IL-34 affects fibroblast-like synoviocyte proliferation, apoptosis and function by regulating IL-17

Abstract Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by proliferation and insufficient apoptosis of fibroblast-like synoviocytes (FLSs).The biology and functions of interleukin (IL)-34 are only beginning to be uncovered. We previously demonstrated IL-34 could upregulate...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xin Li, Yimeng Lei, Ziyu Gao, Gang Wu, Wei Gao, Liping Xia, Jing Lu, Hui Shen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2894796599fe4a90914b871836ba976a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2894796599fe4a90914b871836ba976a
record_format	dspace
spelling	oai:doaj.org-article:2894796599fe4a90914b871836ba976a2021-12-02T18:50:47ZIL-34 affects fibroblast-like synoviocyte proliferation, apoptosis and function by regulating IL-1710.1038/s41598-021-95839-12045-2322https://doaj.org/article/2894796599fe4a90914b871836ba976a2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95839-1https://doaj.org/toc/2045-2322Abstract Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by proliferation and insufficient apoptosis of fibroblast-like synoviocytes (FLSs).The biology and functions of interleukin (IL)-34 are only beginning to be uncovered. We previously demonstrated IL-34 could upregulate the expression of IL-17 in RA patients. In this study, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry of Annexin V and PI staining were performed to assess cell proliferation and apoptosis progression in RA-FLSs after stimulated with increasing concentrations of IL-34, respectively. Inflammatory cytokines and angiogenic factors were measured using quantitative real-time PCR, Western blotting and ELISA. We explored the association between IL-34 and RA-FLS proliferation and apoptosis in the context of RA. Stimulating RA-FLSs with different concentrations of IL-34 significantly promoted the proliferation and inhibited the apoptosis of RA-FLSs in a concentration-dependent manner. Neutralization of IL-17 with the IL-17 inhibitor plumbagin (PB) reduced the effects of IL-34. Proinflammatory cytokine (IL-17A IL-6 and tumor necrosis factor-α, TNF-α) and angiogenic factor (vascular endothelial growth factor, VEGF and hypoxia-inducible factor-1α, HIF-1α) expression was markedly upregulated in RA-FLSs stimulated by IL-34. PB-mediated inhibition of IL-17A also decreased the expression of IL-6, TNF-α, HIF-1α and VEGF in RA-FLSs. Taken together, these findings suggest that targeting IL-34 production in RA-FLSs may be a therapeutic strategy for RA.Xin LiYimeng LeiZiyu GaoGang WuWei GaoLiping XiaJing LuHui ShenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xin Li Yimeng Lei Ziyu Gao Gang Wu Wei Gao Liping Xia Jing Lu Hui Shen IL-34 affects fibroblast-like synoviocyte proliferation, apoptosis and function by regulating IL-17
description	Abstract Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by proliferation and insufficient apoptosis of fibroblast-like synoviocytes (FLSs).The biology and functions of interleukin (IL)-34 are only beginning to be uncovered. We previously demonstrated IL-34 could upregulate the expression of IL-17 in RA patients. In this study, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry of Annexin V and PI staining were performed to assess cell proliferation and apoptosis progression in RA-FLSs after stimulated with increasing concentrations of IL-34, respectively. Inflammatory cytokines and angiogenic factors were measured using quantitative real-time PCR, Western blotting and ELISA. We explored the association between IL-34 and RA-FLS proliferation and apoptosis in the context of RA. Stimulating RA-FLSs with different concentrations of IL-34 significantly promoted the proliferation and inhibited the apoptosis of RA-FLSs in a concentration-dependent manner. Neutralization of IL-17 with the IL-17 inhibitor plumbagin (PB) reduced the effects of IL-34. Proinflammatory cytokine (IL-17A IL-6 and tumor necrosis factor-α, TNF-α) and angiogenic factor (vascular endothelial growth factor, VEGF and hypoxia-inducible factor-1α, HIF-1α) expression was markedly upregulated in RA-FLSs stimulated by IL-34. PB-mediated inhibition of IL-17A also decreased the expression of IL-6, TNF-α, HIF-1α and VEGF in RA-FLSs. Taken together, these findings suggest that targeting IL-34 production in RA-FLSs may be a therapeutic strategy for RA.
format	article
author	Xin Li Yimeng Lei Ziyu Gao Gang Wu Wei Gao Liping Xia Jing Lu Hui Shen
author_facet	Xin Li Yimeng Lei Ziyu Gao Gang Wu Wei Gao Liping Xia Jing Lu Hui Shen
author_sort	Xin Li
title	IL-34 affects fibroblast-like synoviocyte proliferation, apoptosis and function by regulating IL-17
title_short	IL-34 affects fibroblast-like synoviocyte proliferation, apoptosis and function by regulating IL-17
title_full	IL-34 affects fibroblast-like synoviocyte proliferation, apoptosis and function by regulating IL-17
title_fullStr	IL-34 affects fibroblast-like synoviocyte proliferation, apoptosis and function by regulating IL-17
title_full_unstemmed	IL-34 affects fibroblast-like synoviocyte proliferation, apoptosis and function by regulating IL-17
title_sort	il-34 affects fibroblast-like synoviocyte proliferation, apoptosis and function by regulating il-17
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2894796599fe4a90914b871836ba976a
work_keys_str_mv	AT xinli il34affectsfibroblastlikesynoviocyteproliferationapoptosisandfunctionbyregulatingil17 AT yimenglei il34affectsfibroblastlikesynoviocyteproliferationapoptosisandfunctionbyregulatingil17 AT ziyugao il34affectsfibroblastlikesynoviocyteproliferationapoptosisandfunctionbyregulatingil17 AT gangwu il34affectsfibroblastlikesynoviocyteproliferationapoptosisandfunctionbyregulatingil17 AT weigao il34affectsfibroblastlikesynoviocyteproliferationapoptosisandfunctionbyregulatingil17 AT lipingxia il34affectsfibroblastlikesynoviocyteproliferationapoptosisandfunctionbyregulatingil17 AT jinglu il34affectsfibroblastlikesynoviocyteproliferationapoptosisandfunctionbyregulatingil17 AT huishen il34affectsfibroblastlikesynoviocyteproliferationapoptosisandfunctionbyregulatingil17
_version_	1718377492858798080

IL-34 affects fibroblast-like synoviocyte proliferation, apoptosis and function by regulating IL-17

Ejemplares similares