MiR-135b-5p promotes viability, proliferation, migration and invasion of gastric cancer cells by targeting Krüppel-like factor 4 (KLF4)

Introduction The expression of MiR-135b-5p was up-regulated while Krüppel-like factor 4 (KLF4) expression was extremely low in human gastric carcinoma (GC) tissues. This study aimed to explore the role of miR-135b-5p in GC cells and its influence on various cell capacity and viability by targeting K...

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Autores principales:	Zhi Chen, Yongjian Gao, Shuohui Gao, Defeng Song, Ye Feng
Formato:	article
Lenguaje:	EN
Publicado:	Termedia Publishing House 2019
Materias:	gastric cancer mir-135b-5p klf4 bgc-823 krüppel-like factor 4 Medicine R
Acceso en línea:	https://doaj.org/article/28dfcc57b49743d3abb86767ba70d15e
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spelling	oai:doaj.org-article:28dfcc57b49743d3abb86767ba70d15e2021-12-02T18:33:05ZMiR-135b-5p promotes viability, proliferation, migration and invasion of gastric cancer cells by targeting Krüppel-like factor 4 (KLF4)1734-19221896-915110.5114/aoms.2019.87761https://doaj.org/article/28dfcc57b49743d3abb86767ba70d15e2019-12-01T00:00:00Zhttps://www.archivesofmedicalscience.com/MiR-135b-5p-promotes-viability-proliferation-migration-and-invasion-of-gastric-cancer,76022,0,2.htmlhttps://doaj.org/toc/1734-1922https://doaj.org/toc/1896-9151Introduction The expression of MiR-135b-5p was up-regulated while Krüppel-like factor 4 (KLF4) expression was extremely low in human gastric carcinoma (GC) tissues. This study aimed to explore the role of miR-135b-5p in GC cells and its influence on various cell capacity and viability by targeting KLF4. Material and methods The dual-luciferase reporter assay was first performed and the target relationship between miR-135b-5p and KLF4 was confirmed. Then three GC cell lines and the human normal gastric epithelial cell line (GES1) were analyzed for the expression level of miR-135b-5p and KLF4 mRNA by RT-qPCR. The BGC-823 GC cell line was chosen for subsequent assays. Results The expression of miR-135b-5p and KLF4 was manipulated via transfection. The changes of proliferation, invasion, migration, viability, cycle and apoptosis of GC cells were evaluated by MTS, colony formation assay, transwell assay, wound healing assay and flow cytometry assay, respectively. Overexpression of MiR-135b-5p enhanced viability, proliferation, invasion and migration of GC cells, increased cell viability and reduced cell apoptosis. Replenishing of KLF4 functioned oppositely. Conclusions The inhibitory effects of ectopic KLF4 could be attenuated by co-transfection of miR-135b-5p. Collective data suggested that miR-135b-5p has a tumor-promoting role in GC cells via downregulating KLF4. Hence, inhibition of miR-135b-5p could be valuable for treatment of gastric cancer.Zhi ChenYongjian GaoShuohui GaoDefeng SongYe FengTermedia Publishing Housearticlegastric cancermir-135b-5pklf4bgc-823krüppel-like factor 4MedicineRENArchives of Medical Science, Vol 16, Iss 1, Pp 167-176 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	gastric cancer mir-135b-5p klf4 bgc-823 krüppel-like factor 4 Medicine R
spellingShingle	gastric cancer mir-135b-5p klf4 bgc-823 krüppel-like factor 4 Medicine R Zhi Chen Yongjian Gao Shuohui Gao Defeng Song Ye Feng MiR-135b-5p promotes viability, proliferation, migration and invasion of gastric cancer cells by targeting Krüppel-like factor 4 (KLF4)
description	Introduction The expression of MiR-135b-5p was up-regulated while Krüppel-like factor 4 (KLF4) expression was extremely low in human gastric carcinoma (GC) tissues. This study aimed to explore the role of miR-135b-5p in GC cells and its influence on various cell capacity and viability by targeting KLF4. Material and methods The dual-luciferase reporter assay was first performed and the target relationship between miR-135b-5p and KLF4 was confirmed. Then three GC cell lines and the human normal gastric epithelial cell line (GES1) were analyzed for the expression level of miR-135b-5p and KLF4 mRNA by RT-qPCR. The BGC-823 GC cell line was chosen for subsequent assays. Results The expression of miR-135b-5p and KLF4 was manipulated via transfection. The changes of proliferation, invasion, migration, viability, cycle and apoptosis of GC cells were evaluated by MTS, colony formation assay, transwell assay, wound healing assay and flow cytometry assay, respectively. Overexpression of MiR-135b-5p enhanced viability, proliferation, invasion and migration of GC cells, increased cell viability and reduced cell apoptosis. Replenishing of KLF4 functioned oppositely. Conclusions The inhibitory effects of ectopic KLF4 could be attenuated by co-transfection of miR-135b-5p. Collective data suggested that miR-135b-5p has a tumor-promoting role in GC cells via downregulating KLF4. Hence, inhibition of miR-135b-5p could be valuable for treatment of gastric cancer.
format	article
author	Zhi Chen Yongjian Gao Shuohui Gao Defeng Song Ye Feng
author_facet	Zhi Chen Yongjian Gao Shuohui Gao Defeng Song Ye Feng
author_sort	Zhi Chen
title	MiR-135b-5p promotes viability, proliferation, migration and invasion of gastric cancer cells by targeting Krüppel-like factor 4 (KLF4)
title_short	MiR-135b-5p promotes viability, proliferation, migration and invasion of gastric cancer cells by targeting Krüppel-like factor 4 (KLF4)
title_full	MiR-135b-5p promotes viability, proliferation, migration and invasion of gastric cancer cells by targeting Krüppel-like factor 4 (KLF4)
title_fullStr	MiR-135b-5p promotes viability, proliferation, migration and invasion of gastric cancer cells by targeting Krüppel-like factor 4 (KLF4)
title_full_unstemmed	MiR-135b-5p promotes viability, proliferation, migration and invasion of gastric cancer cells by targeting Krüppel-like factor 4 (KLF4)
title_sort	mir-135b-5p promotes viability, proliferation, migration and invasion of gastric cancer cells by targeting krüppel-like factor 4 (klf4)
publisher	Termedia Publishing House
publishDate	2019
url	https://doaj.org/article/28dfcc57b49743d3abb86767ba70d15e
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MiR-135b-5p promotes viability, proliferation, migration and invasion of gastric cancer cells by targeting Krüppel-like factor 4 (KLF4)

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