Cellular uptake pathways of sepiolite nanofibers and DNA transfection improvement

Abstract Sepiolite is a nanofibrous natural silicate that can be used as a nanocarrier because it can be naturally internalized into mammalian cells, due to its nano-size dimension. Therefore, deciphering the mechanisms of sepiolite cell internalization constitutes a question interesting biotechnolo...

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Autores principales:	Fidel Antonio Castro-Smirnov, Jeanne Ayache, Jean-Rémi Bertrand, Elodie Dardillac, Eric Le Cam, Olivier Piétrement, Pilar Aranda, Eduardo Ruiz-Hitzky, Bernard S. Lopez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/28a9a03e50bd4d2ab47968ae01acba58
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spelling	oai:doaj.org-article:28a9a03e50bd4d2ab47968ae01acba582021-12-02T12:31:45ZCellular uptake pathways of sepiolite nanofibers and DNA transfection improvement10.1038/s41598-017-05839-32045-2322https://doaj.org/article/28a9a03e50bd4d2ab47968ae01acba582017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05839-3https://doaj.org/toc/2045-2322Abstract Sepiolite is a nanofibrous natural silicate that can be used as a nanocarrier because it can be naturally internalized into mammalian cells, due to its nano-size dimension. Therefore, deciphering the mechanisms of sepiolite cell internalization constitutes a question interesting biotechnology, for the use of sepiolite as nanocarrier, as well as environmental and public health concerns. Though it is low, the perfectly stable and natural intrinsic fluorescence of sepiolite nanofibers allows to follow their fate into cells by specifically sensitive technics. By combining fluorescence microscopy (including confocal analysis), time-lapse video microscopy, fluorescence activated cell sorting and transmission electron microscopy, we show that sepiolite can be spontaneously internalized into mammalian cells through both non-endocytic and endocytic pathways, macropinocytosis being one of the main pathways. Interestingly, exposure of the cells to endocytosis inhibitors, such as chloroquine, two-fold increase the efficiency of sepiolite-mediated gene transfer, in addition to the 100-fold increased resulting from sepiolite sonomechanical treatment. As sepiolite is able to bind various biological molecules, this nanoparticulate silicate could be a good candidate as a nanocarrier for simultaneous vectorization of diverse biological molecules.Fidel Antonio Castro-SmirnovJeanne AyacheJean-Rémi BertrandElodie DardillacEric Le CamOlivier PiétrementPilar ArandaEduardo Ruiz-HitzkyBernard S. LopezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fidel Antonio Castro-Smirnov Jeanne Ayache Jean-Rémi Bertrand Elodie Dardillac Eric Le Cam Olivier Piétrement Pilar Aranda Eduardo Ruiz-Hitzky Bernard S. Lopez Cellular uptake pathways of sepiolite nanofibers and DNA transfection improvement
description	Abstract Sepiolite is a nanofibrous natural silicate that can be used as a nanocarrier because it can be naturally internalized into mammalian cells, due to its nano-size dimension. Therefore, deciphering the mechanisms of sepiolite cell internalization constitutes a question interesting biotechnology, for the use of sepiolite as nanocarrier, as well as environmental and public health concerns. Though it is low, the perfectly stable and natural intrinsic fluorescence of sepiolite nanofibers allows to follow their fate into cells by specifically sensitive technics. By combining fluorescence microscopy (including confocal analysis), time-lapse video microscopy, fluorescence activated cell sorting and transmission electron microscopy, we show that sepiolite can be spontaneously internalized into mammalian cells through both non-endocytic and endocytic pathways, macropinocytosis being one of the main pathways. Interestingly, exposure of the cells to endocytosis inhibitors, such as chloroquine, two-fold increase the efficiency of sepiolite-mediated gene transfer, in addition to the 100-fold increased resulting from sepiolite sonomechanical treatment. As sepiolite is able to bind various biological molecules, this nanoparticulate silicate could be a good candidate as a nanocarrier for simultaneous vectorization of diverse biological molecules.
format	article
author	Fidel Antonio Castro-Smirnov Jeanne Ayache Jean-Rémi Bertrand Elodie Dardillac Eric Le Cam Olivier Piétrement Pilar Aranda Eduardo Ruiz-Hitzky Bernard S. Lopez
author_facet	Fidel Antonio Castro-Smirnov Jeanne Ayache Jean-Rémi Bertrand Elodie Dardillac Eric Le Cam Olivier Piétrement Pilar Aranda Eduardo Ruiz-Hitzky Bernard S. Lopez
author_sort	Fidel Antonio Castro-Smirnov
title	Cellular uptake pathways of sepiolite nanofibers and DNA transfection improvement
title_short	Cellular uptake pathways of sepiolite nanofibers and DNA transfection improvement
title_full	Cellular uptake pathways of sepiolite nanofibers and DNA transfection improvement
title_fullStr	Cellular uptake pathways of sepiolite nanofibers and DNA transfection improvement
title_full_unstemmed	Cellular uptake pathways of sepiolite nanofibers and DNA transfection improvement
title_sort	cellular uptake pathways of sepiolite nanofibers and dna transfection improvement
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/28a9a03e50bd4d2ab47968ae01acba58
work_keys_str_mv	AT fidelantoniocastrosmirnov cellularuptakepathwaysofsepiolitenanofibersanddnatransfectionimprovement AT jeanneayache cellularuptakepathwaysofsepiolitenanofibersanddnatransfectionimprovement AT jeanremibertrand cellularuptakepathwaysofsepiolitenanofibersanddnatransfectionimprovement AT elodiedardillac cellularuptakepathwaysofsepiolitenanofibersanddnatransfectionimprovement AT ericlecam cellularuptakepathwaysofsepiolitenanofibersanddnatransfectionimprovement AT olivierpietrement cellularuptakepathwaysofsepiolitenanofibersanddnatransfectionimprovement AT pilararanda cellularuptakepathwaysofsepiolitenanofibersanddnatransfectionimprovement AT eduardoruizhitzky cellularuptakepathwaysofsepiolitenanofibersanddnatransfectionimprovement AT bernardslopez cellularuptakepathwaysofsepiolitenanofibersanddnatransfectionimprovement
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Cellular uptake pathways of sepiolite nanofibers and DNA transfection improvement

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