Circ-Usp10 promotes microglial activation and induces neuronal death by targeting miRNA-152-5p/CD84

Spinal cord injury (SCI) is a traumatic disease resulting in neuronal injury. circRNAs are closely associated with human diseases. Nevertheless, the potential mechanism by which circRNAs impact SCI remains to be elucidated. The aim of this study was to investigate the regulatory roles of Circular RN...

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Autores principales:	Dake Tong, Yanyin Zhao, Yang Tang, Jie Ma, Zhiwei Wang, Cheng Li
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	spinal cord injury (sci) microglial activation neuronal death circ-usp10 mir-152-5p Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/2897ceb04e15404aa0d6b708a57c5eb5
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spelling	oai:doaj.org-article:2897ceb04e15404aa0d6b708a57c5eb52021-11-11T14:23:43ZCirc-Usp10 promotes microglial activation and induces neuronal death by targeting miRNA-152-5p/CD842165-59792165-598710.1080/21655979.2021.2004362https://doaj.org/article/2897ceb04e15404aa0d6b708a57c5eb52021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2004362https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Spinal cord injury (SCI) is a traumatic disease resulting in neuronal injury. circRNAs are closely associated with human diseases. Nevertheless, the potential mechanism by which circRNAs impact SCI remains to be elucidated. The aim of this study was to investigate the regulatory roles of Circular RNAs (circRNAs) in SCI. The SCI mouse model and integrated bioinformatics analysis were used to identify the differentially expressed genes (DEGs). Functional enrichment analysis was conducted to study the related pathways. The circRNA-mediated ceRNA network and subnetwork was constructed based on circMir, TargetScan and miRanda. qRT–PCR, ELISA, flow cytometry, and luciferase reporter assays were carried out to validate the role of circ_0014637 (circ-Usp10) and microRNA(miR)-152-5p /CD84 in microglia. In all, 23 DE-circRNAs, 127 DE-miRNAs and 1327 DE-mRNAs were identified. We integrated these DEGs to construct a circRNA-miRNA-mRNA network. The circ-Usp10/miR-152-5p/CD84 axis was found to function in microglial activation. We also found that circ-Usp10 inhibited the secretion of proinflammatory factors in microglial BV2 cells. In addition, silencing circ-Usp10 significantly reduced the death of the neuronal cell line HT22. Taken together, we concluded that circ-Usp10 may function as a competing endogenous RNA (ceRNA) to promote microglial activation and induce neuronal death by targeting miR-152-5p/CD84. The circ-Usp10 may be a diagnostic biomarker and potential target for SCI therapy.Dake TongYanyin ZhaoYang TangJie MaZhiwei WangCheng LiTaylor & Francis Grouparticlespinal cord injury (sci)microglial activationneuronal deathcirc-usp10mir-152-5pBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	spinal cord injury (sci) microglial activation neuronal death circ-usp10 mir-152-5p Biotechnology TP248.13-248.65
spellingShingle	spinal cord injury (sci) microglial activation neuronal death circ-usp10 mir-152-5p Biotechnology TP248.13-248.65 Dake Tong Yanyin Zhao Yang Tang Jie Ma Zhiwei Wang Cheng Li Circ-Usp10 promotes microglial activation and induces neuronal death by targeting miRNA-152-5p/CD84
description	Spinal cord injury (SCI) is a traumatic disease resulting in neuronal injury. circRNAs are closely associated with human diseases. Nevertheless, the potential mechanism by which circRNAs impact SCI remains to be elucidated. The aim of this study was to investigate the regulatory roles of Circular RNAs (circRNAs) in SCI. The SCI mouse model and integrated bioinformatics analysis were used to identify the differentially expressed genes (DEGs). Functional enrichment analysis was conducted to study the related pathways. The circRNA-mediated ceRNA network and subnetwork was constructed based on circMir, TargetScan and miRanda. qRT–PCR, ELISA, flow cytometry, and luciferase reporter assays were carried out to validate the role of circ_0014637 (circ-Usp10) and microRNA(miR)-152-5p /CD84 in microglia. In all, 23 DE-circRNAs, 127 DE-miRNAs and 1327 DE-mRNAs were identified. We integrated these DEGs to construct a circRNA-miRNA-mRNA network. The circ-Usp10/miR-152-5p/CD84 axis was found to function in microglial activation. We also found that circ-Usp10 inhibited the secretion of proinflammatory factors in microglial BV2 cells. In addition, silencing circ-Usp10 significantly reduced the death of the neuronal cell line HT22. Taken together, we concluded that circ-Usp10 may function as a competing endogenous RNA (ceRNA) to promote microglial activation and induce neuronal death by targeting miR-152-5p/CD84. The circ-Usp10 may be a diagnostic biomarker and potential target for SCI therapy.
format	article
author	Dake Tong Yanyin Zhao Yang Tang Jie Ma Zhiwei Wang Cheng Li
author_facet	Dake Tong Yanyin Zhao Yang Tang Jie Ma Zhiwei Wang Cheng Li
author_sort	Dake Tong
title	Circ-Usp10 promotes microglial activation and induces neuronal death by targeting miRNA-152-5p/CD84
title_short	Circ-Usp10 promotes microglial activation and induces neuronal death by targeting miRNA-152-5p/CD84
title_full	Circ-Usp10 promotes microglial activation and induces neuronal death by targeting miRNA-152-5p/CD84
title_fullStr	Circ-Usp10 promotes microglial activation and induces neuronal death by targeting miRNA-152-5p/CD84
title_full_unstemmed	Circ-Usp10 promotes microglial activation and induces neuronal death by targeting miRNA-152-5p/CD84
title_sort	circ-usp10 promotes microglial activation and induces neuronal death by targeting mirna-152-5p/cd84
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/2897ceb04e15404aa0d6b708a57c5eb5
work_keys_str_mv	AT daketong circusp10promotesmicroglialactivationandinducesneuronaldeathbytargetingmirna1525pcd84 AT yanyinzhao circusp10promotesmicroglialactivationandinducesneuronaldeathbytargetingmirna1525pcd84 AT yangtang circusp10promotesmicroglialactivationandinducesneuronaldeathbytargetingmirna1525pcd84 AT jiema circusp10promotesmicroglialactivationandinducesneuronaldeathbytargetingmirna1525pcd84 AT zhiweiwang circusp10promotesmicroglialactivationandinducesneuronaldeathbytargetingmirna1525pcd84 AT chengli circusp10promotesmicroglialactivationandinducesneuronaldeathbytargetingmirna1525pcd84
_version_	1718438921020375040

Circ-Usp10 promotes microglial activation and induces neuronal death by targeting miRNA-152-5p/CD84

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