet	Emeka John Dingwoke Fatima Amin Adamude Gadija Mohamed Ashwil Klein Aliyu Salihu Mujitaba Suleiman Abubakar Abdullahi Balarabe Sallau
author_sort	Emeka John Dingwoke
title	Venom proteomic analysis of medically important Nigerian viper Echis ocellatus and Bitis arietans snake species
title_short	Venom proteomic analysis of medically important Nigerian viper Echis ocellatus and Bitis arietans snake species
title_full	Venom proteomic analysis of medically important Nigerian viper Echis ocellatus and Bitis arietans snake species
title_fullStr	Venom proteomic analysis of medically important Nigerian viper Echis ocellatus and Bitis arietans snake species
title_full_unstemmed	Venom proteomic analysis of medically important Nigerian viper Echis ocellatus and Bitis arietans snake species
title_sort	venom proteomic analysis of medically important nigerian viper echis ocellatus and bitis arietans snake species
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/2894289f57094a33b754f895690cf29f
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Venom proteomic analysis of medically important Nigerian viper Echis ocellatus and Bitis arietans snake species

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