Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo

Optimization of the recently discovered Class 2 CRISPR protein Cpf1 has the potential to promote its applications in gene editing and therapeutics. Here, the authors find that extending the 5′ end of the crRNA can increase both the editing efficiency and delivery of Cpf1 in vitro and in vivo.

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Autores principales:	Hyo Min Park, Hui Liu, Joann Wu, Anthony Chong, Vanessa Mackley, Christof Fellmann, Anirudh Rao, Fuguo Jiang, Hunghao Chu, Niren Murthy, Kunwoo Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/28c8eaacf2fc48ad8caaf32212f85d51
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spelling	oai:doaj.org-article:28c8eaacf2fc48ad8caaf32212f85d512021-12-02T16:49:19ZExtension of the crRNA enhances Cpf1 gene editing in vitro and in vivo10.1038/s41467-018-05641-32041-1723https://doaj.org/article/28c8eaacf2fc48ad8caaf32212f85d512018-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05641-3https://doaj.org/toc/2041-1723Optimization of the recently discovered Class 2 CRISPR protein Cpf1 has the potential to promote its applications in gene editing and therapeutics. Here, the authors find that extending the 5′ end of the crRNA can increase both the editing efficiency and delivery of Cpf1 in vitro and in vivo.Hyo Min ParkHui LiuJoann WuAnthony ChongVanessa MackleyChristof FellmannAnirudh RaoFuguo JiangHunghao ChuNiren MurthyKunwoo LeeNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Hyo Min Park Hui Liu Joann Wu Anthony Chong Vanessa Mackley Christof Fellmann Anirudh Rao Fuguo Jiang Hunghao Chu Niren Murthy Kunwoo Lee Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo
description	Optimization of the recently discovered Class 2 CRISPR protein Cpf1 has the potential to promote its applications in gene editing and therapeutics. Here, the authors find that extending the 5′ end of the crRNA can increase both the editing efficiency and delivery of Cpf1 in vitro and in vivo.
format	article
author	Hyo Min Park Hui Liu Joann Wu Anthony Chong Vanessa Mackley Christof Fellmann Anirudh Rao Fuguo Jiang Hunghao Chu Niren Murthy Kunwoo Lee
author_facet	Hyo Min Park Hui Liu Joann Wu Anthony Chong Vanessa Mackley Christof Fellmann Anirudh Rao Fuguo Jiang Hunghao Chu Niren Murthy Kunwoo Lee
author_sort	Hyo Min Park
title	Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo
title_short	Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo
title_full	Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo
title_fullStr	Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo
title_full_unstemmed	Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo
title_sort	extension of the crrna enhances cpf1 gene editing in vitro and in vivo
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/28c8eaacf2fc48ad8caaf32212f85d51
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Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo

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