Molecular characterization of circulating tumor cells in human metastatic colorectal cancer.

Metastatic colorectal cancer (mCRC) relies on the detachment of aggressive malignant cells from the primary tumor into the bloodstream and, concordantly, the presence of these Circulating Tumor Cells (CTC) is associated with a poor prognosis. In this work, the molecular characterization of CTC from...

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Autores principales:	Jorge Barbazán, Lorena Alonso-Alconada, Laura Muinelo-Romay, María Vieito, Alicia Abalo, Marta Alonso-Nocelo, Sonia Candamio, Elena Gallardo, Beatriz Fernández, Ihab Abdulkader, María de Los Ángeles Casares, Antonio Gómez-Tato, Rafael López-López, Miguel Abal
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/28b3f3215fb94b26a6060573ce85ac50
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spelling	oai:doaj.org-article:28b3f3215fb94b26a6060573ce85ac502021-11-18T07:12:53ZMolecular characterization of circulating tumor cells in human metastatic colorectal cancer.1932-620310.1371/journal.pone.0040476https://doaj.org/article/28b3f3215fb94b26a6060573ce85ac502012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22811761/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Metastatic colorectal cancer (mCRC) relies on the detachment of aggressive malignant cells from the primary tumor into the bloodstream and, concordantly, the presence of these Circulating Tumor Cells (CTC) is associated with a poor prognosis. In this work, the molecular characterization of CTC from mCRC patients was approached, with the aim of understanding their biology and improving their clinical utility in the management of colorectal cancer patients. For this, EpCAM-based immunoisolation of CTC was combined with whole transcriptome amplification and hybridization onto cDNA microarrays. Gene expression data from mCRC patients, once the background of unspecific immunoisolation from a group of controls had been subtracted, resulted in 410 genes that characterized the CTC population. Bioinformatics were used for the biological interpretation of the data, revealing that CTC are characterized by genes related to cell movement and adhesion, cell death and proliferation, and cell signalling and interaction. RTqPCR on an independent series of mCRC patients and controls was used for the validation of a number of genes related to the main cellular functions characterizing the CTC population. Comparison between primary carcinomas and lung and liver metastases further involved the CTC-genes in the promotion of metastasis. Moreover, the correlation of CTC-gene expression with clinical parameters demonstrated detection and prognosis significance. In conclusion, the molecular characterization of CTC from mCRC patients and the identification of diagnostic and prognostic biomarkers represent an innovative and promising approach in the clinical management of this type of patients.Jorge BarbazánLorena Alonso-AlconadaLaura Muinelo-RomayMaría VieitoAlicia AbaloMarta Alonso-NoceloSonia CandamioElena GallardoBeatriz FernándezIhab AbdulkaderMaría de Los Ángeles CasaresAntonio Gómez-TatoRafael López-LópezMiguel AbalPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e40476 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jorge Barbazán Lorena Alonso-Alconada Laura Muinelo-Romay María Vieito Alicia Abalo Marta Alonso-Nocelo Sonia Candamio Elena Gallardo Beatriz Fernández Ihab Abdulkader María de Los Ángeles Casares Antonio Gómez-Tato Rafael López-López Miguel Abal Molecular characterization of circulating tumor cells in human metastatic colorectal cancer.
description	Metastatic colorectal cancer (mCRC) relies on the detachment of aggressive malignant cells from the primary tumor into the bloodstream and, concordantly, the presence of these Circulating Tumor Cells (CTC) is associated with a poor prognosis. In this work, the molecular characterization of CTC from mCRC patients was approached, with the aim of understanding their biology and improving their clinical utility in the management of colorectal cancer patients. For this, EpCAM-based immunoisolation of CTC was combined with whole transcriptome amplification and hybridization onto cDNA microarrays. Gene expression data from mCRC patients, once the background of unspecific immunoisolation from a group of controls had been subtracted, resulted in 410 genes that characterized the CTC population. Bioinformatics were used for the biological interpretation of the data, revealing that CTC are characterized by genes related to cell movement and adhesion, cell death and proliferation, and cell signalling and interaction. RTqPCR on an independent series of mCRC patients and controls was used for the validation of a number of genes related to the main cellular functions characterizing the CTC population. Comparison between primary carcinomas and lung and liver metastases further involved the CTC-genes in the promotion of metastasis. Moreover, the correlation of CTC-gene expression with clinical parameters demonstrated detection and prognosis significance. In conclusion, the molecular characterization of CTC from mCRC patients and the identification of diagnostic and prognostic biomarkers represent an innovative and promising approach in the clinical management of this type of patients.
format	article
author	Jorge Barbazán Lorena Alonso-Alconada Laura Muinelo-Romay María Vieito Alicia Abalo Marta Alonso-Nocelo Sonia Candamio Elena Gallardo Beatriz Fernández Ihab Abdulkader María de Los Ángeles Casares Antonio Gómez-Tato Rafael López-López Miguel Abal
author_facet	Jorge Barbazán Lorena Alonso-Alconada Laura Muinelo-Romay María Vieito Alicia Abalo Marta Alonso-Nocelo Sonia Candamio Elena Gallardo Beatriz Fernández Ihab Abdulkader María de Los Ángeles Casares Antonio Gómez-Tato Rafael López-López Miguel Abal
author_sort	Jorge Barbazán
title	Molecular characterization of circulating tumor cells in human metastatic colorectal cancer.
title_short	Molecular characterization of circulating tumor cells in human metastatic colorectal cancer.
title_full	Molecular characterization of circulating tumor cells in human metastatic colorectal cancer.
title_fullStr	Molecular characterization of circulating tumor cells in human metastatic colorectal cancer.
title_full_unstemmed	Molecular characterization of circulating tumor cells in human metastatic colorectal cancer.
title_sort	molecular characterization of circulating tumor cells in human metastatic colorectal cancer.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/28b3f3215fb94b26a6060573ce85ac50
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Molecular characterization of circulating tumor cells in human metastatic colorectal cancer.

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