Objective Short Sleep Duration is Related to the Peripheral Inflammasome Dysregulation in Patients with Chronic Insomnia

Jihui wang, Xiaoli Wu, Wenjing Liang, Minhua chen, Chongbang Zhao, Xianglan Wang Department of Psychiatry, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, People’s Republic of ChinaCorrespondence: Xianglan WangDepartment of Psychiatry, The Third Affiliated Hospital...

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Autores principales:	Wang J, Wu X, Liang W, Chen M, Zhao C, Wang X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	insomnia short sleep duration polysomnography fragmentation inflammation nlrp3 Psychiatry RC435-571 Neurophysiology and neuropsychology QP351-495
Acceso en línea:	https://doaj.org/article/28d2694b2b194fb99e11ff31d6184f2d
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spelling	oai:doaj.org-article:28d2694b2b194fb99e11ff31d6184f2d2021-12-02T12:31:52ZObjective Short Sleep Duration is Related to the Peripheral Inflammasome Dysregulation in Patients with Chronic Insomnia1179-1608https://doaj.org/article/28d2694b2b194fb99e11ff31d6184f2d2020-10-01T00:00:00Zhttps://test.dovepress.com/objective-short-sleep-duration-is-related-to-the-peripheral-inflammaso-peer-reviewed-article-NSShttps://doaj.org/toc/1179-1608Jihui wang, Xiaoli Wu, Wenjing Liang, Minhua chen, Chongbang Zhao, Xianglan Wang Department of Psychiatry, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, People’s Republic of ChinaCorrespondence: Xianglan WangDepartment of Psychiatry, The Third Affiliated Hospital, Sun Yat-Sen University, No. 60, Tianhe Road, Tianhe District, Guangzhou 510630, Guangdong, People’s Republic of ChinaTel +8602085253129Email wxiangl@mail.sysu.edu.cnObjective: Insomnia with objective short sleep duration (IOSSD) is associated with an increased risk of cardiovascular morbidity, diabetes, neurocognitive impairment, and mortality. Inflammation is believed to be one of the main links between IOSSD and these diseases. The role of nucleotide-binding domain and leucine-rich repeat protein-3 (NLRP3) inflammasome in inducing activation of inflammatory signaling in IOSSD is not clear. In this study, we investigated the expression of NLRP3 inflammasome in patients with IOSSD to clarify this issue.Methods: Thirty-six patients with insomnia and 20 age- and sex-matched healthy controls were sequentially recruited. Subjects were categorized into three groups: IOSSD (sleep duration < 6h, n=20), insomnia with objective normal sleep duration (IONSD, sleep duration ≥ 6h, n=16) and healthy controls (n=20). Objective sleep parameters were measured by overnight polysomnography. Peripheral NLRP3 inflammasome protein levels [NLRP3, apoptosis-associated speck-like protein containing a CARD (ASC), caspase l] and cytokines [interleukin (IL)-1β and IL-18] were assessed by Western blotting and ELISA, respectively.Results: IOSSD group showed significantly increased protein expressions of ASC and caspase-1 compared to IONSD and healthy controls and significantly increased IL-18 levels compared to healthy controls. On correlation analysis, total sleep time showed an inverse correlation with NLRP3, ASC, IL-18, and IL-1β levels. Wake after sleep onset (WASO) showed a positive correlation with NLRP3, ASC, caspase-1, and IL-1β levels. N3 sleep ratio showed a significant negative correlation with NLRP3, ASC, and IL-18 levels.Conclusion: The current study demonstrated upregulation of NLRP3 inflammasome in IOSSD. Short sleep duration, decreased slow wave sleep, and sleep fragmentation may contribute to dysregulation of NLRP3 inflammasome.Keywords: insomnia, short sleep duration, polysomnography, fragmentation, inflammation, NLRP3Wang JWu XLiang WChen MZhao CWang XDove Medical Pressarticleinsomniashort sleep durationpolysomnographyfragmentationinflammationnlrp3PsychiatryRC435-571Neurophysiology and neuropsychologyQP351-495ENNature and Science of Sleep, Vol Volume 12, Pp 759-766 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	insomnia short sleep duration polysomnography fragmentation inflammation nlrp3 Psychiatry RC435-571 Neurophysiology and neuropsychology QP351-495
