Mass Spectrometry-Based Metabolomic and Lipidomic Analysis of the Effect of High Fat/High Sugar Diet and Greenshell<sup>TM</sup> Mussel Feeding on Plasma of Ovariectomized Rats
This study aimed to examine the changes in lipid and metabolite profiles of ovariectomized (OVX) rats with diet-induced metabolic syndrome-associated osteoarthritis (MetOA) after supplementation with greenshell mussel (GSM) using an untargeted liquid chromatography-mass spectrometry (LC-MS) metabolo...
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MDPI AG
2021
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oai:doaj.org-article:28c50b0889ea48dfb71e25cfc1b237732021-11-25T18:20:40ZMass Spectrometry-Based Metabolomic and Lipidomic Analysis of the Effect of High Fat/High Sugar Diet and Greenshell<sup>TM</sup> Mussel Feeding on Plasma of Ovariectomized Rats10.3390/metabo111107542218-1989https://doaj.org/article/28c50b0889ea48dfb71e25cfc1b237732021-10-01T00:00:00Zhttps://www.mdpi.com/2218-1989/11/11/754https://doaj.org/toc/2218-1989This study aimed to examine the changes in lipid and metabolite profiles of ovariectomized (OVX) rats with diet-induced metabolic syndrome-associated osteoarthritis (MetOA) after supplementation with greenshell mussel (GSM) using an untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics approach. Ninety-six rats were fed with one of four diets: control, control supplemented with GSM + GSM, high fat/high sugar (HFHS), or high fat/high sugar enriched with GSM (HFHS + GSM). After 8 weeks on experimental diets, half of the rats in each group underwent OVX and the other half were sham operated. After being fed for an additional 28 weeks, blood samples were collected for the metabolomics analysis. Lipid and polar metabolites were extracted from plasma and analysed by LC-MS. We identified 29 lipid species from four lipid subclasses (phosphatidylcholine, lysophosphatidylcholine, diacylglycerol, and triacylglycerol) and a set of eight metabolites involved in amino acid metabolism (serine, threonine, lysine, valine, histidine, pipecolic acid, 3-methylcytidine, and cholic acid) as potential biomarkers for the effect of HFHS diet and GSM supplementation. GSM incorporation more specifically in the control diet generated significant alterations in the levels of several lipids and metabolites. Further studies are required to validate these findings that identify potential biomarkers to follow OA progression and to monitor the impact of GSM supplementation.Maryam AbshiriniDiana CabreraKarl FraserParkpoom SiriarchavatanaFrances M. WolberMatthew R. MillerHong Sabrina TianMarlena C. KrugerMDPI AGarticleosteoarthritismetabolic dysregulationdiet-induced obese ratsmetabolomicslipidomicMicrobiologyQR1-502ENMetabolites, Vol 11, Iss 754, p 754 (2021) |
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| collection |
DOAJ |
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| topic |
osteoarthritis metabolic dysregulation diet-induced obese rats metabolomics lipidomic Microbiology QR1-502 |
| spellingShingle |
osteoarthritis metabolic dysregulation diet-induced obese rats metabolomics lipidomic Microbiology QR1-502 Maryam Abshirini Diana Cabrera Karl Fraser Parkpoom Siriarchavatana Frances M. Wolber Matthew R. Miller Hong Sabrina Tian Marlena C. Kruger Mass Spectrometry-Based Metabolomic and Lipidomic Analysis of the Effect of High Fat/High Sugar Diet and Greenshell<sup>TM</sup> Mussel Feeding on Plasma of Ovariectomized Rats |
| description |
This study aimed to examine the changes in lipid and metabolite profiles of ovariectomized (OVX) rats with diet-induced metabolic syndrome-associated osteoarthritis (MetOA) after supplementation with greenshell mussel (GSM) using an untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics approach. Ninety-six rats were fed with one of four diets: control, control supplemented with GSM + GSM, high fat/high sugar (HFHS), or high fat/high sugar enriched with GSM (HFHS + GSM). After 8 weeks on experimental diets, half of the rats in each group underwent OVX and the other half were sham operated. After being fed for an additional 28 weeks, blood samples were collected for the metabolomics analysis. Lipid and polar metabolites were extracted from plasma and analysed by LC-MS. We identified 29 lipid species from four lipid subclasses (phosphatidylcholine, lysophosphatidylcholine, diacylglycerol, and triacylglycerol) and a set of eight metabolites involved in amino acid metabolism (serine, threonine, lysine, valine, histidine, pipecolic acid, 3-methylcytidine, and cholic acid) as potential biomarkers for the effect of HFHS diet and GSM supplementation. GSM incorporation more specifically in the control diet generated significant alterations in the levels of several lipids and metabolites. Further studies are required to validate these findings that identify potential biomarkers to follow OA progression and to monitor the impact of GSM supplementation. |
| format |
article |
| author |
Maryam Abshirini Diana Cabrera Karl Fraser Parkpoom Siriarchavatana Frances M. Wolber Matthew R. Miller Hong Sabrina Tian Marlena C. Kruger |
| author_facet |
Maryam Abshirini Diana Cabrera Karl Fraser Parkpoom Siriarchavatana Frances M. Wolber Matthew R. Miller Hong Sabrina Tian Marlena C. Kruger |
| author_sort |
Maryam Abshirini |
| title |
Mass Spectrometry-Based Metabolomic and Lipidomic Analysis of the Effect of High Fat/High Sugar Diet and Greenshell<sup>TM</sup> Mussel Feeding on Plasma of Ovariectomized Rats |
| title_short |
Mass Spectrometry-Based Metabolomic and Lipidomic Analysis of the Effect of High Fat/High Sugar Diet and Greenshell<sup>TM</sup> Mussel Feeding on Plasma of Ovariectomized Rats |
| title_full |
Mass Spectrometry-Based Metabolomic and Lipidomic Analysis of the Effect of High Fat/High Sugar Diet and Greenshell<sup>TM</sup> Mussel Feeding on Plasma of Ovariectomized Rats |
| title_fullStr |
Mass Spectrometry-Based Metabolomic and Lipidomic Analysis of the Effect of High Fat/High Sugar Diet and Greenshell<sup>TM</sup> Mussel Feeding on Plasma of Ovariectomized Rats |
| title_full_unstemmed |
Mass Spectrometry-Based Metabolomic and Lipidomic Analysis of the Effect of High Fat/High Sugar Diet and Greenshell<sup>TM</sup> Mussel Feeding on Plasma of Ovariectomized Rats |
| title_sort |
mass spectrometry-based metabolomic and lipidomic analysis of the effect of high fat/high sugar diet and greenshell<sup>tm</sup> mussel feeding on plasma of ovariectomized rats |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/28c50b0889ea48dfb71e25cfc1b23773 |
| work_keys_str_mv |
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