miR-148-3p inhibits gastric cancer cell malignant phenotypes and chemotherapy resistance by targeting Bcl2

Gastric cancer(GC) is the fourth most common cancer in the world. This work was designed to explore the biological effects of miR-148-3p on GC. Quantitative reverse transcription-polymerase chain reaction (RT-qPCR) was utilized to analyze the mRNA expression of miR-148-3p in GC cell lines. The mimic...

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Autores principales:	Hongyan Zhang, Feng Liang, Fei Wang, Qianru Xu, Yuxuan Qiu, Xin Lu, Lin Jiang, Kaiyu Jian
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	mir-148-3p bcl2 cell proliferation and invasion gastric cancer chemotherapy resistance Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/28bc2d0d32b3485ea0aba40533ff38f0
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id	oai:doaj.org-article:28bc2d0d32b3485ea0aba40533ff38f0
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spelling	oai:doaj.org-article:28bc2d0d32b3485ea0aba40533ff38f02021-11-17T14:21:59ZmiR-148-3p inhibits gastric cancer cell malignant phenotypes and chemotherapy resistance by targeting Bcl22165-59792165-598710.1080/21655979.2021.2005742https://doaj.org/article/28bc2d0d32b3485ea0aba40533ff38f02021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2005742https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Gastric cancer(GC) is the fourth most common cancer in the world. This work was designed to explore the biological effects of miR-148-3p on GC. Quantitative reverse transcription-polymerase chain reaction (RT-qPCR) was utilized to analyze the mRNA expression of miR-148-3p in GC cell lines. The mimics and inhibitors of miR-148-3p, was carefully transfected into GC cells to up-regulate or down-regulate miR-148-3p expression. Observe the effect on miR-148-3p expression change to GC cell proliferation, colony formation, tumorigenesis, chemotherapy sensitivity, trans-well migration and invasion. Use online database tool to predict the miR-148-3p promising targets, and be verified via RT-qPCR, Western blot and luciferase report. We found that miR-148-3p expression level in GC cells was markedly down-regulated (P <0.05), as compared with human normal gastric mucosal cells GES-1. Otherwise, miR-148-3p overexpression could effectively inhibit the cell proliferation, cell cycle progress, colony formation, anti-apoptosis, anti-migration and anti-invasion in gastric cancer cells, whereas miR-148-3p inhibition exhibited the opposite phenomenon (P<0.05). Further research revealed that Bcl2 set as a direct downstream target of miR-148-3p. Our study firstly confirmed that, miR-148-3p might play a crucial role in tumorigenesis, as well as development of gastric cancer by targeting Bcl2, and could become a promising target for gastric cancer treatment.Hongyan ZhangFeng LiangFei WangQianru XuYuxuan QiuXin LuLin JiangKaiyu JianTaylor & Francis Grouparticlemir-148-3pbcl2cell proliferation and invasiongastric cancerchemotherapy resistanceBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mir-148-3p bcl2 cell proliferation and invasion gastric cancer chemotherapy resistance Biotechnology TP248.13-248.65
spellingShingle	mir-148-3p bcl2 cell proliferation and invasion gastric cancer chemotherapy resistance Biotechnology TP248.13-248.65 Hongyan Zhang Feng Liang Fei Wang Qianru Xu Yuxuan Qiu Xin Lu Lin Jiang Kaiyu Jian miR-148-3p inhibits gastric cancer cell malignant phenotypes and chemotherapy resistance by targeting Bcl2
description	Gastric cancer(GC) is the fourth most common cancer in the world. This work was designed to explore the biological effects of miR-148-3p on GC. Quantitative reverse transcription-polymerase chain reaction (RT-qPCR) was utilized to analyze the mRNA expression of miR-148-3p in GC cell lines. The mimics and inhibitors of miR-148-3p, was carefully transfected into GC cells to up-regulate or down-regulate miR-148-3p expression. Observe the effect on miR-148-3p expression change to GC cell proliferation, colony formation, tumorigenesis, chemotherapy sensitivity, trans-well migration and invasion. Use online database tool to predict the miR-148-3p promising targets, and be verified via RT-qPCR, Western blot and luciferase report. We found that miR-148-3p expression level in GC cells was markedly down-regulated (P <0.05), as compared with human normal gastric mucosal cells GES-1. Otherwise, miR-148-3p overexpression could effectively inhibit the cell proliferation, cell cycle progress, colony formation, anti-apoptosis, anti-migration and anti-invasion in gastric cancer cells, whereas miR-148-3p inhibition exhibited the opposite phenomenon (P<0.05). Further research revealed that Bcl2 set as a direct downstream target of miR-148-3p. Our study firstly confirmed that, miR-148-3p might play a crucial role in tumorigenesis, as well as development of gastric cancer by targeting Bcl2, and could become a promising target for gastric cancer treatment.
format	article
author	Hongyan Zhang Feng Liang Fei Wang Qianru Xu Yuxuan Qiu Xin Lu Lin Jiang Kaiyu Jian
author_facet	Hongyan Zhang Feng Liang Fei Wang Qianru Xu Yuxuan Qiu Xin Lu Lin Jiang Kaiyu Jian
author_sort	Hongyan Zhang
title	miR-148-3p inhibits gastric cancer cell malignant phenotypes and chemotherapy resistance by targeting Bcl2
title_short	miR-148-3p inhibits gastric cancer cell malignant phenotypes and chemotherapy resistance by targeting Bcl2
title_full	miR-148-3p inhibits gastric cancer cell malignant phenotypes and chemotherapy resistance by targeting Bcl2
title_fullStr	miR-148-3p inhibits gastric cancer cell malignant phenotypes and chemotherapy resistance by targeting Bcl2
title_full_unstemmed	miR-148-3p inhibits gastric cancer cell malignant phenotypes and chemotherapy resistance by targeting Bcl2
title_sort	mir-148-3p inhibits gastric cancer cell malignant phenotypes and chemotherapy resistance by targeting bcl2
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/28bc2d0d32b3485ea0aba40533ff38f0
work_keys_str_mv	AT hongyanzhang mir1483pinhibitsgastriccancercellmalignantphenotypesandchemotherapyresistancebytargetingbcl2 AT fengliang mir1483pinhibitsgastriccancercellmalignantphenotypesandchemotherapyresistancebytargetingbcl2 AT feiwang mir1483pinhibitsgastriccancercellmalignantphenotypesandchemotherapyresistancebytargetingbcl2 AT qianruxu mir1483pinhibitsgastriccancercellmalignantphenotypesandchemotherapyresistancebytargetingbcl2 AT yuxuanqiu mir1483pinhibitsgastriccancercellmalignantphenotypesandchemotherapyresistancebytargetingbcl2 AT xinlu mir1483pinhibitsgastriccancercellmalignantphenotypesandchemotherapyresistancebytargetingbcl2 AT linjiang mir1483pinhibitsgastriccancercellmalignantphenotypesandchemotherapyresistancebytargetingbcl2 AT kaiyujian mir1483pinhibitsgastriccancercellmalignantphenotypesandchemotherapyresistancebytargetingbcl2
_version_	1718425401744687104

miR-148-3p inhibits gastric cancer cell malignant phenotypes and chemotherapy resistance by targeting Bcl2

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