Galleria mellonella (greater wax moth) as an eco-friendly in vivo approach for the assessment of the acute toxicity of medicinal plants: Application to some plants from Cameroon

Background: The evaluation of medicinal plants’ toxicity is a prerequisite prior to their usage. The vertebrate models used for this purpose are often the object of ethical consideration. Though invertebrate models including Galleria mellonella (GM) have demonstrated the ability to be used to assess...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mbarga Manga Joseph Arsene, Podoprigora Irina Viktorovna, Anyutoulou Kitio Linda Davares
Formato:	article
Lenguaje:	EN
Publicado:	Tripoli University 2021
Materias:	medicinal plants greater wax moth galleria mellonella insect model toxicity Zoology QL1-991
Acceso en línea:	https://doaj.org/article/28c6f9e877ad4b1b8d580e9ec3a6415b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:28c6f9e877ad4b1b8d580e9ec3a6415b
record_format	dspace
spelling	oai:doaj.org-article:28c6f9e877ad4b1b8d580e9ec3a6415b2021-11-21T07:25:40ZGalleria mellonella (greater wax moth) as an eco-friendly in vivo approach for the assessment of the acute toxicity of medicinal plants: Application to some plants from Cameroon10.5455/OVJ.2021.v11.i4.152226-44852218-6050https://doaj.org/article/28c6f9e877ad4b1b8d580e9ec3a6415b2021-11-01T00:00:00Zhttps://www.openveterinaryjournal.com/OVJ-2021-07-139%20M.M.J.%20Arsene%20et%20al.pdfhttps://doaj.org/toc/2226-4485https://doaj.org/toc/2218-6050Background: The evaluation of medicinal plants’ toxicity is a prerequisite prior to their usage. The vertebrate models used for this purpose are often the object of ethical consideration. Though invertebrate models including Galleria mellonella (GM) have demonstrated the ability to be used to assess the toxicity of various products. To our knowledge, GM has never been exploited to determine the toxicity of medicinal plants. Aim: The aim of this study was to demonstrate the potential of GM larvae as a simple, inexpensive and rapid model for the evaluation of the toxicity of herbal medicines. Method: In this study, the toxicity of aqueous and ethanolic (80%, v/v) extracts of seven (7) well known plants from Cameroon namely leaves of Cymbopogon citratus (DC.) Stapf, Moringa oleifera Lam and Vernonia amygdalina Delile; barks of Cinchona officinalis and Enantia chlorantha Oliv; barks and seeds of Garcinia lucida Vesque and leaves and seeds of Azadirachta indica (Neem) was evaluated using the larval form of GM. The median lethal doses (LD50), 90% lethal doses (LD90) and 100% lethal doses (LD100) were determined using the spline cubic survival curves and equations from the data obtained on the survival rate of G. mellonella 24h after the injection with the extracts. Results: We found that distilled water extracted a more important mass of phytochemical compounds (7.4-21.2%) compared to ethanolic solution (5.8-12.4%). Lethal doses varied depending on the plant materials and ethanolic extracts (HAE) were more toxic to GM than aqueous ones. The LD50 (mg/ml) of the tested extracts varied from 4.87 (3.90 g/kg bw (body weight)) to >200 (> 166.67 g/kg bw), the LD90 (mg/ml) from 25.00 (18.52 g/kg bw) to >200 (> 181.82 g/kg bw) and LD100 (mg/ml) from 45.00 (40.91 g/kg bw) to > 200 (>181.82 g/kg bw). The HAE of A. indica seed and C. officinalis bark exhibited the highest toxicity with LD50 (g/kg bw) of 3.90 and 4.81 respectively. Conclusion: The results obtained in this study suggest that G. mellonella can be used as a sensitive, reliable, and robust eco-friendly model to gauge the toxicity of medicinal plants. Thus, avoid the sacrifice of vertebrate models often used for this purpose to limit ethical concerns.Mbarga Manga Joseph ArsenePodoprigora Irina ViktorovnaAnyutoulou Kitio Linda DavaresTripoli Universityarticlemedicinal plantsgreater wax mothgalleria mellonellainsect modeltoxicityZoologyQL1-991ENOpen Veterinary Journal, Vol 11, Iss 4, Pp 651-661 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	medicinal plants greater wax moth galleria mellonella insect model toxicity Zoology QL1-991
spellingShingle	medicinal plants greater wax moth galleria mellonella insect model toxicity Zoology QL1-991 Mbarga Manga Joseph Arsene Podoprigora Irina Viktorovna Anyutoulou Kitio Linda Davares Galleria mellonella (greater wax moth) as an eco-friendly in vivo approach for the assessment of the acute toxicity of medicinal plants: Application to some plants from Cameroon
