Chlorite oxidized oxyamylose differentially influences the microstructure of fibrin and self assembling peptide hydrogels as well as dental pulp stem cell behavior

Abstract Tailored hydrogels mimicking the native extracellular environment could help overcome the high variability in outcomes within regenerative endodontics. This study aimed to evaluate the effect of the chemokine-binding and antimicrobial polymer, chlorite-oxidized oxyamylose (COAM), on the mic...

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Autores principales:	Mostafa EzEldeen, Burak Toprakhisar, Denise Murgia, Nick Smisdom, Olivier Deschaume, Carmen Bartic, Hans Van Oosterwyck, Rafaela Vaz Sousa Pereira, Ghislain Opdenakker, Ivo Lambrichts, Annelies Bronckaers, Reinhilde Jacobs, Jennifer Patterson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/28aa3ba4bf784093af58d527a792cfcc
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spelling	oai:doaj.org-article:28aa3ba4bf784093af58d527a792cfcc2021-12-02T13:20:02ZChlorite oxidized oxyamylose differentially influences the microstructure of fibrin and self assembling peptide hydrogels as well as dental pulp stem cell behavior10.1038/s41598-021-84405-42045-2322https://doaj.org/article/28aa3ba4bf784093af58d527a792cfcc2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84405-4https://doaj.org/toc/2045-2322Abstract Tailored hydrogels mimicking the native extracellular environment could help overcome the high variability in outcomes within regenerative endodontics. This study aimed to evaluate the effect of the chemokine-binding and antimicrobial polymer, chlorite-oxidized oxyamylose (COAM), on the microstructural properties of fibrin and self-assembling peptide (SAP) hydrogels. A further goal was to assess the influence of the microstructural differences between the hydrogels on the in vitro behavior of human dental pulp stem cells (hDPSCs). Structural and mechanical characterization of the hydrogels with and without COAM was performed by atomic force microscopy and scanning electron microscopy to characterize their microstructure (roughness and fiber length, diameter, straightness, and alignment) and by nanoindentation to measure their stiffness (elastic modulus). Then, hDPSCs were encapsulated in hydrogels with and without COAM. Cell viability and circularity were determined using confocal microscopy, and proliferation was determined using DNA quantification. Inclusion of COAM did not alter the microstructure of the fibrin hydrogels at the fiber level while affecting the SAP hydrogel microstructure (homogeneity), leading to fiber aggregation. The stiffness of the SAP hydrogels was sevenfold higher than the fibrin hydrogels. The viability and attachment of hDPSCs were significantly higher in fibrin hydrogels than in SAP hydrogels. The DNA content was significantly affected by the hydrogel type and the presence of COAM. The microstructural stability after COAM inclusion and the favorable hDPSCs' response observed in fibrin hydrogels suggest this system as a promising carrier for COAM and application in endodontic regeneration.Mostafa EzEldeenBurak ToprakhisarDenise MurgiaNick SmisdomOlivier DeschaumeCarmen BarticHans Van OosterwyckRafaela Vaz Sousa PereiraGhislain OpdenakkerIvo LambrichtsAnnelies BronckaersReinhilde JacobsJennifer PattersonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mostafa EzEldeen Burak Toprakhisar Denise Murgia Nick Smisdom Olivier Deschaume Carmen Bartic Hans Van Oosterwyck Rafaela Vaz Sousa Pereira Ghislain Opdenakker Ivo Lambrichts Annelies Bronckaers Reinhilde Jacobs Jennifer Patterson Chlorite oxidized oxyamylose differentially influences the microstructure of fibrin and self assembling peptide hydrogels as well as dental pulp stem cell behavior
description	Abstract Tailored hydrogels mimicking the native extracellular environment could help overcome the high variability in outcomes within regenerative endodontics. This study aimed to evaluate the effect of the chemokine-binding and antimicrobial polymer, chlorite-oxidized oxyamylose (COAM), on the microstructural properties of fibrin and self-assembling peptide (SAP) hydrogels. A further goal was to assess the influence of the microstructural differences between the hydrogels on the in vitro behavior of human dental pulp stem cells (hDPSCs). Structural and mechanical characterization of the hydrogels with and without COAM was performed by atomic force microscopy and scanning electron microscopy to characterize their microstructure (roughness and fiber length, diameter, straightness, and alignment) and by nanoindentation to measure their stiffness (elastic modulus). Then, hDPSCs were encapsulated in hydrogels with and without COAM. Cell viability and circularity were determined using confocal microscopy, and proliferation was determined using DNA quantification. Inclusion of COAM did not alter the microstructure of the fibrin hydrogels at the fiber level while affecting the SAP hydrogel microstructure (homogeneity), leading to fiber aggregation. The stiffness of the SAP hydrogels was sevenfold higher than the fibrin hydrogels. The viability and attachment of hDPSCs were significantly higher in fibrin hydrogels than in SAP hydrogels. The DNA content was significantly affected by the hydrogel type and the presence of COAM. The microstructural stability after COAM inclusion and the favorable hDPSCs' response observed in fibrin hydrogels suggest this system as a promising carrier for COAM and application in endodontic regeneration.
format	article
author	Mostafa EzEldeen Burak Toprakhisar Denise Murgia Nick Smisdom Olivier Deschaume Carmen Bartic Hans Van Oosterwyck Rafaela Vaz Sousa Pereira Ghislain Opdenakker Ivo Lambrichts Annelies Bronckaers Reinhilde Jacobs Jennifer Patterson
author_facet	Mostafa EzEldeen Burak Toprakhisar Denise Murgia Nick Smisdom Olivier Deschaume Carmen Bartic Hans Van Oosterwyck Rafaela Vaz Sousa Pereira Ghislain Opdenakker Ivo Lambrichts Annelies Bronckaers Reinhilde Jacobs Jennifer Patterson
author_sort	Mostafa EzEldeen
title	Chlorite oxidized oxyamylose differentially influences the microstructure of fibrin and self assembling peptide hydrogels as well as dental pulp stem cell behavior
title_short	Chlorite oxidized oxyamylose differentially influences the microstructure of fibrin and self assembling peptide hydrogels as well as dental pulp stem cell behavior
title_full	Chlorite oxidized oxyamylose differentially influences the microstructure of fibrin and self assembling peptide hydrogels as well as dental pulp stem cell behavior
title_fullStr	Chlorite oxidized oxyamylose differentially influences the microstructure of fibrin and self assembling peptide hydrogels as well as dental pulp stem cell behavior
title_full_unstemmed	Chlorite oxidized oxyamylose differentially influences the microstructure of fibrin and self assembling peptide hydrogels as well as dental pulp stem cell behavior
title_sort	chlorite oxidized oxyamylose differentially influences the microstructure of fibrin and self assembling peptide hydrogels as well as dental pulp stem cell behavior
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/28aa3ba4bf784093af58d527a792cfcc
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Chlorite oxidized oxyamylose differentially influences the microstructure of fibrin and self assembling peptide hydrogels as well as dental pulp stem cell behavior

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