On the relationship between sialomucin and sulfomucin expression and hydrogenotrophic microbes in the human colonic mucosa.

The colonic mucus layer is comprised primarily of acidomucins, which provide viscous properties and can be broadly classified into sialomucins or sulfomucins based on the presence of terminating sialic acid or sulfate groups. Differences in acidomucin chemotypes have been observed in diseases such a...

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Autores principales:	Jennifer A Croix, Franck Carbonero, Gerardo M Nava, Mark Russell, Eugene Greenberg, H Rex Gaskins
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/28a39dcef7ff420cac0cac00041413f0
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spelling	oai:doaj.org-article:28a39dcef7ff420cac0cac00041413f02021-11-04T06:08:54ZOn the relationship between sialomucin and sulfomucin expression and hydrogenotrophic microbes in the human colonic mucosa.1932-620310.1371/journal.pone.0024447https://doaj.org/article/28a39dcef7ff420cac0cac00041413f02011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21931721/?tool=EBIhttps://doaj.org/toc/1932-6203The colonic mucus layer is comprised primarily of acidomucins, which provide viscous properties and can be broadly classified into sialomucins or sulfomucins based on the presence of terminating sialic acid or sulfate groups. Differences in acidomucin chemotypes have been observed in diseases such as colorectal cancer and inflammatory bowel disease, and variation in sialo- and sulfomucin content may influence microbial colonization. For example, sulfate derived from sulfomucin degradation may promote the colonization of sulfate-reducing bacteria (SRB), which through sulfate respiration generate the genotoxic gas hydrogen sulfide. Here, paired biopsies from right colon, left colon, and rectum of 20 subjects undergoing routine screening colonoscopies were collected to enable parallel histochemical and microbiological studies. Goblet cell sialo- and sulfomucins in each biopsy were distinguished histochemically and quantified. Quantitative PCR and multivariate analyses were used to examine the abundance of hydrogenotrophic microbial groups and SRB genera relative to acidomucin profiles. Regional variation was observed in sialomucins and sulfomucins with the greatest abundance of each found in the rectum. Mucin composition did not appear to influence the abundance of SRB or other hydrogenotrophic microbiota but correlated with the composition of different SRB genera. A higher sulfomucin proportion correlated with higher quantities of Desulfobacter, Desulfobulbus and Desulfotomaculum, relative to the predominant Desulfovibrio genus. Thus, acidomucin composition may influence bacterial sulfate respiration in the human colon, which may in turn impact mucosal homeostasis. These results stress the need to consider mucus characteristics in the context of studies of the microbiome that target intestinal diseases.Jennifer A CroixFranck CarboneroGerardo M NavaMark RussellEugene GreenbergH Rex GaskinsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 9, p e24447 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jennifer A Croix Franck Carbonero Gerardo M Nava Mark Russell Eugene Greenberg H Rex Gaskins On the relationship between sialomucin and sulfomucin expression and hydrogenotrophic microbes in the human colonic mucosa.
description	The colonic mucus layer is comprised primarily of acidomucins, which provide viscous properties and can be broadly classified into sialomucins or sulfomucins based on the presence of terminating sialic acid or sulfate groups. Differences in acidomucin chemotypes have been observed in diseases such as colorectal cancer and inflammatory bowel disease, and variation in sialo- and sulfomucin content may influence microbial colonization. For example, sulfate derived from sulfomucin degradation may promote the colonization of sulfate-reducing bacteria (SRB), which through sulfate respiration generate the genotoxic gas hydrogen sulfide. Here, paired biopsies from right colon, left colon, and rectum of 20 subjects undergoing routine screening colonoscopies were collected to enable parallel histochemical and microbiological studies. Goblet cell sialo- and sulfomucins in each biopsy were distinguished histochemically and quantified. Quantitative PCR and multivariate analyses were used to examine the abundance of hydrogenotrophic microbial groups and SRB genera relative to acidomucin profiles. Regional variation was observed in sialomucins and sulfomucins with the greatest abundance of each found in the rectum. Mucin composition did not appear to influence the abundance of SRB or other hydrogenotrophic microbiota but correlated with the composition of different SRB genera. A higher sulfomucin proportion correlated with higher quantities of Desulfobacter, Desulfobulbus and Desulfotomaculum, relative to the predominant Desulfovibrio genus. Thus, acidomucin composition may influence bacterial sulfate respiration in the human colon, which may in turn impact mucosal homeostasis. These results stress the need to consider mucus characteristics in the context of studies of the microbiome that target intestinal diseases.
format	article
author	Jennifer A Croix Franck Carbonero Gerardo M Nava Mark Russell Eugene Greenberg H Rex Gaskins
author_facet	Jennifer A Croix Franck Carbonero Gerardo M Nava Mark Russell Eugene Greenberg H Rex Gaskins
author_sort	Jennifer A Croix
title	On the relationship between sialomucin and sulfomucin expression and hydrogenotrophic microbes in the human colonic mucosa.
title_short	On the relationship between sialomucin and sulfomucin expression and hydrogenotrophic microbes in the human colonic mucosa.
title_full	On the relationship between sialomucin and sulfomucin expression and hydrogenotrophic microbes in the human colonic mucosa.
title_fullStr	On the relationship between sialomucin and sulfomucin expression and hydrogenotrophic microbes in the human colonic mucosa.
title_full_unstemmed	On the relationship between sialomucin and sulfomucin expression and hydrogenotrophic microbes in the human colonic mucosa.
title_sort	on the relationship between sialomucin and sulfomucin expression and hydrogenotrophic microbes in the human colonic mucosa.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/28a39dcef7ff420cac0cac00041413f0
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On the relationship between sialomucin and sulfomucin expression and hydrogenotrophic microbes in the human colonic mucosa.

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