Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo

Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo

Optimization of the recently discovered Class 2 CRISPR protein Cpf1 has the potential to promote its applications in gene editing and therapeutics. Here, the authors find that extending the 5′ end of the crRNA can increase both the editing efficiency and delivery of Cpf1 in vitro and in vivo.

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Autores principales: Hyo Min Park, Hui Liu, Joann Wu, Anthony Chong, Vanessa Mackley, Christof Fellmann, Anirudh Rao, Fuguo Jiang, Hunghao Chu, Niren Murthy, Kunwoo Lee
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
Materias:
Science
Q
Acceso en línea:https://doaj.org/article/28c8eaacf2fc48ad8caaf32212f85d51
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