Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks

Climate change and global warming are leading to a change in weather patterns toward hot and cold waves. Citrus fruits are a tropical or subtropical crop whose growth is altered by changes in weather patterns. Thus, in the present work, two experiments are evaluated to obtain a screening method to s...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Amparo Primo-Capella, Mary-Rus Martínez-Cuenca, Maria Ángeles Forner-Giner
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	citrus rootstock cold stress breeding program abiotic stress Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/2898a83174fc4e6aabdf01c7fcb36738
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2898a83174fc4e6aabdf01c7fcb36738
record_format	dspace
spelling	oai:doaj.org-article:2898a83174fc4e6aabdf01c7fcb367382021-11-25T17:47:14ZGene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks10.3390/horticulturae71104472311-7524https://doaj.org/article/2898a83174fc4e6aabdf01c7fcb367382021-11-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/447https://doaj.org/toc/2311-7524Climate change and global warming are leading to a change in weather patterns toward hot and cold waves. Citrus fruits are a tropical or subtropical crop whose growth is altered by changes in weather patterns. Thus, in the present work, two experiments are evaluated to obtain a screening method to select citrus rootstocks that help us to select new low-temperature-tolerant plant materials. One cold experiment was carried out with the <i>Poncirus trifoliata</i> and <i>Citrus macrophylla</i> rootstocks at 4 °C for 4, 8, 24 and 56 h. A second experiment was performed at 4 °C for 5 days with subsequent acclimatization lasting 0, 5, 10 and 24 h. The expression of the cold response genes <i>CAMTA1, CAMTA3, CAMTA5, CBF1, ICE1</i> and <i>COR413 IM1</i> was quantified. The results showed that the best rootstock selection strategy was the second experiment, as a higher expression of the genes <i>CAMTA3, CAMTA5, CBF1</i> and <i>COR413 IM1</i> was seen in the tolerant genotype <i>P. trifoliata</i>. We quantified the gene expression of proline biosynthesis <i>P5CS1, dOAT</i> and the proline transporters <i>PROT1</i> and <i>PROT2</i>; the concentration of the amino acid proline in leaves was also quantified. These results once again showed that the best experiment to differentiate between tolerant and sensitive rootstocks was the second experiment with acclimation time.Amparo Primo-CapellaMary-Rus Martínez-CuencaMaria Ángeles Forner-GinerMDPI AGarticlecitrusrootstockcold stressbreeding programabiotic stressPlant cultureSB1-1110ENHorticulturae, Vol 7, Iss 447, p 447 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	citrus rootstock cold stress breeding program abiotic stress Plant culture SB1-1110
spellingShingle	citrus rootstock cold stress breeding program abiotic stress Plant culture SB1-1110 Amparo Primo-Capella Mary-Rus Martínez-Cuenca Maria Ángeles Forner-Giner Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks
description	Climate change and global warming are leading to a change in weather patterns toward hot and cold waves. Citrus fruits are a tropical or subtropical crop whose growth is altered by changes in weather patterns. Thus, in the present work, two experiments are evaluated to obtain a screening method to select citrus rootstocks that help us to select new low-temperature-tolerant plant materials. One cold experiment was carried out with the <i>Poncirus trifoliata</i> and <i>Citrus macrophylla</i> rootstocks at 4 °C for 4, 8, 24 and 56 h. A second experiment was performed at 4 °C for 5 days with subsequent acclimatization lasting 0, 5, 10 and 24 h. The expression of the cold response genes <i>CAMTA1, CAMTA3, CAMTA5, CBF1, ICE1</i> and <i>COR413 IM1</i> was quantified. The results showed that the best rootstock selection strategy was the second experiment, as a higher expression of the genes <i>CAMTA3, CAMTA5, CBF1</i> and <i>COR413 IM1</i> was seen in the tolerant genotype <i>P. trifoliata</i>. We quantified the gene expression of proline biosynthesis <i>P5CS1, dOAT</i> and the proline transporters <i>PROT1</i> and <i>PROT2</i>; the concentration of the amino acid proline in leaves was also quantified. These results once again showed that the best experiment to differentiate between tolerant and sensitive rootstocks was the second experiment with acclimation time.
format	article
author	Amparo Primo-Capella Mary-Rus Martínez-Cuenca Maria Ángeles Forner-Giner
author_facet	Amparo Primo-Capella Mary-Rus Martínez-Cuenca Maria Ángeles Forner-Giner
author_sort	Amparo Primo-Capella
title	Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks
title_short	Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks
title_full	Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks
title_fullStr	Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks
title_full_unstemmed	Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks
title_sort	gene expression under short-term low temperatures: preliminary screening method to obtain tolerant citrus rootstocks
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2898a83174fc4e6aabdf01c7fcb36738
work_keys_str_mv	AT amparoprimocapella geneexpressionundershorttermlowtemperaturespreliminaryscreeningmethodtoobtaintolerantcitrusrootstocks AT maryrusmartinezcuenca geneexpressionundershorttermlowtemperaturespreliminaryscreeningmethodtoobtaintolerantcitrusrootstocks AT mariaangelesfornerginer geneexpressionundershorttermlowtemperaturespreliminaryscreeningmethodtoobtaintolerantcitrusrootstocks
_version_	1718412033166147584

Gene Expression under Short-Term Low Temperatures: Preliminary Screening Method to Obtain Tolerant Citrus Rootstocks

Ejemplares similares