description	Background: The evaluation of medicinal plants’ toxicity is a prerequisite prior to their usage. The vertebrate models used for this purpose are often the object of ethical consideration. Though invertebrate models including Galleria mellonella (GM) have demonstrated the ability to be used to assess the toxicity of various products. To our knowledge, GM has never been exploited to determine the toxicity of medicinal plants. Aim: The aim of this study was to demonstrate the potential of GM larvae as a simple, inexpensive and rapid model for the evaluation of the toxicity of herbal medicines. Method: In this study, the toxicity of aqueous and ethanolic (80%, v/v) extracts of seven (7) well known plants from Cameroon namely leaves of Cymbopogon citratus (DC.) Stapf, Moringa oleifera Lam and Vernonia amygdalina Delile; barks of Cinchona officinalis and Enantia chlorantha Oliv; barks and seeds of Garcinia lucida Vesque and leaves and seeds of Azadirachta indica (Neem) was evaluated using the larval form of GM. The median lethal doses (LD50), 90% lethal doses (LD90) and 100% lethal doses (LD100) were determined using the spline cubic survival curves and equations from the data obtained on the survival rate of G. mellonella 24h after the injection with the extracts. Results: We found that distilled water extracted a more important mass of phytochemical compounds (7.4-21.2%) compared to ethanolic solution (5.8-12.4%). Lethal doses varied depending on the plant materials and ethanolic extracts (HAE) were more toxic to GM than aqueous ones. The LD50 (mg/ml) of the tested extracts varied from 4.87 (3.90 g/kg bw (body weight)) to >200 (> 166.67 g/kg bw), the LD90 (mg/ml) from 25.00 (18.52 g/kg bw) to >200 (> 181.82 g/kg bw) and LD100 (mg/ml) from 45.00 (40.91 g/kg bw) to > 200 (>181.82 g/kg bw). The HAE of A. indica seed and C. officinalis bark exhibited the highest toxicity with LD50 (g/kg bw) of 3.90 and 4.81 respectively. Conclusion: The results obtained in this study suggest that G. mellonella can be used as a sensitive, reliable, and robust eco-friendly model to gauge the toxicity of medicinal plants. Thus, avoid the sacrifice of vertebrate models often used for this purpose to limit ethical concerns.
format	article
author	Mbarga Manga Joseph Arsene Podoprigora Irina Viktorovna Anyutoulou Kitio Linda Davares
author_facet	Mbarga Manga Joseph Arsene Podoprigora Irina Viktorovna Anyutoulou Kitio Linda Davares
author_sort	Mbarga Manga Joseph Arsene
title	Galleria mellonella (greater wax moth) as an eco-friendly in vivo approach for the assessment of the acute toxicity of medicinal plants: Application to some plants from Cameroon
title_short	Galleria mellonella (greater wax moth) as an eco-friendly in vivo approach for the assessment of the acute toxicity of medicinal plants: Application to some plants from Cameroon
title_full	Galleria mellonella (greater wax moth) as an eco-friendly in vivo approach for the assessment of the acute toxicity of medicinal plants: Application to some plants from Cameroon
title_fullStr	Galleria mellonella (greater wax moth) as an eco-friendly in vivo approach for the assessment of the acute toxicity of medicinal plants: Application to some plants from Cameroon
title_full_unstemmed	Galleria mellonella (greater wax moth) as an eco-friendly in vivo approach for the assessment of the acute toxicity of medicinal plants: Application to some plants from Cameroon
title_sort	galleria mellonella (greater wax moth) as an eco-friendly in vivo approach for the assessment of the acute toxicity of medicinal plants: application to some plants from cameroon
publisher	Tripoli University
publishDate	2021
url	https://doaj.org/article/28c6f9e877ad4b1b8d580e9ec3a6415b
work_keys_str_mv	AT mbargamangajosepharsene galleriamellonellagreaterwaxmothasanecofriendlyinvivoapproachfortheassessmentoftheacutetoxicityofmedicinalplantsapplicationtosomeplantsfromcameroon AT podoprigorairinaviktorovna galleriamellonellagreaterwaxmothasanecofriendlyinvivoapproachfortheassessmentoftheacutetoxicityofmedicinalplantsapplicationtosomeplantsfromcameroon AT anyutouloukitiolindadavares galleriamellonellagreaterwaxmothasanecofriendlyinvivoapproachfortheassessmentoftheacutetoxicityofmedicinalplantsapplicationtosomeplantsfromcameroon
_version_	1718419353709314048

Galleria mellonella (greater wax moth) as an eco-friendly in vivo approach for the assessment of the acute toxicity of medicinal plants: Application to some plants from Cameroon

Ejemplares similares