spellingShingle	insomnia short sleep duration polysomnography fragmentation inflammation nlrp3 Psychiatry RC435-571 Neurophysiology and neuropsychology QP351-495 Wang J Wu X Liang W Chen M Zhao C Wang X Objective Short Sleep Duration is Related to the Peripheral Inflammasome Dysregulation in Patients with Chronic Insomnia
description	Jihui wang, Xiaoli Wu, Wenjing Liang, Minhua chen, Chongbang Zhao, Xianglan Wang Department of Psychiatry, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, People’s Republic of ChinaCorrespondence: Xianglan WangDepartment of Psychiatry, The Third Affiliated Hospital, Sun Yat-Sen University, No. 60, Tianhe Road, Tianhe District, Guangzhou 510630, Guangdong, People’s Republic of ChinaTel +8602085253129Email wxiangl@mail.sysu.edu.cnObjective: Insomnia with objective short sleep duration (IOSSD) is associated with an increased risk of cardiovascular morbidity, diabetes, neurocognitive impairment, and mortality. Inflammation is believed to be one of the main links between IOSSD and these diseases. The role of nucleotide-binding domain and leucine-rich repeat protein-3 (NLRP3) inflammasome in inducing activation of inflammatory signaling in IOSSD is not clear. In this study, we investigated the expression of NLRP3 inflammasome in patients with IOSSD to clarify this issue.Methods: Thirty-six patients with insomnia and 20 age- and sex-matched healthy controls were sequentially recruited. Subjects were categorized into three groups: IOSSD (sleep duration < 6h, n=20), insomnia with objective normal sleep duration (IONSD, sleep duration ≥ 6h, n=16) and healthy controls (n=20). Objective sleep parameters were measured by overnight polysomnography. Peripheral NLRP3 inflammasome protein levels [NLRP3, apoptosis-associated speck-like protein containing a CARD (ASC), caspase l] and cytokines [interleukin (IL)-1β and IL-18] were assessed by Western blotting and ELISA, respectively.Results: IOSSD group showed significantly increased protein expressions of ASC and caspase-1 compared to IONSD and healthy controls and significantly increased IL-18 levels compared to healthy controls. On correlation analysis, total sleep time showed an inverse correlation with NLRP3, ASC, IL-18, and IL-1β levels. Wake after sleep onset (WASO) showed a positive correlation with NLRP3, ASC, caspase-1, and IL-1β levels. N3 sleep ratio showed a significant negative correlation with NLRP3, ASC, and IL-18 levels.Conclusion: The current study demonstrated upregulation of NLRP3 inflammasome in IOSSD. Short sleep duration, decreased slow wave sleep, and sleep fragmentation may contribute to dysregulation of NLRP3 inflammasome.Keywords: insomnia, short sleep duration, polysomnography, fragmentation, inflammation, NLRP3
format	article
author	Wang J Wu X Liang W Chen M Zhao C Wang X
author_facet	Wang J Wu X Liang W Chen M Zhao C Wang X
author_sort	Wang J
title	Objective Short Sleep Duration is Related to the Peripheral Inflammasome Dysregulation in Patients with Chronic Insomnia
title_short	Objective Short Sleep Duration is Related to the Peripheral Inflammasome Dysregulation in Patients with Chronic Insomnia
title_full	Objective Short Sleep Duration is Related to the Peripheral Inflammasome Dysregulation in Patients with Chronic Insomnia
title_fullStr	Objective Short Sleep Duration is Related to the Peripheral Inflammasome Dysregulation in Patients with Chronic Insomnia
title_full_unstemmed	Objective Short Sleep Duration is Related to the Peripheral Inflammasome Dysregulation in Patients with Chronic Insomnia
title_sort	objective short sleep duration is related to the peripheral inflammasome dysregulation in patients with chronic insomnia
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/28d2694b2b194fb99e11ff31d6184f2d
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Objective Short Sleep Duration is Related to the Peripheral Inflammasome Dysregulation in Patients with Chronic Insomnia

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