Apoptotic Genes’ Expression in an Ovarian Cancer Cell Line (A2780) Exposed to Green Synthesized Cerium Oxide Nanoemulsion

Introduction: Cancer can be defined as an illness of reformed gene expression. There are numerous agents affecting gene expression and altering cellular activities. Nanotechnology has offered the possibility of modulating tumor suppressor genes’ expression, improving kinetics of gene-targeted therap...

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Autores principales:	Mohammad Ashna, Ali Es-haghi, Mojgan Soltani
Formato:	article
Lenguaje:	EN
Publicado:	Zabol University of Medical sciences 2021
Materias:	cerium oxide nanoemulsion apoptotic gene ovarian cancer cell line a2780 Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2901aae803b24398b57356c81a1992c5
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spelling	oai:doaj.org-article:2901aae803b24398b57356c81a1992c52021-11-17T09:55:12ZApoptotic Genes’ Expression in an Ovarian Cancer Cell Line (A2780) Exposed to Green Synthesized Cerium Oxide Nanoemulsion2476-664X10.34172/ijbsm.2021.09https://doaj.org/article/2901aae803b24398b57356c81a1992c52021-06-01T00:00:00Zhttp://ijbsm.zbmu.ac.ir/PDF/ijbsm-21463https://doaj.org/toc/2476-664XIntroduction: Cancer can be defined as an illness of reformed gene expression. There are numerous agents affecting gene expression and altering cellular activities. Nanotechnology has offered the possibility of modulating tumor suppressor genes’ expression, improving kinetics of gene-targeted therapeutics, and simplifying drug delivery to tumors and across bio-complexes.Methods: Gene expressions of caspase3, 8 and 9, Bax and Bcl2 were assessed after RNA extraction and cDNA synthesis by real-time polymerase chain reaction (PCR) in an ovarian cancerous cell line (A2780). Real-time PCR was employed to determine the change fold of apoptotic genes in the cells exposed to the biosynthesized CeO2-NPs (cerium oxide nanoparticles) at the dose of 0, 7, 9, and 11 μg/mL after 24 hours of incubation. Results: Our findings displayed a significant increase in the antioxidant genes’ expression in the A2870 cells. The results exhibited that the biosynthesized CeO2-NPs could enhance the Bax/Bcl2 ratio in a dose-dependent way. Also, the expression of caspase3, 8, and 9 up-regulated significantly under the dose of 11 μg/mL.Conclusion: Considering the effects of the bio-fabricated CeO2-NPs on the expression of apoptotic genes in ovarian cancerous cell lines, these nanoparticles (NPs) may be employed in pharmacology to develop new anti-cancer medications.Mohammad AshnaAli Es-haghiMojgan SoltaniZabol University of Medical sciencesarticlecerium oxide nanoemulsion apoptotic gene ovarian cancer cell line a2780 Medicine (General)R5-920ENInternational Journal of Basic Science in Medicine, Vol 6, Iss 2, Pp 51-56 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cerium oxide nanoemulsion apoptotic gene ovarian cancer cell line a2780 Medicine (General) R5-920
spellingShingle	cerium oxide nanoemulsion apoptotic gene ovarian cancer cell line a2780 Medicine (General) R5-920 Mohammad Ashna Ali Es-haghi Mojgan Soltani Apoptotic Genes’ Expression in an Ovarian Cancer Cell Line (A2780) Exposed to Green Synthesized Cerium Oxide Nanoemulsion
description	Introduction: Cancer can be defined as an illness of reformed gene expression. There are numerous agents affecting gene expression and altering cellular activities. Nanotechnology has offered the possibility of modulating tumor suppressor genes’ expression, improving kinetics of gene-targeted therapeutics, and simplifying drug delivery to tumors and across bio-complexes.Methods: Gene expressions of caspase3, 8 and 9, Bax and Bcl2 were assessed after RNA extraction and cDNA synthesis by real-time polymerase chain reaction (PCR) in an ovarian cancerous cell line (A2780). Real-time PCR was employed to determine the change fold of apoptotic genes in the cells exposed to the biosynthesized CeO2-NPs (cerium oxide nanoparticles) at the dose of 0, 7, 9, and 11 μg/mL after 24 hours of incubation. Results: Our findings displayed a significant increase in the antioxidant genes’ expression in the A2870 cells. The results exhibited that the biosynthesized CeO2-NPs could enhance the Bax/Bcl2 ratio in a dose-dependent way. Also, the expression of caspase3, 8, and 9 up-regulated significantly under the dose of 11 μg/mL.Conclusion: Considering the effects of the bio-fabricated CeO2-NPs on the expression of apoptotic genes in ovarian cancerous cell lines, these nanoparticles (NPs) may be employed in pharmacology to develop new anti-cancer medications.
format	article
author	Mohammad Ashna Ali Es-haghi Mojgan Soltani
author_facet	Mohammad Ashna Ali Es-haghi Mojgan Soltani
author_sort	Mohammad Ashna
title	Apoptotic Genes’ Expression in an Ovarian Cancer Cell Line (A2780) Exposed to Green Synthesized Cerium Oxide Nanoemulsion
title_short	Apoptotic Genes’ Expression in an Ovarian Cancer Cell Line (A2780) Exposed to Green Synthesized Cerium Oxide Nanoemulsion
title_full	Apoptotic Genes’ Expression in an Ovarian Cancer Cell Line (A2780) Exposed to Green Synthesized Cerium Oxide Nanoemulsion
title_fullStr	Apoptotic Genes’ Expression in an Ovarian Cancer Cell Line (A2780) Exposed to Green Synthesized Cerium Oxide Nanoemulsion
title_full_unstemmed	Apoptotic Genes’ Expression in an Ovarian Cancer Cell Line (A2780) Exposed to Green Synthesized Cerium Oxide Nanoemulsion
title_sort	apoptotic genes’ expression in an ovarian cancer cell line (a2780) exposed to green synthesized cerium oxide nanoemulsion
publisher	Zabol University of Medical sciences
publishDate	2021
url	https://doaj.org/article/2901aae803b24398b57356c81a1992c5
work_keys_str_mv	AT mohammadashna apoptoticgenesexpressioninanovariancancercelllinea2780exposedtogreensynthesizedceriumoxidenanoemulsion AT alieshaghi apoptoticgenesexpressioninanovariancancercelllinea2780exposedtogreensynthesizedceriumoxidenanoemulsion AT mojgansoltani apoptoticgenesexpressioninanovariancancercelllinea2780exposedtogreensynthesizedceriumoxidenanoemulsion
_version_	1718425598484807680

Apoptotic Genes’ Expression in an Ovarian Cancer Cell Line (A2780) Exposed to Green Synthesized Cerium Oxide Nanoemulsion

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