Evaluation Of Using Different Adjuvants Used For Preparation Of Staphylococcus Aureus Mastitis Vaccine On The Immune Response

Staphylococcus aureus (S. aureus) was the most prevalent bacteria recorded from clinical and subclinical mastitis. The main objective of the present work is to prepare and evaluate the effectiveness of locally prepared inactivated S. aureus vaccine using different adjuvants Montanide ISA-206, Montan...

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Autores principales:	Ebtsam E.Z. Kotb, Amal M. EL Sawah, Laila E. Kortam, Ola A. Abd El Fattah, Waleed M. Barghooth
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2021
Materias:	adjuvant mastitis staphylococcus aureus vaccine Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2021.140065 https://doaj.org/article/28b3a279f19f4a02a55bd8c1da6ad8e4
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spelling	oai:doaj.org-article:28b3a279f19f4a02a55bd8c1da6ad8e42021-12-02T13:24:18ZEvaluation Of Using Different Adjuvants Used For Preparation Of Staphylococcus Aureus Mastitis Vaccine On The Immune Responsehttps://dx.doi.org/10.21608/javs.2021.1400651687-40722090-3308https://doaj.org/article/28b3a279f19f4a02a55bd8c1da6ad8e42021-01-01T00:00:00Zhttps://javs.journals.ekb.eg/article_140065.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308Staphylococcus aureus (S. aureus) was the most prevalent bacteria recorded from clinical and subclinical mastitis. The main objective of the present work is to prepare and evaluate the effectiveness of locally prepared inactivated S. aureus vaccine using different adjuvants Montanide ISA-206, Montanide ISA-70 and alum hydroxide gel for detection of cellular and humeral immune responses. Seventy dairy heifers in the last trimester of gestation divided into (5) groups. Twenty pregnant cows in three groups (G1, G2, and G3) were inoculated intramuscularly with the prepared vaccines four weeks prior to calving then a booster dose at two weeks pre-expected calving. The animals in remaining groups (G4 and G5) were considered as control and safety test respectively. Serum samples from vaccinated and non-vaccinated cows were collected at the 1st and 2nd immunization then at 0, 1, 2, 3 months and (7, 10, 14) days post calving and vaccination and evaluated immunologically using ELISA and Lymphocyte transformation assay, respectively. The results revealed that a progressive increase of immune response in the vaccinated groups (G1 and G2) than that of the third and control groups. These results could be indicated the safety and effectiveness of the Montanide ISA-206, ISA-70 vaccines than alum adjuvant vaccine that reduce the incidence and severity of clinical cases of mastitis. Hence, further studies should be done to elucidate the possibility of field application.Ebtsam E.Z. KotbAmal M. EL SawahLaila E. KortamOla A. Abd El FattahWaleed M. BarghoothEgyptian Society for Animal Managementarticleadjuvantmastitisstaphylococcus aureusvaccineZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 6, Iss 1, Pp 9-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adjuvant mastitis staphylococcus aureus vaccine Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	adjuvant mastitis staphylococcus aureus vaccine Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Ebtsam E.Z. Kotb Amal M. EL Sawah Laila E. Kortam Ola A. Abd El Fattah Waleed M. Barghooth Evaluation Of Using Different Adjuvants Used For Preparation Of Staphylococcus Aureus Mastitis Vaccine On The Immune Response
description	Staphylococcus aureus (S. aureus) was the most prevalent bacteria recorded from clinical and subclinical mastitis. The main objective of the present work is to prepare and evaluate the effectiveness of locally prepared inactivated S. aureus vaccine using different adjuvants Montanide ISA-206, Montanide ISA-70 and alum hydroxide gel for detection of cellular and humeral immune responses. Seventy dairy heifers in the last trimester of gestation divided into (5) groups. Twenty pregnant cows in three groups (G1, G2, and G3) were inoculated intramuscularly with the prepared vaccines four weeks prior to calving then a booster dose at two weeks pre-expected calving. The animals in remaining groups (G4 and G5) were considered as control and safety test respectively. Serum samples from vaccinated and non-vaccinated cows were collected at the 1st and 2nd immunization then at 0, 1, 2, 3 months and (7, 10, 14) days post calving and vaccination and evaluated immunologically using ELISA and Lymphocyte transformation assay, respectively. The results revealed that a progressive increase of immune response in the vaccinated groups (G1 and G2) than that of the third and control groups. These results could be indicated the safety and effectiveness of the Montanide ISA-206, ISA-70 vaccines than alum adjuvant vaccine that reduce the incidence and severity of clinical cases of mastitis. Hence, further studies should be done to elucidate the possibility of field application.
format	article
author	Ebtsam E.Z. Kotb Amal M. EL Sawah Laila E. Kortam Ola A. Abd El Fattah Waleed M. Barghooth
author_facet	Ebtsam E.Z. Kotb Amal M. EL Sawah Laila E. Kortam Ola A. Abd El Fattah Waleed M. Barghooth
author_sort	Ebtsam E.Z. Kotb
title	Evaluation Of Using Different Adjuvants Used For Preparation Of Staphylococcus Aureus Mastitis Vaccine On The Immune Response
title_short	Evaluation Of Using Different Adjuvants Used For Preparation Of Staphylococcus Aureus Mastitis Vaccine On The Immune Response
title_full	Evaluation Of Using Different Adjuvants Used For Preparation Of Staphylococcus Aureus Mastitis Vaccine On The Immune Response
title_fullStr	Evaluation Of Using Different Adjuvants Used For Preparation Of Staphylococcus Aureus Mastitis Vaccine On The Immune Response
title_full_unstemmed	Evaluation Of Using Different Adjuvants Used For Preparation Of Staphylococcus Aureus Mastitis Vaccine On The Immune Response
title_sort	evaluation of using different adjuvants used for preparation of staphylococcus aureus mastitis vaccine on the immune response
publisher	Egyptian Society for Animal Management
publishDate	2021
url	https://dx.doi.org/10.21608/javs.2021.140065 https://doaj.org/article/28b3a279f19f4a02a55bd8c1da6ad8e4
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Evaluation Of Using Different Adjuvants Used For Preparation Of Staphylococcus Aureus Mastitis Vaccine On The Immune Response

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