Phenotypic and genomic hallmarks of a novel, potentially pathogenic rapidly growing Mycobacterium species related to the Mycobacterium fortuitum complex

Abstract Previously, we have identified a putative novel rapidly growing Mycobacterium species, referred to as TNTM28, recovered from the sputum of an apparently immunocompetent young man with an underlying pulmonary disease. Here we provide a thorough characterization of TNTM28 genome sequence, whi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Reem Gharbi, Varun Khanna, Wafa Frigui, Besma Mhenni, Roland Brosch, Helmi Mardassi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/28e8d022476c4773be32d36a61fb98ef
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:28e8d022476c4773be32d36a61fb98ef
record_format	dspace
spelling	oai:doaj.org-article:28e8d022476c4773be32d36a61fb98ef2021-12-02T18:02:55ZPhenotypic and genomic hallmarks of a novel, potentially pathogenic rapidly growing Mycobacterium species related to the Mycobacterium fortuitum complex10.1038/s41598-021-91737-82045-2322https://doaj.org/article/28e8d022476c4773be32d36a61fb98ef2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91737-8https://doaj.org/toc/2045-2322Abstract Previously, we have identified a putative novel rapidly growing Mycobacterium species, referred to as TNTM28, recovered from the sputum of an apparently immunocompetent young man with an underlying pulmonary disease. Here we provide a thorough characterization of TNTM28 genome sequence, which consists of one chromosome of 5,526,191 bp with a 67.3% G + C content, and a total of 5193 predicted coding sequences. Phylogenomic analyses revealed a deep-rooting relationship to the Mycobacterium fortuitum complex, thus suggesting a new taxonomic entity. TNTM28 was predicted to be a human pathogen with a probability of 0.804, reflecting the identification of several virulence factors, including export systems (Sec, Tat, and ESX), a nearly complete set of Mce proteins, toxin-antitoxins systems, and an extended range of other genes involved in intramacrophage replication and persistence (hspX, ahpC, sodA, sodC, katG, mgtC, ClpR, virS, etc.), some of which had likely been acquired through horizontal gene transfer. Such an arsenal of potential virulence factors, along with an almost intact ESX-1 locus, might have significantly contributed to TNTM28 pathogenicity, as witnessed by its ability to replicate efficiently in macrophages. Overall, the identification of this new species as a potential human pathogen will help to broaden our understanding of mycobacterial pathogenesis.Reem GharbiVarun KhannaWafa FriguiBesma MhenniRoland BroschHelmi MardassiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Reem Gharbi Varun Khanna Wafa Frigui Besma Mhenni Roland Brosch Helmi Mardassi Phenotypic and genomic hallmarks of a novel, potentially pathogenic rapidly growing Mycobacterium species related to the Mycobacterium fortuitum complex
description	Abstract Previously, we have identified a putative novel rapidly growing Mycobacterium species, referred to as TNTM28, recovered from the sputum of an apparently immunocompetent young man with an underlying pulmonary disease. Here we provide a thorough characterization of TNTM28 genome sequence, which consists of one chromosome of 5,526,191 bp with a 67.3% G + C content, and a total of 5193 predicted coding sequences. Phylogenomic analyses revealed a deep-rooting relationship to the Mycobacterium fortuitum complex, thus suggesting a new taxonomic entity. TNTM28 was predicted to be a human pathogen with a probability of 0.804, reflecting the identification of several virulence factors, including export systems (Sec, Tat, and ESX), a nearly complete set of Mce proteins, toxin-antitoxins systems, and an extended range of other genes involved in intramacrophage replication and persistence (hspX, ahpC, sodA, sodC, katG, mgtC, ClpR, virS, etc.), some of which had likely been acquired through horizontal gene transfer. Such an arsenal of potential virulence factors, along with an almost intact ESX-1 locus, might have significantly contributed to TNTM28 pathogenicity, as witnessed by its ability to replicate efficiently in macrophages. Overall, the identification of this new species as a potential human pathogen will help to broaden our understanding of mycobacterial pathogenesis.
format	article
author	Reem Gharbi Varun Khanna Wafa Frigui Besma Mhenni Roland Brosch Helmi Mardassi
author_facet	Reem Gharbi Varun Khanna Wafa Frigui Besma Mhenni Roland Brosch Helmi Mardassi
author_sort	Reem Gharbi
title	Phenotypic and genomic hallmarks of a novel, potentially pathogenic rapidly growing Mycobacterium species related to the Mycobacterium fortuitum complex
title_short	Phenotypic and genomic hallmarks of a novel, potentially pathogenic rapidly growing Mycobacterium species related to the Mycobacterium fortuitum complex
title_full	Phenotypic and genomic hallmarks of a novel, potentially pathogenic rapidly growing Mycobacterium species related to the Mycobacterium fortuitum complex
title_fullStr	Phenotypic and genomic hallmarks of a novel, potentially pathogenic rapidly growing Mycobacterium species related to the Mycobacterium fortuitum complex
title_full_unstemmed	Phenotypic and genomic hallmarks of a novel, potentially pathogenic rapidly growing Mycobacterium species related to the Mycobacterium fortuitum complex
title_sort	phenotypic and genomic hallmarks of a novel, potentially pathogenic rapidly growing mycobacterium species related to the mycobacterium fortuitum complex
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/28e8d022476c4773be32d36a61fb98ef
work_keys_str_mv	AT reemgharbi phenotypicandgenomichallmarksofanovelpotentiallypathogenicrapidlygrowingmycobacteriumspeciesrelatedtothemycobacteriumfortuitumcomplex AT varunkhanna phenotypicandgenomichallmarksofanovelpotentiallypathogenicrapidlygrowingmycobacteriumspeciesrelatedtothemycobacteriumfortuitumcomplex AT wafafrigui phenotypicandgenomichallmarksofanovelpotentiallypathogenicrapidlygrowingmycobacteriumspeciesrelatedtothemycobacteriumfortuitumcomplex AT besmamhenni phenotypicandgenomichallmarksofanovelpotentiallypathogenicrapidlygrowingmycobacteriumspeciesrelatedtothemycobacteriumfortuitumcomplex AT rolandbrosch phenotypicandgenomichallmarksofanovelpotentiallypathogenicrapidlygrowingmycobacteriumspeciesrelatedtothemycobacteriumfortuitumcomplex AT helmimardassi phenotypicandgenomichallmarksofanovelpotentiallypathogenicrapidlygrowingmycobacteriumspeciesrelatedtothemycobacteriumfortuitumcomplex
_version_	1718378797521174528

Phenotypic and genomic hallmarks of a novel, potentially pathogenic rapidly growing Mycobacterium species related to the Mycobacterium fortuitum complex

Ejemplares similares