THE EFFECTS OF COPPER AND ZINC IONS DURING THEIR BINDING WITH HUMAN SERUM γ-GLOBULIN

Abstract. Conformational changes of human serum γ-globulin were studied during and after its binding with copper and zinc ions, using molecular ultrafiltration and differential spectrophotometry. The contents of nonbound metals in the filtrate were evaluated, resp., with sodium diethyl thyocarbamate...

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Autores principales:	S. B. Cheknev, E. E. Babaeva, A. E. Golub, E. A. Denisova, U. A. Vorobieva
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	γ-globulin copper zinc binding Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/2902918f6eb54a779d6f76f8d20b4887
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spelling	oai:doaj.org-article:2902918f6eb54a779d6f76f8d20b48872021-11-18T08:03:42ZTHE EFFECTS OF COPPER AND ZINC IONS DURING THEIR BINDING WITH HUMAN SERUM γ-GLOBULIN1563-06252313-741X10.15789/1563-0625-2006-5-6-615-622https://doaj.org/article/2902918f6eb54a779d6f76f8d20b48872014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/533https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. Conformational changes of human serum γ-globulin were studied during and after its binding with copper and zinc ions, using molecular ultrafiltration and differential spectrophotometry. The contents of nonbound metals in the filtrate were evaluated, resp., with sodium diethyl thyocarbamate and o-phenanthroline. It has been shown that copper and zinc exhibited common biological properties during their interactions with protein, but the binding differed sufficiently under similar experimental conditions. E.g., it was confirmed that copper was more active at the external sites of γ-globulin molecule, whereas zinc demonstrated tropicity for the areas of protein intraglobular compartments. The metal-binding sites have been described that differ in their parameters of interactions with cations and their spatial location within globular domains. Approaches are suggested for dynamic analysis of saturation for these differently located sites by the metal ions. We discuss the issues of altered conformational state of the γ-globulin molecule during the binding of cations, as well as potential usage of these data in clinical immunology.S. B. CheknevE. E. BabaevaA. E. GolubE. A. DenisovaU. A. VorobievaSPb RAACIarticleγ-globulincopperzincbindingImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 8, Iss 5-6, Pp 615-622 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	γ-globulin copper zinc binding Immunologic diseases. Allergy RC581-607
spellingShingle	γ-globulin copper zinc binding Immunologic diseases. Allergy RC581-607 S. B. Cheknev E. E. Babaeva A. E. Golub E. A. Denisova U. A. Vorobieva THE EFFECTS OF COPPER AND ZINC IONS DURING THEIR BINDING WITH HUMAN SERUM γ-GLOBULIN
description	Abstract. Conformational changes of human serum γ-globulin were studied during and after its binding with copper and zinc ions, using molecular ultrafiltration and differential spectrophotometry. The contents of nonbound metals in the filtrate were evaluated, resp., with sodium diethyl thyocarbamate and o-phenanthroline. It has been shown that copper and zinc exhibited common biological properties during their interactions with protein, but the binding differed sufficiently under similar experimental conditions. E.g., it was confirmed that copper was more active at the external sites of γ-globulin molecule, whereas zinc demonstrated tropicity for the areas of protein intraglobular compartments. The metal-binding sites have been described that differ in their parameters of interactions with cations and their spatial location within globular domains. Approaches are suggested for dynamic analysis of saturation for these differently located sites by the metal ions. We discuss the issues of altered conformational state of the γ-globulin molecule during the binding of cations, as well as potential usage of these data in clinical immunology.
format	article
author	S. B. Cheknev E. E. Babaeva A. E. Golub E. A. Denisova U. A. Vorobieva
author_facet	S. B. Cheknev E. E. Babaeva A. E. Golub E. A. Denisova U. A. Vorobieva
author_sort	S. B. Cheknev
title	THE EFFECTS OF COPPER AND ZINC IONS DURING THEIR BINDING WITH HUMAN SERUM γ-GLOBULIN
title_short	THE EFFECTS OF COPPER AND ZINC IONS DURING THEIR BINDING WITH HUMAN SERUM γ-GLOBULIN
title_full	THE EFFECTS OF COPPER AND ZINC IONS DURING THEIR BINDING WITH HUMAN SERUM γ-GLOBULIN
title_fullStr	THE EFFECTS OF COPPER AND ZINC IONS DURING THEIR BINDING WITH HUMAN SERUM γ-GLOBULIN
title_full_unstemmed	THE EFFECTS OF COPPER AND ZINC IONS DURING THEIR BINDING WITH HUMAN SERUM γ-GLOBULIN
title_sort	effects of copper and zinc ions during their binding with human serum γ-globulin
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/2902918f6eb54a779d6f76f8d20b4887
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THE EFFECTS OF COPPER AND ZINC IONS DURING THEIR BINDING WITH HUMAN SERUM γ-GLOBULIN

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