Secretome of in vitro cultured human embryos contains extracellular vesicles that are uptaken by the maternal side

Abstract Communication between embryo and maternal endometrium occurs during a specific time frame in which implantation is possible. Here we demonstrate for the first time that conditioned media from non-manipulated human embryos cultured in vitro for 3 days or up to the blastocyst stage contain ex...

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Autores principales:	Elisa Giacomini, Riccardo Vago, Ana Maria Sanchez, Paola Podini, Natasa Zarovni, Valentina Murdica, Roberta Rizzo, Daria Bortolotti, Massimo Candiani, Paola Viganò
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/28e2c579bfa94dbd808d6db598e2e8c6
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spelling	oai:doaj.org-article:28e2c579bfa94dbd808d6db598e2e8c62021-12-02T12:31:59ZSecretome of in vitro cultured human embryos contains extracellular vesicles that are uptaken by the maternal side10.1038/s41598-017-05549-w2045-2322https://doaj.org/article/28e2c579bfa94dbd808d6db598e2e8c62017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05549-whttps://doaj.org/toc/2045-2322Abstract Communication between embryo and maternal endometrium occurs during a specific time frame in which implantation is possible. Here we demonstrate for the first time that conditioned media from non-manipulated human embryos cultured in vitro for 3 days or up to the blastocyst stage contain extracellular vesicles (EVs) with a diameter of 50 to 200 nm and bearing the traditional microvesicle and exosome marker proteins CD63, CD9 and ALIX. The embryonic origin of these EVs has been confirmed by the presence of stemness gene transcripts and their enrichment in the non-classical HLA-G protein. NANOG and POU5F1 transcripts were shown to be contained in vesicles deriving from embryos at different stages of development. In line with a higher detection rate of the HLA-G protein in blastocysts compared to cleavage stage embryos, a significantly higher amount of HLA-G was found in vesicles accumulated in spent media from day 3 to day 5 of development compared to those isolated from the earlier stage. Uptake of dye-labeled embryo-derived EVs by human primary endometrial epithelial and stromal cells was also demonstrated with a fluorescence intensity signal significantly higher for cells treated with vesicles derived from blastocysts. Based on these findings, EV exchange may be suggested as an emerging way of communication at the maternal-fetal interface.Elisa GiacominiRiccardo VagoAna Maria SanchezPaola PodiniNatasa ZarovniValentina MurdicaRoberta RizzoDaria BortolottiMassimo CandianiPaola ViganòNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elisa Giacomini Riccardo Vago Ana Maria Sanchez Paola Podini Natasa Zarovni Valentina Murdica Roberta Rizzo Daria Bortolotti Massimo Candiani Paola Viganò Secretome of in vitro cultured human embryos contains extracellular vesicles that are uptaken by the maternal side
description	Abstract Communication between embryo and maternal endometrium occurs during a specific time frame in which implantation is possible. Here we demonstrate for the first time that conditioned media from non-manipulated human embryos cultured in vitro for 3 days or up to the blastocyst stage contain extracellular vesicles (EVs) with a diameter of 50 to 200 nm and bearing the traditional microvesicle and exosome marker proteins CD63, CD9 and ALIX. The embryonic origin of these EVs has been confirmed by the presence of stemness gene transcripts and their enrichment in the non-classical HLA-G protein. NANOG and POU5F1 transcripts were shown to be contained in vesicles deriving from embryos at different stages of development. In line with a higher detection rate of the HLA-G protein in blastocysts compared to cleavage stage embryos, a significantly higher amount of HLA-G was found in vesicles accumulated in spent media from day 3 to day 5 of development compared to those isolated from the earlier stage. Uptake of dye-labeled embryo-derived EVs by human primary endometrial epithelial and stromal cells was also demonstrated with a fluorescence intensity signal significantly higher for cells treated with vesicles derived from blastocysts. Based on these findings, EV exchange may be suggested as an emerging way of communication at the maternal-fetal interface.
format	article
author	Elisa Giacomini Riccardo Vago Ana Maria Sanchez Paola Podini Natasa Zarovni Valentina Murdica Roberta Rizzo Daria Bortolotti Massimo Candiani Paola Viganò
author_facet	Elisa Giacomini Riccardo Vago Ana Maria Sanchez Paola Podini Natasa Zarovni Valentina Murdica Roberta Rizzo Daria Bortolotti Massimo Candiani Paola Viganò
author_sort	Elisa Giacomini
title	Secretome of in vitro cultured human embryos contains extracellular vesicles that are uptaken by the maternal side
title_short	Secretome of in vitro cultured human embryos contains extracellular vesicles that are uptaken by the maternal side
title_full	Secretome of in vitro cultured human embryos contains extracellular vesicles that are uptaken by the maternal side
title_fullStr	Secretome of in vitro cultured human embryos contains extracellular vesicles that are uptaken by the maternal side
title_full_unstemmed	Secretome of in vitro cultured human embryos contains extracellular vesicles that are uptaken by the maternal side
title_sort	secretome of in vitro cultured human embryos contains extracellular vesicles that are uptaken by the maternal side
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/28e2c579bfa94dbd808d6db598e2e8c6
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Secretome of in vitro cultured human embryos contains extracellular vesicles that are uptaken by the maternal side

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