Isolation, identification and characterization of an electrogenic microalgae strain.

Extracellular electron transfer involving microbes is important as it closely reflects the ability of cells to communicate with the environment. However, there are few reports on electron transfer mechanisms of pure microalgae and a lack of any model alga to study the transfer processes. In the pres...

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Autores principales:	Yicheng Wu, Kai Guan, Zejie Wang, Bing Xu, Feng Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/28d3644ccd8042388b78916dea82c2c1
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spelling	oai:doaj.org-article:28d3644ccd8042388b78916dea82c2c12021-11-18T08:57:11ZIsolation, identification and characterization of an electrogenic microalgae strain.1932-620310.1371/journal.pone.0073442https://doaj.org/article/28d3644ccd8042388b78916dea82c2c12013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24019922/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Extracellular electron transfer involving microbes is important as it closely reflects the ability of cells to communicate with the environment. However, there are few reports on electron transfer mechanisms of pure microalgae and a lack of any model alga to study the transfer processes. In the present study, nine green microalgae species were isolated from wastewater and characterized in terms of their ability to transfer electrons between cells and an electrode. One species showed direct electron transfer via membrane-associated proteins and indirect electron transfer via secreted oxygen. The microalga was identified as Desmodesmus sp. based on phylogenetic analysis and electron microscopy. Electrochemical tests demonstrated that Desmodesmus sp. was able to act as a cathodic microorganism. Stable current densities of -0.24, 35.54 and 170 mA m(-2) were achieved at potentials of +0.2, -0.2 and -0.4 V, respectively, under illumination. Dissolved oxygen concentration measurement showed gradients within the microalgae biofilm: 18.3 mg L(-1) in light decreasing to 4.29 mg L(-1) in the dark. This study diversified the exoelectrogen library and provided a potential model microalga to explore the associated mechanism of extracellular electron transfer.Yicheng WuKai GuanZejie WangBing XuFeng ZhaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e73442 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yicheng Wu Kai Guan Zejie Wang Bing Xu Feng Zhao Isolation, identification and characterization of an electrogenic microalgae strain.
description	Extracellular electron transfer involving microbes is important as it closely reflects the ability of cells to communicate with the environment. However, there are few reports on electron transfer mechanisms of pure microalgae and a lack of any model alga to study the transfer processes. In the present study, nine green microalgae species were isolated from wastewater and characterized in terms of their ability to transfer electrons between cells and an electrode. One species showed direct electron transfer via membrane-associated proteins and indirect electron transfer via secreted oxygen. The microalga was identified as Desmodesmus sp. based on phylogenetic analysis and electron microscopy. Electrochemical tests demonstrated that Desmodesmus sp. was able to act as a cathodic microorganism. Stable current densities of -0.24, 35.54 and 170 mA m(-2) were achieved at potentials of +0.2, -0.2 and -0.4 V, respectively, under illumination. Dissolved oxygen concentration measurement showed gradients within the microalgae biofilm: 18.3 mg L(-1) in light decreasing to 4.29 mg L(-1) in the dark. This study diversified the exoelectrogen library and provided a potential model microalga to explore the associated mechanism of extracellular electron transfer.
format	article
author	Yicheng Wu Kai Guan Zejie Wang Bing Xu Feng Zhao
author_facet	Yicheng Wu Kai Guan Zejie Wang Bing Xu Feng Zhao
author_sort	Yicheng Wu
title	Isolation, identification and characterization of an electrogenic microalgae strain.
title_short	Isolation, identification and characterization of an electrogenic microalgae strain.
title_full	Isolation, identification and characterization of an electrogenic microalgae strain.
title_fullStr	Isolation, identification and characterization of an electrogenic microalgae strain.
title_full_unstemmed	Isolation, identification and characterization of an electrogenic microalgae strain.
title_sort	isolation, identification and characterization of an electrogenic microalgae strain.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/28d3644ccd8042388b78916dea82c2c1
work_keys_str_mv	AT yichengwu isolationidentificationandcharacterizationofanelectrogenicmicroalgaestrain AT kaiguan isolationidentificationandcharacterizationofanelectrogenicmicroalgaestrain AT zejiewang isolationidentificationandcharacterizationofanelectrogenicmicroalgaestrain AT bingxu isolationidentificationandcharacterizationofanelectrogenicmicroalgaestrain AT fengzhao isolationidentificationandcharacterizationofanelectrogenicmicroalgaestrain
_version_	1718421070536507392

Isolation, identification and characterization of an electrogenic microalgae strain.

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